{"id":4090,"date":"2019-07-31T16:50:12","date_gmt":"2019-07-31T08:50:12","guid":{"rendered":"\/\/www.562part.com\/product\/reactive-oxygen-species-ros-assay-kit-%e6%b4%bb%e6%80%a7%e6%b0%a7%ef%bc%88ros%ef%bc%89%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e7%9b%92\/"},"modified":"2022-04-11T10:11:46","modified_gmt":"2022-04-11T02:11:46","slug":"reactive-oxygen-species-ros-assay-kit-%e6%b4%bb%e6%80%a7%e6%b0%a7%ef%bc%88ros%ef%bc%89%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e7%9b%92","status":"publish","type":"product","link":"\/\/www.562part.com\/product\/reactive-oxygen-species-ros-assay-kit-%e6%b4%bb%e6%80%a7%e6%b0%a7%ef%bc%88ros%ef%bc%89%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e7%9b%92\/","title":{"rendered":"Reactive Oxygen Species (ROS) Assay Kit \u6d3b\u6027\u6c27\uff08ROS\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2"},"content":{"rendered":"

Reactive Oxygen Species (ROS) Assay Kit <\/strong>\u6d3b\u6027\u6c27\uff08ROS\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2<\/strong><\/span><\/p>\n

\u641c\u7d22\u5173\u952e\u8bcd\uff1a<\/strong><\/span><\/p>\n

Reactive Oxygen Species\uff0cROS\u6d3b\u6027\u6c27\uff1bDCFH-DA\uff1bJC-1\u7ebf\u7c92\u4f53\u6a21\u7535\u4f4d\u63a2\u9488<\/a>\uff1bJC-10\uff1bCAS NO\uff1a4091-99-0\uff1b<\/span><\/p>\n

\u4ea7\u54c1\u8ba2\u8d2d\uff1a<\/strong><\/span><\/p>\n\n\n\n\n
\u00a0 \u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/strong><\/span><\/td>\n\u00a0 \u4ea7\u54c1\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/strong><\/td>\n\u00a0 \u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/strong><\/td>\n\u00a0 \u4ef7\u683c\uff08\u5143\uff09<\/strong> \u00a0 <\/span><\/td>\n<\/tr>\n
\u00a0 Reactive Oxygen Species (ROS) Assay Kit \u6d3b\u6027\u6c27\uff08ROS\uff09\u68c0\u6d4b\u8bd5\u5242\u76d2<\/span><\/span><\/td>\n\u00a0 MX4801-1KIT<\/span><\/td>\n\u00a0 100~500T<\/span><\/td>\n\u00a0 468<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7814\u7a76\u610f\u4e49\uff1a<\/strong><\/span><\/p>\n

\u6d3b\u6027\u6c27\uff08Reactive Oxygen Species\uff0cROS\uff09\u5305\u62ec\u8d85\u6c27\u81ea\u7531\u57fa\u3001\u8fc7\u6c27\u5316\u6c22\u3001\u53ca\u5176\u4e0b\u6e38\u4ea7\u7269\u8fc7\u6c27\u5316\u7269\u548c\u7f9f\u5316\u7269\u7b49\uff0c\u53c2\u4e0e\u7ec6\u80de\u751f\u957f\u589e\u6b96\u3001\u53d1\u80b2\u5206\u5316\u3001\u8870\u8001\u548c\u51cb\u4ea1\u4ee5\u53ca\u8bb8\u591a\u751f\u7406\u548c\u75c5\u7406\u8fc7\u7a0b\u3002<\/span><\/p>\n

