{"id":26307,"date":"2020-03-27T15:46:39","date_gmt":"2020-03-27T07:46:39","guid":{"rendered":"\/\/www.562part.com\/?post_type=product&p=26307"},"modified":"2023-09-19T13:35:31","modified_gmt":"2023-09-19T05:35:31","slug":"c11-bodipy-581-591","status":"publish","type":"product","link":"\/\/www.562part.com\/product\/c11-bodipy-581-591\/","title":{"rendered":"C11 BODIPY 581\/591 \u8102\u8d28\u8fc7\u6c27\u5316\u63a2\u9488"},"content":{"rendered":"

C11 BODIPY 581\/591 \u8102\u8d28\u8fc7\u6c27\u5316\u8367\u5149\u63a2\u9488<\/strong><\/span><\/p>\n

\u4ea7\u54c1\u6807\u7b7e<\/strong><\/span><\/p>\n

C11 BODIPY 581\/591\uff1blipid peroxidation \u8102\u8d28\u8fc7\u6c27\u5316\uff1bferroptosis \u94c1\u6b7b\u4ea1\uff1bFeRhoNox-1 (Fe2+indicator) \u4e9a\u94c1\u79bb\u5b50\u8367\u5149\u63a2\u9488\uff1bMitoPerOx\u7ebf\u7c92\u4f53\u8102\u8d28\u8fc7\u6c27\u5316\uff1bCAS NO\uff1a217075-36-0\uff1b<\/span><\/p>\n

\u4ea7\u54c1\u4fe1\u606f\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/strong>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/p>\n\n\n\n\n
\n

\u4ea7\u54c1\u540d\u79f0<\/span><\/p>\n<\/td>\n

\u4ea7\u54c1\u7f16\u53f7<\/span><\/td>\n\u00a0 \u89c4\u683c\u00a0 \u00a0<\/span><\/td>\n\u4ef7\u683c\uff08\u5143\uff09<\/span><\/td>\n<\/tr>\n
C11 BODIPY 581\/591 \u8102\u8d28\u8fc7\u6c27\u5316\u8367\u5149\u63a2\u9488\u00a0\u00a0<\/span><\/td>\nMX5211-1MG<\/span><\/td>\n1mg<\/span><\/td>\n\n

2648<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4ea7\u54c1\u63cf\u8ff0<\/strong><\/span><\/p>\n

C11 BODIPY 581\/591\u662f\u4e00\u79cd\u8102\u6eb6\u6027\u7684\u6bd4\u7387\u578b\u8367\u5149\u63a2\u9488\uff0c\u5728\u6a21\u5f0f\u819c\u7cfb\u7edf\u548c\u6d3b\u7ec6\u80de\u5185\u7528\u6765\u6307\u793a\u8102\u8d28\u8fc7\u6c27\u5316\u548c\u6297\u6c27\u5316\u6027\u80fd\u3002<\/span><\/p>\n

