{"id":4118,"date":"2019-07-31T16:50:25","date_gmt":"2019-07-31T08:50:25","guid":{"rendered":"\/\/www.562part.com\/product\/rosgreentm-h2o2-probe-%e8%bf%87%e6%b0%a7%e5%8c%96%e6%b0%a2%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88\/"},"modified":"2021-11-23T10:08:31","modified_gmt":"2021-11-23T02:08:31","slug":"rosgreentm-h2o2-probe-%e8%bf%87%e6%b0%a7%e5%8c%96%e6%b0%a2%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88","status":"publish","type":"product","link":"\/\/www.562part.com\/product\/rosgreentm-h2o2-probe-%e8%bf%87%e6%b0%a7%e5%8c%96%e6%b0%a2%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88\/","title":{"rendered":"ROSGreenTM H2O2 Probe \u8fc7\u6c27\u5316\u6c22\u8367\u5149\u63a2\u9488"},"content":{"rendered":"

ROSGreen<\/strong>TM<\/strong><\/sup> H<\/strong>2<\/strong><\/sub>O<\/strong>2<\/strong><\/sub> Probe \u8fc7\u6c27\u5316\u6c22\u8367\u5149\u63a2\u9488<\/strong><\/span><\/p>\n

\u641c\u7d22\u5173\u952e\u8bcd\uff1a<\/strong><\/span><\/p>\n

Hydrogen Peroxide \u8fc7\u6c27\u5316\u6c22\uff1bH2DCFDA (DCFH-DA, DCFH)\uff1bBES-H2O2-Ac\uff1bAPF \u6c28\u57fa\u82ef\u57fa\u8367\u5149\u7d20; Mitosox Red;<\/span><\/p>\n

\u540c\u4e49\u540d\uff1a<\/strong><\/span><\/p>\n

ROSGreenTM <\/sup>Hydrogen peroxide Probe\uff1bROSGreenTM<\/sup> Hydrogen peroxide sensor\uff1bROSGreenTM<\/sup> H2O2sensor\uff1b<\/span><\/p>\n

\u4ea7\u54c1\u4fe1\u606f<\/strong><\/span><\/p>\n\n\n\n\n
\u4ea7\u54c1\u540d\u79f0<\/span><\/td>\n\u4ea7\u54c1\u7f16\u53f7 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/td>\n\u89c4\u683c\u00a0 \u00a0<\/span><\/td>\n\u4ef7\u683c\uff08\u5143\uff09 \u00a0<\/span><\/td>\n<\/tr>\n
ROSGreenTMH2O2Probe \u8fc7\u6c27\u5316\u6c22\u8367\u5149\u63a2\u9488 \u00a0 \u00a0<\/span><\/td>\nMX5202-1MG<\/span><\/td>\n1mg<\/span><\/td>\n1580<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4ea7\u54c1\u63cf\u8ff0<\/strong><\/span><\/p>\n

