{"id":29249,"date":"2021-08-05T14:44:23","date_gmt":"2021-08-05T06:44:23","guid":{"rendered":"\/\/www.562part.com\/?post_type=product&p=29249"},"modified":"2022-08-22T10:44:18","modified_gmt":"2022-08-22T02:44:18","slug":"directpcr-lysis-reagent-cell-%e7%bb%86%e8%83%9e%e7%9b%b4%e6%8e%a5pcr%e8%a3%82%e8%a7%a3%e6%b6%b2","status":"publish","type":"product","link":"\/\/www.562part.com\/product\/directpcr-lysis-reagent-cell-%e7%bb%86%e8%83%9e%e7%9b%b4%e6%8e%a5pcr%e8%a3%82%e8%a7%a3%e6%b6%b2\/","title":{"rendered":"DirectPCR Lysis Reagent (Cell) \u7ec6\u80de\u76f4\u63a5PCR\u88c2\u89e3\u6db2"},"content":{"rendered":"

DirectPCR Lysis Reagent (Cell) \u7ec6\u80de\u76f4\u63a5PCR\u88c2\u89e3\u6db2<\/strong><\/span><\/p>\n

\u3010\u5355\u74f6\u8bd5\u5242\uff0c\u7ec6\u80de\u88c2\u89e3+PCR\u4e00\u6b65\u5230\u4f4d\u3011<\/strong><\/span><\/p>\n

\"\"<\/span><\/p>\n

\u4ea7\u54c1\u5173\u952e\u8bcd\uff1a<\/strong><\/span><\/p>\n

Viagen Biotech; DirectPCR\u00ae<\/sup>\u00a0\u76f4\u63a5PCR\uff1bPCR genotyping \u57fa\u56e0\u5206\u578b\uff1bProteinase K \u86cb\u767d\u9176K\uff1b302-C (301-C)\uff1b<\/span><\/p>\n

 <\/p>\n

\u4ea7\u54c1\u4ecb\u7ecd\uff1a<\/strong><\/span><\/p>\n

Viagen DirectPCR\u00ae<\/sup>\u00a0DNA Extraction System\uff08DirectPCR\u00ae\u00a0<\/sup>DNA\u63d0\u53d6\u7cfb\u7edf\uff09\u662f\u4e00\u79cd\u5355\u7ba1\u7cfb\u7edf\uff0c\u7528\u4e8e\u4ece\u5c0f\u9f20\u5c3e\u5df4\uff0c\u8033\u7247\uff0c\u5375\u9ec4\u56ca\u548c\u57f9\u517b\u7ec6\u80de\u5185\u5feb\u9012\u5236\u5907DNA\u3002Viagen Biotech, Inc\u79d1\u5b66\u5bb6\u5f00\u53d1\u7684\u4e13\u5229\u7ec4\u5206\uff08\u6b63\u5728\u7533\u8bf7\u4e13\u5229\uff09\u5141\u8bb8\u7528DirectPCR\u00ae DNA Extraction System\u83b7\u5f97\u7684DNA\u63d0\u53d6\u7269\u4e0e\u57fa\u56e0\u5206\u578b\u7528\u7684\u57fa\u56e0\u7ec4PCR\u517c\u5bb9\u3002\u751f\u7269\u6837\u672c\u7684\u7c97\u63d0\u7269\u4e0e\u8bb8\u591a\u5206\u5b50\u751f\u7269\u5b66\u7ea7\u7684\u8bd5\u5242\u6bd4\u5982PCR\u90fd\u96be\u4ee5\u517c\u5bb9\uff0c\u90e8\u5206\u539f\u56e0\u5728\u4e8e\u7c97\u63d0\u7269\u5185\u542b\u6709\u6291\u5236\u5242\u3002DirectPCR\u00ae\u4e0d\u4ec5\u4ecb\u5bfc\u751f\u7269\u6837\u54c1\u7684\u5feb\u901f\u88c2\u89e3\uff0c\u800c\u4e14\u542b\u6709\u6291\u5236\u5242\uff0c\u80fd\u591f\u6709\u6548\u6291\u5236PCR\u6269\u589e\u7528\u7c97\u63d0\u88c2\u89e3\u7269\u4e2d\u5b58\u5728\u7684\u6291\u5236\u6548\u5e94\uff0c\u540c\u65f6\u6700\u5927\u7a0b\u5ea6\u4fdd\u6301\u91ca\u653e\u51fa\u6765\u7684\u57fa\u56e0\u7ec4DNA\u7684\u5b8c\u6574\u6027\u3002\u4e13\u5229\u7533\u8bf7\u4e2d\u7684DirectPCR\u00ae\u64cd\u4f5c\u8d77\u6765\uff0c\u5b8c\u5168\u6d88\u9664\u4efb\u4f55\u6eb6\u6db2\u8f6c\u79fb\u6216\u5f00\u7ba1\u6b65\u9aa4\uff0c\u6839\u672c\u4e0a\u8282\u7701\u4e86\u5927\u91cf\u65f6\u95f4\u548c\u7cbe\u529b\u3002<\/span><\/p>\n