C11 BODIPY 581\/591\u5177\u4f18\u79c0\u7684\u7279\u5f81<\/strong>\uff1a\u2460\u53d1\u5c04\u6ce2\u957f\u5728\u7535\u78c1\u6ce2\u8c31\u7684\u53ef\u89c1\u5149\u533a\u57df\uff0c\u975e\u6c27\u5316\u6001\uff08591nm\uff09\u548c\u6c27\u5316\u6001\uff08510nm\uff09\u4e24\u79cd\u5f62\u5f0f\u7684\u5149\u8c31\u80fd\u591f\u5f88\u597d\u7684\u5206\u5f00\uff1b\u2461\u5177\u9ad8\u91cf\u5b50\u4ea7\u91cf\uff0c\u6b63\u56e0\u5982\u6b64\uff0c\u4f7f\u7528\u4f4e\u6807\u8bb0\u6d53\u5ea6\u5373\u53ef\uff0c\u786e\u4fdd\u5bf9\u819c\u4ea7\u751f\u6700\u4f4e\u7684\u5e72\u6270\uff0c\u4e14\u7ef4\u6301\u826f\u597d\u7684\u4fe1\u566a\u6bd4\uff1b\u2462\u5177\u6709\u826f\u597d\u7684\u5149\u7a33\u5b9a\u6027\uff0c\u4ea7\u751f\u5f88\u4f4e\u7684\u8367\u5149\u4f2a\u5f71\uff1b\u2463\u57fa\u672c\u4e0a\u5bf9\u73af\u5883\u53d8\u5316\u6bd4\u5982pH\u6216\u6eb6\u5242\u6781\u6027\u4e0d\u654f\u611f\uff1b\u2464\u4eb2\u8102\u6027\uff0c\u8f7b\u677e\u8fdb\u5165\u819c\uff1b\u2465\u4e00\u65e6\u6c27\u5316\uff0c\u63a2\u9488\u4fdd\u6301\u4eb2\u8102\u6027\uff0c\u4e14\u4e0d\u4f1a\u81ea\u53d1\u5730\u79bb\u5f00\u8102\u8d28\u53cc\u5206\u5b50\u5c42\uff1b\u2466\u975e\u6c27\u5316\u6001\u548c\u6c27\u5316\u6001\u5b9a\u4f4d\u5728\u8102\u8d28\u53cc\u5206\u5b50\u5c42\u5185\u4e24\u4e2a\u4e0d\u540c\u7684\u533a\u57df\uff0c\u4e00\u79cd\u4f4d\u4e8e\u6d45\u5c42\uff08\u79bb\u53cc\u5206\u5b50\u5c42\u4e2d\u5fc3\u8ddd\u79bb\u4e3a18\u00c5\uff09\uff0c\u4e00\u79cd\u4f4d\u4e8e\u6df1\u5c42\uff08\u79bb\u53cc\u5206\u5b50\u5c42\u4e2d\u5fc3\u8ddd\u79bb\u4e3a< 7.5\u00c5\uff09\uff1b\u2467\u9ad8\u8fbe50\u00b5M\u7684\u6d53\u5ea6\u5bf9\u5927\u9f20\u6210\u7ea4\u7ef4\u7ec6\u80de\u65e0\u6bd2\u6027\uff1b\u2468\u5bf9\u5404\u79cd\u6c27\u81ea\u7531\u57fa\u548c\u8fc7\u6c27\u4e9a\u785d\u9178\u76d0\u7075\u654f\uff0c\u4f46\u5bf9\u8d85\u6c27\u5316\u7269\u3001\u4e00\u6c27\u5316\u6c2e\u3001\u8fc7\u6e21\u94c1\u79bb\u5b50\u548c\u6c22\u8fc7\u6c27\u5316\u7269\u4e0d\u7075\u654f\uff1b\u2469\u5176\u5bf9\u6c27\u5316\u7684\u7075\u654f\u5ea6\u4e0e\u5185\u6e90\u6027\u8102\u80aa\u9170\u57fa\u56e2\u76f8\u5f53[1]\u3002C11 BODIPY 581\/591\u53ef\u7528\u6765\u5b9a\u91cf\u94c1\u6b7b\u4ea1[2-3]\u3002<\/span><\/p>\n

\u200b<\/span><\/p>\n

\u4ea7\u54c1\u7279\u6027<\/strong><\/span><\/p>\n

    \n
  1. 1\uff09CAS NO.\uff1a217075-36-0<\/span><\/li>\n
  2. 2\uff09\u5316\u5b66\u540d\uff1a(T-4)-difluoro[5-[[5-[(1E,3E)-4-phenyl-1,3-butadien-1-yl]-2H-pyrrol-2-ylidene-\u03baN]methyl]-1H-pyrrole -2-undecanoato(2-)-\u03baN1]-borate(1-), monohydrogen<\/span><\/li>\n
  3. 3\uff09\u5206\u5b50\u5f0f\uff1aC30H35BF2N2O2<\/span><\/li>\n
  4. 4\uff09\u5206\u5b50\u91cf\uff1a504.4<\/span><\/li>\n
  5. 5\uff09\u7eaf\u5ea6\uff1a\u226595%<\/span><\/li>\n
  6. 6\uff09\u6fc0\u53d1\u6ce2\u957f\uff1a581nm\uff08\u8fd8\u539f\u578b\uff09\uff0c500nm\uff08\u6c27\u5316\u578b\uff09<\/span><\/li>\n
  7. 7\uff09\u53d1\u5c04\u6ce2\u957f\uff1a591nm\uff08\u8fd8\u539f\u578b\uff09\uff0c510nm\uff08\u6c27\u5316\u578b\uff09<\/span><\/li>\n
  8. 8\uff09\u00a0\u00a0\u5316\u5b66\u7ed3\u6784\u5f0f\uff1a\"\"<\/span><\/li>\n<\/ol>\n

    \u4fdd\u5b58\u4e0e\u8fd0\u8f93\u65b9\u6cd5<\/strong><\/span><\/p>\n

    \u4fdd\u5b58\uff1a-20\u00baC\u907f\u5149\u5e72\u71e5\u4fdd\u5b58\uff0c2\u5e74\u6709\u6548\u3002<\/span><\/p>\n

    \u8fd0\u8f93\uff1a\u51b0\u888b\u8fd0\u8f93\u3002<\/span><\/p>\n

    \u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

    1\uff09\u8367\u5149\u67d3\u6599\u5747\u5b58\u5728\u6dec\u706d\u95ee\u9898\uff0c\u8bf7\u5c3d\u91cf\u6ce8\u610f\u907f\u5149\uff0c\u4ee5\u51cf\u7f13\u8367\u5149\u6dec\u706d\u3002<\/span><\/p>\n