\u8fc7\u6c27\u5316\u6c22\uff08Hydrogen peroxide, H2O2\uff09\u662f\u4e00\u79cd\u53cd\u6620\u6c27\u4ee3\u8c22\u526f\u4ea7\u7269\uff0c\u7528\u4e8e\u8bb8\u591a\u6c27\u5316\u5e94\u6fc0\u76f8\u5173\u6001\u7684\u5173\u952e\u8c03\u8282\u5242\uff0c\u53c2\u4e0e\u4eba\u4f53\u5927\u91cf\u7684\u751f\u7406\u548c\u75c5\u7406\u6d3b\u52a8\uff0c\u6bd4\u5982\u54ee\u5598\u3001\u52a8\u8109\u7ca5\u6837\u786c\u5316\u3001\u7cd6\u5c3f\u75c5\u8840\u7ba1\u75c5\u53d8\u3001\u795e\u7ecf\u6027\u8870\u9000\u75be\u75c5\u548c\u5510\u6c0f\u7efc\u5408\u5f81\u7b49\uff0c\u4f46\u76ee\u524d\u5bf9H2O2\u751f\u6210\u3001\u79ef\u7d2f\u3001\u8fd0\u8f93\u548c\u529f\u80fd\u7684\u5206\u5b50\u673a\u5236\u4ecd\u4e86\u89e3\u5f88\u5c11\uff0c\u6700\u5927\u7684\u9650\u5236\u5728\u4e8e\u7f3a\u4e4f\u7075\u654f\u4e14\u7279\u5f02\u6027H2O2\u63a2\u9488\uff0c\u7528\u4e8e\u6d3b\u7ec6\u80de\u7814\u7a76\u3002\u76ee\u524d\u80fd\u5f97\u5230\u7684H2O2\u53cd\u5e94\u6027\u63a2\u9488\u7684\u7f3a\u9677\u5305\u62ec1\uff09\u6765\u81ea\u5176\u4ed6ROS\u6bd4\u5982\u8fc7\u6c27\u4e9a\u785d\u57fa\u9634\u79bb\u5b50\uff08ONOO-\uff09\u3001\u8fc7\u6c27\u5316\u57fa\uff08ROO\u2022\uff09\u3001\u8d85\u6c27\u5316\u7269\uff08\u2022O2\u2013\uff09\u7684\u80cc\u666f\u8367\u5149\u5e72\u6270\uff1b2\uff09\u5bf9\u5916\u6e90\u6d3b\u5316\u9176\u7684\u9700\u6c42\uff1b3\uff09\u6c34\u7f3a\u4e4f\u6c34\u6eb6\u6027\u6216\u4e0d\u517c\u5bb9\u4e8e\u6c34\u6eb6\u4f53\u7cfb\uff1b4\uff09\u4ee5\u53ca\u7d2b\u5916\u533a\u57df\u7684\u6fc0\u53d1\u5149\u8c31\u7279\u5f81\u7b49\u3002<\/span><\/p>\n

\u672cROSGreenTMH2O2Probe\u6b63\u662f\u514b\u670d\u4ee5\u4e0a\u7f3a\u9677\u5f00\u53d1\u7684\u4e00\u6b3e\u8fc7\u6c27\u5316\u6c22\uff08H2O2\uff09\u9009\u62e9\u6027\u63a2\u9488\u3002\u63a2\u9488\u672c\u8eab\u65e0\u8367\u5149\uff0c\u53ef\u89c1\u5149\u533a\u57df\u5185\u65e0\u5438\u6536\u5cf0\u3002\u4e00\u65e6\u63a5\u89e6H2O2\uff0c\u77ac\u95f4\u6fc0\u53d1\u8367\u5149\u589e\u52a0\u5e76\u4f34\u968f\u53ef\u89c1\u6ce2\u957f\u5438\u6536\u5e26\u7684\u751f\u6210\uff08Ex\/Em= 490\/514nm\uff09\u3002\u56e0\u5176\u4e8c\u5143\u7684\u5438\u6536\/\u53d1\u5c04\u8367\u5149\u53cd\u5e94\uff0c\u6b64\u63a2\u9488\u5177\u6709\u8f83\u5927\u7684\u52a8\u529b\u5b66\u8303\u56f4\u3002\u4e0e\u76f8\u4f3c\u7684ROS\u6bd4\u5982O2\u2013\uff0cNO\uff0c\u6216OCl-\u76f8\u6bd4\uff0cROSGreenTMH2O2Probe\u5bf9H2O2\u5448\u73b0\u51fa\uff1e100\u500d\uff08100-fold\uff09\u7684\u9009\u62e9\u6027\u3002<\/span><\/p>\n

\u4fdd\u5b58\u4e0e\u8fd0\u8f93\u65b9\u6cd5<\/strong><\/span><\/p>\n

\u4fdd\u5b58\uff1a-20\u00baC\u907f\u5149\u5e72\u71e5\u4fdd\u5b58\uff0c\u81f3\u5c111\u5e74\u6709\u6548\u3002<\/span><\/p>\n