\u4ea7\u54c1\u63cf\u8ff0\uff1a<\/strong><\/span><\/p>\n

\u6d3b\u6027\u6c27\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08Reactive Oxygen Species Assay Kit\uff0c\u6216ROS Assay Kit\uff09\u662f\u4e00\u79cd\u5229\u7528\u8367\u5149\u67d3\u6599DCFH<\/a>-DA\uff082,7-Dichlorodihydrofluorescein diacetate\uff09\u8fdb\u884c\u7ec6\u80de\u5185\u6d3b\u6027\u6c27\u6c34\u5e73\u68c0\u6d4b\u7684\u5e38\u7528\u65b9\u6cd5\u3002\u68c0\u6d4b\u539f\u7406\u5728\u4e8eDCFH-DA\u672c\u8eab\u65e0\u8367\u5149\uff0c\u80fd\u81ea\u7531\u7a7f\u8fc7\u7ec6\u80de\u819c\uff0c\u4e00\u65e6\u8fdb\u5165\u7ec6\u80de\u540e\uff0c\u88ab\u80de\u5185\u7684\u916f\u9176\u6c34\u89e3\u751f\u6210\u65e0\u8367\u5149\u7684DCFH\u3002DCFH\u4e0d\u80fd\u7a7f\u900f\u7ec6\u80de\u819c\uff0c\u4ece\u800c\u4fdd\u7559\u5728\u80de\u5185\u3002\u6b64\u65f6\u80de\u5185\u7684\u6d3b\u6027\u6c27\u53ef\u4ee5\u6c27\u5316DCFH\u751f\u6210\u6709\u8367\u5149\u7684DCF\u3002\u901a\u8fc7DCF\u8367\u5149\u7684\u5f3a\u5f31\u5373\u53ef\u53cd\u6620\u6d3b\u6027\u6c27\u7684\u6c34\u5e73\u3002<\/span><\/p>\n

\u672c\u8bd5\u5242\u76d2\u5305\u542b\u6d3b\u6027\u6c27\u9633\u6027\u5bf9\u7167Rosup\uff0c\u5229\u4e8e\u6d3b\u6027\u6c27\u7684\u68c0\u6d4b\u3002Rosup\u662f\u4e00\u79cd\u6df7\u5408\u7269\uff0c\u6d53\u5ea6\u4e3a50mg\/ml\u3002\u56e0\u68c0\u6d4b\u5bf9\u8c61\u548c\u53cd\u5e94\u4f53\u7cfb\u7684\u4e0d\u540c\uff0c\u672c\u8bd5\u5242\u76d2\u53ef\u68c0\u6d4b100-500\u4e2a\u6837\u54c1\u3002<\/span><\/p>\n

\u4ea7\u54c1\u7ec4\u6210\uff1a<\/strong><\/span><\/p>\n\n\n\n\n\n\n
\u00a0\u7ec4\u5206\u7f16\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u00a0\u7ec4\u5206\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/td>\n\u00a0\u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n\u00a0\u50a8\u5b58\u65b9\u6cd5<\/span><\/td>\n<\/tr>\n
\u00a0MX4801-A<\/span><\/td>\n\u00a0\u6d3b\u6027\u6c27\u63a2\u9488DCFH-DA\uff0810mM\uff09<\/span><\/td>\n\u00a00.1ml<\/span><\/td>\n\u00a0-20\u2103\u907f\u5149\u4fdd\u5b58<\/span><\/td>\n<\/tr>\n
\u00a0MX4801-B<\/span><\/td>\n\u00a0\u6d3b\u6027\u6c27\u9633\u6027\u5bf9\u7167\uff08Rosup, 50mg\/ml\uff09<\/span><\/td>\n\u00a01ml<\/span><\/td>\n\u00a0-20\u2103\u4fdd\u5b58<\/span><\/td>\n<\/tr>\n
\u2014 \u2014<\/span><\/td>\n\u00a0\u4ea7\u54c1\u8bf4\u660e\u4e66<\/span><\/td>\n\n

1\u4efd<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4fdd\u5b58\u4e0e\u8fd0\u8f93\u65b9\u6cd5\uff1a<\/strong><\/span><\/p>\n

\u4fdd\u5b58\uff1a-20\u00baC\u4fdd\u5b58\uff0c\u6709\u6548\u671f\u4e00\u5e74\u3002<\/span><\/p>\n