 <\/p>\n

\u4ea7\u54c1\u7279\u70b9\uff1a<\/strong><\/span><\/p>\n

1\uff09\u7701\u65f6\uff1a\u66f4\u5c11\u7684\u52a8\u624b\u65f6\u95f4\uff1b\u5c3e\u5df4\u88c2\u89e3\u6db2\u7c97\u63d0\u7269\u5373\u53ef\u7528\u4e8ePCR\uff1b<\/span><\/p>\n

2\uff09\u7701\u94b1\uff1a\u4ec5\u9700\u6210\u672c\u7684\u53cd\u5e94\u6d41\u7a0b\uff1b<\/span><\/p>\n

3\uff09\u5b89\u5168\uff1a\u65e0\u6709\u673a\u8bd5\u5242\uff1b<\/span><\/p>\n

4\uff09\u73af\u4fdd\uff1a\u66f4\u5c11\u6d88\u8017\uff08\u6709\u673a\u8bd5\u5242\uff0c\u7ba1\u5b50\uff0c\u67aa\u5934\u7b49\u7b49\uff09<\/span><\/p>\n

5\uff09\u53ef\u9760\u6709\u6548\uff1a100%\u6210\u529f\u7387\u4fdd\u8bc1\u9ad8\u4ea7\u91cf<\/span><\/p>\n

 <\/p>\n

\u7b80\u5355\u6d41\u7a0b\uff1a<\/strong><\/span><\/p>\n

1\uff09\u7528DirectPCR\u00ae\u88c2\u89e3\u7ec6\u80de\uff1b<\/span><\/p>\n

2\uff0985\u2103\u5b75\u80b245min\uff1b<\/span><\/p>\n

3\uff09\u53d61\u03bcl\u88c2\u89e3\u6db2\u8fdb\u884cPCR\u57fa\u56e0\u578b\u7814\u7a76\u3002<\/span><\/p>\n