\u8fd0\u8f93\uff1a\u51b0\u888b\u8fd0\u8f93\u3002<\/span><\/p>\n

\u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

1\uff09\u672c\u54c1\u7684DMSO\u50a8\u5b58\u6db2\u9700\u5206\u88c5\u6210\u5355\u6b21\u7528\u91cf\u540e\uff0c-20\u00baC\u907f\u5149\u4fdd\u5b58\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u6240\u6709\u5de5\u4f5c\u6db2\u73b0\u914d\u73b0\u7528\u3002<\/span><\/p>\n

2\uff09\u6574\u4e2a\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u6ce8\u610f\u907f\u5149\u3002<\/span><\/p>\n

3\uff09\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/span><\/p>\n

\u4f7f\u7528\u65b9\u6cd5<\/strong><\/span><\/p>\n

1\u3001\u50a8\u5b58\u6db2\u5236\u5907<\/strong><\/span><\/p>\n

\u5c06\u4f4e\u6e29\u4fdd\u5b58\u7684\u4ea7\u54c1\u4ece\u51b0\u7bb1\u53d6\u51fa\uff0c\u7f6e\u4e8e\u5ba4\u6e29\u56de\u6e29\u81f3\u5c1120min\u3002\u4f4e\u901f\u79bb\u5fc33-5min\u3002\u4e4b\u540e\u5f80\u74f6\u5185\u52a0\u5165\u9002\u91cf\u65e0\u6c34DMSO\u5145\u5206\u6eb6\u89e3\u914d\u62102~5mM\u50a8\u5b58\u6db2\uff08\u6bd4\u5982\uff0c1mg ROSGreenTMH2O2Probe\uff08Mw: ~ 600\uff09\u52a0\u5165333\u03bcl DMSO\uff0c\u5145\u5206\u6eb6\u89e3\u6df7\u5300\u540e\u5373\u5f97\u52305mM\u50a8\u5b58\u6db2\uff09\u3002\u6309\u7167\u5355\u6b21\u7528\u91cf\u5206\u88c5\u51bb\u5b58\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\uff0c\u81f3\u5c113\u4e2a\u6708\u7a33\u5b9a\u3002<\/span><\/p>\n

2\u3001\u5de5\u4f5c\u6db2\u51c6\u5907<\/strong><\/span><\/p>\n

\u6b63\u5f0f\u5b9e\u9a8c\u524d\uff0c\u6216\u53d6\u7c89\u672b\u6309\u7167\u3010\u50a8\u5b58\u6db2\u5236\u5907\u3011\u7528DMSO\u6eb6\u89e3\uff0c\u6216\u4e8e\u5ba4\u6e29\u878d\u5316\u4e00\u7ba1DMSO\u50a8\u5b58\u6db2\u3002<\/span><\/p>\n

2.1 \u5bf9\u4e8e\u6eb6\u6db2H2O2\u6d4b\u5b9a\uff0c\u6309\u71672~20\u03bcM\u5de5\u4f5c\u6db2\u6d53\u5ea6\u5236\u59072\u00d7\u5de5\u4f5c\u6db2\uff0c\u752820mM Hepes\u7f13\u51b2\u6db2\u6216\u5176\u4ed6\u7f13\u51b2\u6db2\uff08pH 7.0\uff09\u7a00\u91ca\u50a8\u5b58\u6db2\u3002\u5de5\u4f5c\u6db2\u73b0\u914d\u73b0\u7528\u3002\u5efa\u8bae\u4f7f\u75285\u03bcM\u7ec8\u6d53\u5ea6\u7684ROSGreenTMH2O2Probe\uff0c\u6d4b\u5b9a\u6eb6\u6db2\u4f53\u7cfb\u4e2dH2O2\u6d53\u5ea6\u3002<\/span><\/p>\n

2.2 \u5bf9\u4e8e\u7ec6\u80deH2O2\u6d4b\u5b9a\uff0c\u6309\u71672~20\u03bcM\u5de5\u4f5c\u6db2\u6d53\u5ea6\u5236\u59071\u00d7\u5de5\u4f5c\u6db2\uff0c\u7528\u542b20mM Hepes\u7f13\u51b2\u6db2\u7684HBSS\uff0cpH 7.0 \u6216PBS\u7a00\u91ca\u50a8\u5b58\u6db2\u3002\u5de5\u4f5c\u6db2\u73b0\u914d\u73b0\u7528\u3002<\/span><\/p>\n