\u8fd0\u8f93\uff1a\u51b0\u888b\u8fd0\u8f93\u3002<\/span><\/p>\n

\u4f7f\u7528\u65b9\u6cd5<\/strong><\/span><\/p>\n

1. \u88c5\u8f7d\u63a2\u9488<\/strong><\/span><\/p>\n

\u3010\u6ce8\u610f\u3011\uff1a<\/strong>\u5bf9\u4e8e\u523a\u6fc0\u65f6\u95f4\u8f83\u77ed\uff08\u901a\u5e382h\u4ee5\u5185\uff09\u7684\u7ec6\u80de\uff0c\u5148\u88c5\u8f7d\u63a2\u9488\uff0c\u540e\u7528\u6d3b\u6027\u6c27\u9633\u6027\u5bf9\u7167Rosup\u548c\/\u6216\u5f85\u7814\u7a76\u836f\u7269\u523a\u6fc0\u7ec6\u80de\uff1b\u5bf9\u4e8e\u523a\u6fc0\u65f6\u95f4\u8f83\u957f\uff08\u901a\u5e386h\u4ee5\u4e0a\uff09\u7684\u7ec6\u80de\uff0c\u5148\u7528\u6d3b\u6027\u6c27\u9633\u6027\u5bf9\u7167Rosup\u6216\u5f85\u7814\u7a76\u836f\u7269\u523a\u6fc0\u7ec6\u80de\uff0c\u540e\u88c5\u8f7d\u63a2\u9488\u3002<\/span><\/p>\n

1.1 \u539f\u4f4d\u88c5\u8f7d\u63a2\u9488\uff08\u4ec5\u9002\u7528\u4e8e\u8d34\u58c1\u7ec6\u80de\uff09<\/strong><\/span><\/p>\n

1\uff09\u68c0\u6d4b\u524d\u4e00\u5929\u8fdb\u884c\u7ec6\u80de\u94fa\u677f\uff0c\u786e\u4fdd\u6d3b\u6027\u6c27\u68c0\u6d4b\u5f53\u5929\u7ec6\u80de\u6c47\u5408\u7387\u8fbe\u523050~70%\u3002<\/span><\/p>\n

2\uff09\u5438\u51fa\u7ec6\u80de\u57f9\u517b\u6db2\uff0c\u52a0\u5165\u9002\u91cf\u4f53\u79ef\u4ee5\u53ca\u6d53\u5ea6\u7684\u5f85\u7814\u7a76\u836f\u7269\uff0c\u4e8e37\u00baC\u7ec6\u80de\u57f9\u517b\u7bb1\u5185\u5b75\u80b2\uff0c\u5177\u4f53\u8bf1\u5bfc\u65f6\u95f4\u6839\u636e\u7ec6\u80de\u7c7b\u578b\u548c\u836f\u7269\u81ea\u8eab\u7279\u6027\u51b3\u5b9a\u3002<\/strong><\/span><\/p>\n

\u3010\u53ef\u9009\u3011<\/strong>\u9633\u6027\u5bf9\u7167Rosup\uff1a<\/strong>\u5148\u7528\u65e0\u8840\u6e05\u57f9\u517b\u6db2\u6309\u71671:1000\u6bd4\u4f8b\u7a00\u91ca\u9633\u6027\u5bf9\u7167\uff0c\u901a\u5e3837\u2103\u57f9\u517b\u7bb1\u5185\u523a\u6fc020~30min\u53ef\u4ee5\u89c2\u5bdf\u5230\u663e\u8457\u7684\u6d3b\u6027\u6c27\u6c34\u5e73\u63d0\u9ad8\uff0c\u4f46\u4f9d\u7ec6\u80de\u7c7b\u578b\u4f1a\u6709\u6bd4\u8f83\u660e\u663e\u5dee\u5f02\u3010\u523a\u6fc0\u65f6\u95f4\u53ef\u53c2\u8003\u6587\u732e\u6216\u5b9e\u9a8c\u7ecf\u9a8c\u6765\u8c03\u6574\u3011\u3002\u5982\u679c\u523a\u6fc0\u540e30min\u5185\u672a\u89c2\u5bdf\u5230\u6d3b\u6027\u6c27\u6c34\u5e73\u5347\u9ad8\uff0c\u53ef\u4ee5\u9002\u5f53\u63d0\u9ad8Rosup\u7684\u5de5\u4f5c\u6d53\u5ea6\uff1b\u53cd\u4e4b\uff0c\u5982\u679c\u6d3b\u6027\u6c27\u5347\u9ad8\u8fc7\u5feb\uff0c\u5219\u9002\u5f53\u964d\u4f4eRosup\u7684\u5de5\u4f5c\u6d53\u5ea6\u3002<\/span><\/p>\n