 <\/p>\n

\u8be6\u7ec6\u6d41\u7a0b\uff1a<\/strong><\/span><\/p>\n

1\uff0910cm\u7ec6\u80de\u57f9\u517b\u677f\u7ec6\u80de\uff0c\u4f7f\u7528\u6dfb\u52a0\u65b0\u9c9c\u5236\u5907\u76840.2-0.4mg\/ml\u86cb\u767d\u9176K\u7684200-300\u03bcl DirectPCR\u00aeLysis Reagent (Cell) \u7ec6\u80de\u88c2\u89e3\u6db2\u91cd\u60ac\u7ec6\u80de\u3002\u86cb\u767d\u9176K\u5728DirectPCR\u00ae\u4e2d\u7a33\u5b9a\u4fdd\u5b58~24h\u3002\u5982\u679c\u5904\u7406\u5c11\u91cf\u7ec6\u80de\uff0c\u4f1a\u5f88\u96be\u79f0\u91cf\u86cb\u767d\u9176K\u7c89\u672b\uff0c\u53ef\u76f4\u63a5\u4f7f\u7528Viagen\u63d0\u4f9b\u7684\u57fa\u56e0\u7ec4PCR\u7ea7\u7684\u86cb\u767d\u9176K\u6eb6\u6db2\uff08Cat#501-PK\uff09\uff0c\u4f7f\u7528\u6d53\u5ea6\u4e3a0.5-1.0mg\/ml\uff08\u6bcf1ml DirectPCR\u00ae\u4e2d\u52a0\u516525-50\u03bcl\u86cb\u767d\u9176K\u6eb6\u6db2\uff09\u3002\u3010\u6ce8\u610f\u3011\uff1a\u82e5\u4f7f\u7528\u5c0f\u91cf\u7ec6\u80de\uff0c\u53ef\u7528\u53bb\u79bb\u5b50\u6c34\u7a00\u91caDirectPCR\u00ae\u9ad8\u8fbe10\u500d\uff0c\u7136\u540e\u4f7f\u7528\u5408\u9002\u66f4\u591a\u4f53\u79ef\u7684\u88c2\u89e3\u6db2\u7528\u4e8ePCR\u3002\u89c1\u88681\uff0cDirectPCR\u00aeLysis Reagent (Cell)\u8d77\u59cb\u4f7f\u7528\u6761\u4ef6\u3002<\/span><\/p>\n

2\uff09\u7f6e\u4e8e\u65cb\u8f6c\u6742\u4ea4\u7089\u4e2d\uff0c55\u2103\u8f6c\u52a8\u7ba1\u5b50\u5904\u74065-6h\u6216\u76f4\u5230\u770b\u4e0d\u89c1\u7ec4\u7ec7\u56e2\u3002\u82e5\u6709\u5fc5\u8981\uff0c\u80fd\u8fc7\u591c\u88c2\u89e3\u6837\u672c\uff0c\u4e0d\u4f1a\u5f71\u54cd\u6548\u7387\u3002\u5b8c\u5168\u88c2\u89e3\u81f3\u5173\u91cd\u8981\u3002\u8981\u60f3\u83b7\u5f97\u66f4\u5177\u6709\u91cd\u590d\u6027\u7684\u7ed3\u679c\uff0c\u4e2d\u9014\u6447\u52a8\u4e00\u4e0b\u74f6\u5b50\u4ee5\u66f4\u6362\u7ba1\u5b50\u7684\u4f4d\u7f6e\u4e00\u6b21\uff0c\u6700\u597d\u5728\u5904\u74062-3h\u4e4b\u540e\u3002<\/span><\/p>\n

3\uff09\u6c34\u6d74\u9505\u518585\u2103\u5b75\u80b2\u7c97\u63d0\u6db245min\u3002\u3010\u53ef\u9009\u3011\u4e8e\u6b65\u9aa44\uff09\u524d\u79bb\u5fc310s\u6c89\u6dc0\u6bdb\u53d1\u3002\u7c97\u63d0\u6db2\u53ef\u7f6e\u4e8e-20\u2103\u4fdd\u5b581\u5e74\uff08\u62164\u2103\u4fdd\u5b581\u5468\uff09\uff0c\u4e0d\u4f1a\u964d\u4f4e\u6548\u7387\u3002<\/span><\/p>\n

4\uff09\u6bcf50\u03bcl PCR\u53cd\u5e94\u4f53\u7cfb\u52a0\u51650.5-1.0\u03bcl\u88c2\u89e3\u6db2\u3002\u63a8\u8350PCR\u7528Qiage Hotstar Taq DNA polymerase\u6216Sigma JumpStart Taq DNA polymerase\u3002<\/span><\/p>\n