3\u3001 \u4e0a\u6e05\u6db2H2O2\u6d4b\u5b9a<\/strong><\/span><\/p>\n

\u3010\u4ee5\u4e0b\u4e3a\u6eb6\u6db2H2O2\u6d4b\u5b9a\u7684\u63a8\u8350\u6b65\u9aa4\uff0c\u4ec5\u4f5c\u53c2\u8003\uff0c\u8bf7\u6839\u636e\u5177\u4f53\u9700\u6c42\u505a\u4f18\u5316\u8c03\u6574\u3002\u3011<\/span><\/p>\n

3.1 \u4e8e96\u5b54\u677f\uff0c\u52a050\u03bcl 2\u00d7ROSGreenTMH2O2Probe\u5de5\u4f5c\u6db2\u5230\u6bcf\u4e2a\u5b54\uff08H2O2\u6807\u51c6\u3001\u7a7a\u767d\u5bf9\u7167\u3001\u5f85\u6d4b\u6837\u672c\uff09\u5185\u4f7f\u603b\u7684\u6d4b\u5b9a\u4f53\u79ef\u4e3a100\u03bcl\/\u5b54\u3002\u3010\u6ce8\u610f\uff1a\u5bf9\u4e8e384\u5b54\u677f\uff0c\u52a025\u03bcl\u6837\u54c1\u548c25\u03bcl 2\u00d7ROSGreenTMH2O2Probe\u5de5\u4f5c\u6db2\uff0c\u4f7f\u5f97\u6d4b\u5b9a\u4f53\u79ef\u4e3a50\u03bcl\/\u5b54\u3002\u3011<\/span><\/p>\n

3.2 \u5ba4\u6e29\u907f\u5149\u5b75\u80b2\u53cd\u5e94\u4f53\u7cfb15~60min\u3002<\/span><\/p>\n

3.3 \u8367\u5149\u9176\u6807\u677f\u5185\u76d1\u6d4b\u7528\u8367\u5149\u589e\u5f3a\uff0cEx\/Em=490\/525nm\u3002<\/span><\/p>\n

3.4 \u7a7a\u767d\u5b54\uff08\u53ea\u542b\u6709\u53cd\u5e94\u7f13\u51b2\u6db2\uff09\u7684\u8367\u5149\u7528\u4f5c\u5bf9\u7167\uff0c\u5176\u4ed6\u5b54\u7684\u8367\u5149\u503c\u9700\u51cf\u6389\u7a7a\u767d\u5bf9\u7167\u5b54\u8367\u5149\u3002<\/span><\/p>\n

4\u3001\u6d3b\u7ec6\u80deH2O2\u6d4b\u5b9a<\/strong><\/span><\/p>\n

\u3010ROSGreenTMH2O2Probe\u80fd\u4e3b\u52a8\u6269\u6563\u8fdb\u5165\u6d3b\u7ec6\u80de\uff0c\u4e14\u80fd\u53cd\u6620\u80de\u5185H2O2\u5fae\u6469\u5c14\u6d53\u5ea6\u53d8\u5316\u3002\u4ee5\u4e0b\u662f\u63a8\u8350\u7684\u6d3b\u7ec6\u80deH2O2\u663e\u5fae\u6210\u50cf\u68c0\u6d4b\u6b65\u9aa4\uff0c\u4ec5\u4f5c\u53c2\u8003\uff0c\u53ef\u6839\u636e\u5177\u4f53\u9700\u6c42\u4f18\u5316\u8c03\u6574\u3002\u3011<\/span><\/p>\n

4.1 \u6309\u7167\u6b65\u9aa42.2\u914d\u52361\u00d7ROSGreenTMH2O2Probe\u5de5\u4f5c\u6db2\u3002\u5de5\u4f5c\u6db2\u73b0\u914d\u73b0\u7528\u3002<\/span><\/p>\n

4.2 \u6309\u7167\u5b9e\u9a8c\u8981\u6c42\u5904\u7406\u7ec6\u80de\u3002<\/span><\/p>\n