3\uff09\u6309\u71671:1000\u7528\u65e0\u8840\u6e05\u57f9\u517b\u6db2\u7a00\u91caDCFH-DA\uff0810mM\uff09\uff0c\u4f7f\u5176\u7ec8\u6d53\u5ea6\u4e3a10\u03bcM.\u3002\u5438\u51fa\u7ec6\u80de\u57f9\u517b\u6db2\uff0c\u52a0\u5165\u9002\u5f53\u4f53\u79efDCFH-DA\u5de5\u4f5c\u6db2\u3002\u3010\u6ce8\u610f\u3011\uff1a<\/strong>\u52a0\u5165\u4f53\u79ef\u4ee5\u80fd\u5145\u5206\u76d6\u4f4f\u7ec6\u80de\u4e3a\u5b9c\u3002\u5bf9\u4e8e6\u5b54\u677f\uff0c\u6bcf\u4e2a\u5b54\u52a0\u5165DCFH-DA\u5de5\u4f5c\u6db2\u4e0d\u5c11\u4e8e1ml\u3002\u5bf9\u4e8e96\u5b54\u677f\uff0c\u6bcf\u4e2a\u5b54\u52a0\u5165DCFH-DA\u5de5\u4f5c\u6db2\u4e0d\u5c11\u4e8e0.1ml\u3002<\/span><\/p>\n

4\uff0937\u00baC\u7ec6\u80de\u57f9\u517b\u7bb1\u5185\u5b75\u80b220min\u3002<\/span><\/p>\n

5\uff09\u7528\u65e0\u8840\u6e05\u7ec6\u80de\u57f9\u517b\u6db2\u6d17\u6da4\u7ec6\u80de\u4e09\u6b21\uff0c\u4ee5\u5145\u5206\u53bb\u9664\u672a\u8fdb\u5165\u7ec6\u80de\u5185\u7684DCFH-DA\u3002<\/span><\/p>\n

1.2 \u6536\u96c6\u7ec6\u80de\u540e\u88c5\u8f7d\u63a2\u9488<\/strong><\/span><\/p>\n

1\uff09\u6309\u7167\u5e38\u89c4\u65b9\u6cd5\uff0c\u6e05\u6d17\u5e76\u6536\u96c6\u8db3\u91cf\u7684\u7ec6\u80de\u3002\u3010\u6ce8\u610f\u3011\uff1a<\/strong>\u4fdd\u8bc1\u7ec6\u80de\u7684\u72b6\u6001\u5065\u5eb7\u3002<\/span><\/p>\n

2\uff09\u7528\u9002\u91cf\u4f53\u79ef\u4ee5\u53ca\u6d53\u5ea6\u7684\u5f85\u7814\u7a76\u836f\u7269\u91cd\u65b0\u60ac\u6d6e\u7ec6\u80de\uff0c\u4e8e37\u00baC\u7ec6\u80de\u57f9\u517b\u7bb1\u5185\u5b75\u80b2\uff0c\u5177\u4f53\u8bf1\u5bfc\u65f6\u95f4\u6839\u636e\u7ec6\u80de\u7c7b\u578b\u548c\u836f\u7269\u81ea\u8eab\u7279\u6027\u51b3\u5b9a\u3002\u3010\u53ef\u9009\u3011\u9633\u6027\u5bf9\u7167Rosup\uff1a<\/strong>\u5148\u7528\u65e0\u8840\u6e05\u57f9\u517b\u6db2\u6309\u71671:1000\u6bd4\u4f8b\u7a00\u91ca\u9633\u6027\u5bf9\u7167\uff0c\u901a\u5e3837\u2103\u57f9\u517b\u7bb1\u5185\u523a\u6fc020~30min\u53ef\u4ee5\u89c2\u5bdf\u5230\u663e\u8457\u7684\u6d3b\u6027\u6c27\u6c34\u5e73\u63d0\u9ad8\uff0c\u4f46\u4f9d\u7ec6\u80de\u7c7b\u578b\u4f1a\u6709\u6bd4\u8f83\u660e\u663e\u5dee\u5f02\u3010\u523a\u6fc0\u65f6\u95f4\u53ef\u53c2\u8003\u6587\u732e\u6216\u5b9e\u9a8c\u7ecf\u9a8c\u6765\u8c03\u6574\u3011\u3002\u5982\u679c\u523a\u6fc0\u540e30min\u5185\u672a\u89c2\u5bdf\u5230\u6d3b\u6027\u6c27\u6c34\u5e73\u5347\u9ad8\uff0c\u53ef\u4ee5\u9002\u5f53\u63d0\u9ad8Rosup\u7684\u5de5\u4f5c\u6d53\u5ea6\uff1b\u53cd\u4e4b\uff0c\u5982\u679c\u6d3b\u6027\u6c27\u5347\u9ad8\u8fc7\u5feb\uff0c\u5219\u9002\u5f53\u964d\u4f4eRosup\u7684\u5de5\u4f5c\u6d53\u5ea6\u3002<\/span><\/p>\n