\u00a0<\/strong><\/span><\/p>\n

\u88681\uff0cDirectPCR\u00aeLysis Reagent (Cell) \u8d77\u59cb\u4f7f\u7528\u6761\u4ef6\u3002<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n
plate (# cells) \u00a0<\/span><\/td>\nTube size (ml) \u00a0\u00a0<\/span><\/td>\nDirectPCR-Cell (\u03bcl) \u00a0\u00a0<\/span><\/td>\nDilution (fold) \u00a0 \u00a0\u00a0<\/span><\/td>\nLysates (\u03bcl)\/50\u03bcl PCR rxn\u00a0<\/span><\/td>\n<\/tr>\n
96 well*<\/span><\/td>\n0.75<\/span><\/td>\n50-70<\/span><\/td>\n1<\/span><\/td>\n1-1.5<\/span><\/td>\n<\/tr>\n
96 well**<\/span><\/td>\n0.75<\/span><\/td>\n60-80<\/span><\/td>\n2<\/span><\/td>\n2-3<\/span><\/td>\n<\/tr>\n
24 well*<\/span><\/td>\n0.75<\/span><\/td>\n70-100<\/span><\/td>\n1<\/span><\/td>\n1-1.5<\/span><\/td>\n<\/tr>\n
6 well*<\/span><\/td>\n0.75<\/span><\/td>\n100-140<\/span><\/td>\n1<\/span><\/td>\n1-1.5<\/span><\/td>\n<\/tr>\n
10cm*<\/span><\/td>\n1.5ml<\/span><\/td>\n200-300<\/span><\/td>\n1<\/span><\/td>\n1-1.5<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

* Lysis using rotation hybridization oven.\u00a0<\/span><\/p>\n

** Lysis using rocking plate. Wash cells in 96-well plate twice with phosphate buffered saline and add 60 \u00b5l DirectPCR Reagent (Cell), which has been 2-fold diluted.\u00a0 Incubate at 55\u00b0C overnight. Place the 96-well plate in the wet chamber and float the chamber on the 85\u00b0C water bath for 1.5 hrs.\u00a0 Pipette up and down several times. Take 1 \u00b5l of lysates for PCR.<\/span><\/p>\n

 <\/p>\n

\u8ba2\u8d2d\u4fe1\u606f\uff1aViagen Biotech\u539f\u88c5\u6b63\u54c1\uff0c\u73b0\u8d27\u4f9b\u5e94\uff0c\u4ea7\u54c1\u9500\u552e\u5230\u5168\u7403\u4e0a\u5343\u5bb6\u516c\u53f8\u548c\u79d1\u7814\u5355\u4f4d\uff0c\u5305\u62ecMerck\uff0cFisher\uff0cGentaur\uff0cCosmo, Applied stemcell, Genetech\uff0cpFizer\u7b49\u7b49\u3002\u6b22\u8fce\u6765\u7535\u54a8\u8be2\uff0c021-54736159\uff0cQQ:2716000500\u3002<\/strong><\/span><\/p>\n\n\n\n\n\n\n
\u54c1\u724c<\/span><\/td>\n\u540d\u79f0<\/span><\/td>\n\u8d27\u53f7 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/td>\n\u89c4\u683c \u00a0 \u00a0\u00a0\u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/td>\n<\/tr>\n
Viagen Biotech \u00a0 \u00a0 \u00a0\u00a0<\/span><\/td>\nDirectPCR Lysis Reagent (Cell)<\/span><\/td>\n301-C<\/span><\/td>\n50ml<\/span><\/td>\n<\/tr>\n
Viagen Biotech<\/span><\/td>\nDirectPCR Lysis Reagent (Cell))<\/span><\/td>\n302-C<\/span><\/td>\n100ml<\/span><\/td>\n<\/tr>\n
Viagen Biotech<\/span><\/td>\nGenomic PCR Grade Proteinase K Solution \u00a0 \u00a0 \u00a0 \u00a0<\/span><\/td>\n501-PK<\/span><\/td>\n5ml<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e0b\u8f7d\uff1aDirectPCR Lysis Reagent (Cell) Protocol Manual<\/a><\/span><\/p>\n

 <\/p>\n

\u00a0<\/strong><\/span><\/p>\n

\u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

For use with cultured cells\uff01<\/p>\n","protected":false},"featured_media":29250,"template":"","meta":[],"product_cat":[275],"product_tag":[276],"_links":{"self":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product\/29249"}],"collection":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media\/29250"}],"wp:attachment":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media?parent=29249"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product_cat?post=29249"},{"taxonomy":"product_tag","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product_tag?post=29249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}