4.3 \u75281\u00d7ROSGreenTMH2O2Probe\u5de5\u4f5c\u6db2\u907f\u5149\u5b75\u80b2\u7ec6\u80de5~60min\u6216\u9002\u5408\u81ea\u8eab\u9700\u6c42\u7684\u65f6\u95f4\u3002\u7528PBS\u6e05\u6d17\u7ec6\u80de2\u6b21\u3002<\/span><\/p>\n

4.3 \u7528\u8367\u5149\u9176\u6807\u677f\uff08\u5e95\u90e8\u8bfb\u6570\u6a21\u5f0f\uff09\u76d1\u6d4b\u8367\u5149\u589e\u5f3a\uff0cEx\/Em=490\/525nm\u3002\u6216\u8005\u7528\u8367\u5149\u663e\u5fae\u955c\u7684FITC\u901a\u9053\u6210\u50cf\u68c0\u6d4b\u8367\u5149\u589e\u5f3a\u3002<\/span><\/p>\n

\u67d3\u8272\u793a\u4f8b<\/strong><\/span><\/p>\n

\"\"<\/span><\/p>\n

\u56fe1. Costar \u9ed1\u827296\u5b54\u57f9\u517b\u677f\u5185CHO-K1\u6d3b\u7ec6\u80de\u7528ROSGreenTMH2O2Probe\u7684\u67d3\u8272\u56fe\u3002\u56feA\uff1a\u5bf9\u7167\u7ec6\u80de\uff1b\u56feB\uff1a\u7528H2O2\uff08100\u03bcM\uff09\u5904\u74065min\u540e\u7684\u7ec6\u80de\uff1b<\/span><\/p>\n

\u76f8\u5173\u4ea7\u54c1<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
\u8d27\u53f7<\/span><\/td>\n\u540d\u79f0<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n<\/tr>\n
MX4801-1KIT<\/a> \u00a0 \u00a0 \u00a0 \u00a0<\/span><\/td>\nReactive Oxygen Species (ROS) Assay Kit \u6d3b\u6027\u6c27\uff08ROS\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2<\/a> \u00a0 \u00a0<\/span><\/td>\n100~500T \u00a0\u00a0<\/span><\/td>\n<\/tr>\n
MX4802-50MG<\/a><\/span><\/td>\nH2DCFDA (DCFH-DA, DCFH) \u6d3b\u6027\u6c27\uff08ROS\uff09\u8367\u5149\u63a2\u9488<\/a><\/span><\/td>\n50mg<\/span><\/td>\n<\/tr>\n
MX4802-250MG<\/span><\/td>\nH2DCFDA (DCFH-DA, DCFH) \u6d3b\u6027\u6c27\uff08ROS\uff09\u8367\u5149\u63a2\u9488<\/span><\/td>\n250mg<\/span><\/td>\n<\/tr>\n
MX4804-1MG<\/a><\/span><\/td>\nAminophenyl fluorescein (APF) \u6c28\u57fa\u82ef\u57fa\u8367\u5149\u7d20<\/a><\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
MX4805-1MG<\/a><\/span><\/td>\nHydroxyphenyl fluorescein (HPF) \u7f9f\u82ef\u57fa\u8367\u5149\u7d20<\/a><\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
MM0707-500ML<\/a><\/span><\/td>\n30% H2O2Solution 30%\u8fc7\u6c27\u5316\u6c22\u6eb6\u6db2\uff0830%\u53cc\u6c27\u6c34\uff09<\/a><\/span><\/td>\n500ml<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u66f4\u591a\u4ea7\u54c1\u4fe1\u606f\u8bf7\u54a8\u8be2\u5ba2\u670d\uff01<\/p>\n","protected":false},"featured_media":28906,"template":"","meta":[],"product_cat":[99],"product_tag":[105],"_links":{"self":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product\/4118"}],"collection":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media\/28906"}],"wp:attachment":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media?parent=4118"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product_cat?post=4118"},{"taxonomy":"product_tag","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product_tag?post=4118"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}