3\uff09\u63a2\u9488\u88c5\u8f7d\u524d\uff0c\u6309\u71671:1000\u7528\u65e0\u8840\u6e05\u57f9\u517b\u6db2\u7a00\u91caDCFH-DA\uff0810mM\uff09\uff0c\u4f7f\u5176\u7ec8\u6d53\u5ea6\u4e3a10\u03bcM.\u3002<\/span><\/p>\n

4\uff09\u79bb\u5fc3\uff0c\u5438\u51fa\u7ec6\u80de\u5185\u523a\u6fc0\u836f\u7269\uff0c\u4e4b\u540e\u7528\u9002\u91cfDCFH-DA\u5de5\u4f5c\u6db2\u91cd\u60ac\u7ec6\u80de\uff0c\u4f7f\u5f97\u7ec6\u80de\u5bc6\u5ea6\u4e3a1\u00d7106~2\u00d7107\/ml\u3002\u3010\u6ce8\u610f\u3011\uff1a<\/strong>\u7ec6\u80de\u5bc6\u5ea6\u9700\u6839\u636e\u540e\u7eed\u68c0\u6d4b\u4f53\u7cfb\uff0c\u68c0\u6d4b\u65b9\u6cd5\uff0c\u4ee5\u53ca\u68c0\u6d4b\u603b\u91cf\u6765\u8c03\u6574\u3002\u4f8b\u5982\uff0c\u5bf9\u4e8e\u6d41\u5f0f\u5206\u6790\uff0c\u5355\u7ba1\u68c0\u6d4b\u5185\u7ec6\u80de\u6570\u76ee\u63a7\u5236\u5728104~ 106\u4e4b\u95f4\u3002<\/span><\/p>\n

5\uff0937\u00baC\u7ec6\u80de\u57f9\u517b\u7bb1\u5185\u5b75\u80b220min\u3002\u6bcf\u96943~5min\u98a0\u5012\u6df7\u5300\u4e00\u4e0b\uff0c\u4f7f\u63a2\u9488\u548c\u7ec6\u80de\u5145\u5206\u63a5\u89e6\u3002<\/span><\/p>\n

6\uff09\u7528\u65e0\u8840\u6e05\u7ec6\u80de\u57f9\u517b\u6db2\u6d17\u6da4\u7ec6\u80de\u4e09\u6b21\uff0c\u4ee5\u5145\u5206\u53bb\u9664\u672a\u8fdb\u5165\u7ec6\u80de\u5185\u7684DCFH-DA\u3002<\/span><\/p>\n

2. \u68c0\u6d4b<\/strong><\/span><\/p>\n

2.1 \u539f\u4f4d\u88c5\u8f7d\u6cd5<\/strong><\/span><\/p>\n

\u53ef\u7528\u6fc0\u5149\u5171\u805a\u7126\u663e\u5fae\u955c\u76f4\u63a5\u89c2\u5bdf\uff0c\u6216\u6536\u96c6\u7ec6\u80de\u540e\u7528\u8367\u5149\u5206\u5149\u5149\u5ea6\u8ba1\u3001\u8367\u5149\u9176\u6807\u4eea\u6216\u6d41\u5f0f\u7ec6\u80de\u4eea\u68c0\u6d4b\u3002<\/span><\/p>\n

2.2 \u6536\u96c6\u7ec6\u80de\u540e\u88c5\u8f7d\u6cd5<\/strong><\/span><\/p>\n

\u53ef\u7528\u8367\u5149\u5206\u5149\u5149\u5ea6\u8ba1\u3001\u8367\u5149\u9176\u6807\u4eea\u6216\u6d41\u5f0f\u7ec6\u80de\u4eea\u68c0\u6d4b\uff0c\u4e5f\u53ef\u7528\u6fc0\u5149\u5171\u805a\u7126\u663e\u5fae\u955c\u76f4\u63a5\u89c2\u5bdf\u3002<\/span><\/p>\n

3. \u53c2\u6570\u8bbe\u7f6e<\/strong><\/span><\/p>\n

\u4f7f\u7528488nm\u6fc0\u53d1\u6ce2\u957f\uff0c525nm\u53d1\u5c04\u6ce2\u957f\uff0c\u5b9e\u65f6\u6216\u9010\u65f6\u95f4\u70b9\u68c0\u6d4b\u523a\u6fc0\u524d\u540e\u8367\u5149\u7684\u5f3a\u5f31\u3002DCF\u7684\u8367\u5149\u5149\u8c31\u548cFITC\u975e\u5e38\u76f8\u4f3c\uff0c\u53ef\u7528FITC\u7684\u53c2\u6570\u8bbe\u7f6e\u68c0\u6d4bDCF\u3002<\/span><\/p>\n

4. \u5176\u4ed6\u8bf4\u660e<\/strong><\/span><\/p>\n

1\uff09\u5bf9\u4e8e\u67d0\u4e9b\u7ec6\u80de\uff0c\u82e5\u53d1\u73b0\u6ca1\u6709\u523a\u6fc0\u7684\u9634\u6027\u5bf9\u7167\u7ec6\u80de\u8367\u5149\u4e5f\u6bd4\u8f83\u5f3a\uff0c\u53ef\u4ee5\u6309\u71671:2000\uff5e1:5000\u7a00\u91caDCFH-DA\uff0c\u4f7fDCFH-DA\u7684\u5de5\u4f5c\u6d53\u5ea6\u4e3a2\uff5e5\u03bcM\u3002\u63a2\u9488\u88c5\u8f7d\u65f6\u95f4\u4e5f\u53ef\u4f9d\u60c5\u51b5\u572815\uff5e60min\u5185\u9002\u5f53\u8fdb\u884c\u8c03\u6574\u3002<\/span><\/p>\n

2\uff09Rosup\u4ec5\u4ec5\u7528\u4e8e\u4f5c\u4e3a\u9633\u6027\u5bf9\u7167\u7684\u6837\u54c1\uff0c\u5e76\u4e0d\u662f\u5728\u6bcf\u4e2a\u6837\u54c1\u4e2d\u90fd\u9700\u52a0\u5165Rosup\u9633\u6027\u5bf9\u7167\u3002<\/span><\/p>\n

\u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

1\uff09\u63a2\u9488\u88c5\u8f7d\u540e\uff0c\u4e00\u5b9a\u8981\u6d17\u51c0\u6b8b\u4f59\u7684\u672a\u8fdb\u5165\u7ec6\u80de\u5185\u7684\u63a2\u9488\uff0c\u5426\u5219\u4f1a\u5bfc\u81f4\u80cc\u666f\u8f83\u9ad8\u3002<\/span><\/p>\n

2\uff09\u63a2\u9488\u88c5\u8f7d\u5b8c\u6bd5\u5e76\u6d17\u51c0\u6b8b\u4f59\u63a2\u9488\u540e\uff0c\u53ef\u4ee5\u8fdb\u884c\u6fc0\u53d1\u6ce2\u957f\u7684\u626b\u63cf\u548c\u53d1\u5c04\u6ce2\u957f\u7684\u626b\u63cf\uff0c\u4ee5\u786e\u8ba4\u63a2\u9488\u7684\u88c5\u8f7d\u60c5\u51b5\u662f\u5426\u826f\u597d\u3002<\/span><\/p>\n

3\uff09\u5c3d\u91cf\u7f29\u77ed\u63a2\u9488\u88c5\u8f7d\u540e\u5230\u6d4b\u5b9a\u6240\u7528\u7684\u65f6\u95f4\uff08\u523a\u6fc0\u65f6\u95f4\u9664\u5916\uff09\uff0c\u4ee5\u51cf\u5c11\u5404\u79cd\u53ef\u80fd\u7684\u8bef\u5dee\u3002<\/span><\/p>\n

4\uff09\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/span><\/p>\n

\u00a0\u2014 \u2014 Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

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