    2\uff09\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/span><\/p>\n

    \u5e94\u7528\u793a\u4f8b\uff08\u6765\u81ea\u6587\u732e\uff0c\u4ec5\u505a\u53c2\u8003\uff09<\/strong><\/span><\/p>\n

    1\uff09\u4e3a\u4e86\u6d4b\u5b9a\u8102\u8d28\u6d3b\u6027\u6c27\uff08Lipid ROS\uff09\uff0cMEFs\u7ec6\u80de\u7ecf\u836f\u7269\uff08Erastin 1 \u03bcM\uff1bBafilomycin A1 20nM\uff1bChloroquine 50 \u03bcM\uff09\u9884\u5904\u7406\u540e\uff0c\u4e4b\u540e\u52a0\u5165C11 BODIPY 581\/ 591\uff0850 \u03bcM\uff09<\/strong>\u5b75\u80b21h\u3002\u7528PBS\u6e05\u6d17\u7ec6\u80de\u4e24\u6b21\u4ee5\u53bb\u9664\u591a\u4f59\u7684\u67d3\u6599\u3002\u4e4b\u540e\u7ec6\u80de\u7528\u80f0\u9176\u6d88\u5316\uff0c\u91cd\u60ac\u5728\u542b5% FBS\u7684PBS\u4e2d\u3002C11 BODIPY 581\/ 591\u7684\u591a\u4e0d\u9971\u548c\u4e01\u4e8c\u70ef\u90e8\u5206\u88ab\u6c27\u5316\u5bfc\u81f4\u5176\u6700\u5927\u53d1\u5c04\u8367\u5149\u4ece~590nm\u8fc1\u79fb\u5230~510nm\uff0c\u4e0e\u8102\u8d28\u6d3b\u6027\u6c27\u4ea7\u751f\u6210\u6b63\u6bd4\uff0c\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u4eea\u6765\u5206\u6790\u3002\u3010\u6587\u732e\u6765\u6e90\u3011\uff1aGao M et al. Ferroptosis is an autophagic cell death process. Cell Res. MKBIO, 2016 Sep;26(9):1021-32.<\/span><\/p>\n

    2\uff09\u4e3a\u4e86\u8bc4\u4f30\u8102\u8d28\u8fc7\u6c27\u5316\uff0c\u7ec6\u80de\u7528C11 BODIPY 581\/ 591\uff082 \u03bcM in HEPES-buffered HBSS\uff09<\/strong>\u5b75\u80b220min\uff0c\u4e4b\u540e\u5171\u805a\u7126\u6210\u50cf\uff0c\u7528488nm\u548c565nm\u6fc0\u5149\u5668\u6fc0\u53d1\uff0c\u68c0\u6d4b505-550nm\u548c\uff1e580nm\u4e0b\u7684\u8367\u5149\u3002C11 BODIPY 581\/ 591\u672c\u8d28\u4e0a\u662f\u4e00\u79cd\u4eb2\u8102\u67d3\u6599\uff0c\u80fd\u79ef\u7d2f\u5728\u819c\u5185\u3002\u4e00\u65e6\u67d3\u6599\u7684\u591a\u4e0d\u9971\u548c\u4e01\u4e8c\u70ef\u90e8\u5206\u88ab\u6c27\u5316\uff0c\u6700\u5927\u53d1\u5c04\u6ce2\u957f\u4ece590nm\u8fc1\u79fb\u5230510nm\uff0c\u63a2\u9488\u4ecd\u7ef4\u6301\u4eb2\u8102\u6027\uff0c\u4ece\u800c\u53cd\u6620\u819c\u7684\u8102\u8d28\u8fc7\u6c27\u5316\u6c34\u5e73\u3002\u3010\u6587\u732e\u6765\u6e90\uff1aAngelova, P.R., et al. Alpha synuclein aggregation drives ferroptosis: an interplay of iron, calcium and lipid peroxidation. MKBio; Cell Death Differ (2020).\u3011<\/span><\/p>\n

     <\/p>\n

    \u76f8\u5173\u4ea7\u54c1<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n
    \u8d27\u53f7<\/span><\/td>\n\u540d\u79f0<\/span><\/td>\n\u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
    MX5211-1MG\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\nC11 BODIPY 581\/591 \u8102\u8d28\u8fc7\u6c27\u5316\u8367\u5149\u63a2\u9488<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
    MX4558-50UG<\/span><\/td>\nFeRhoNox-1 (Fe2+indicator) \u4e9a\u94c1\u79bb\u5b50\u8367\u5149\u63a2\u9488<\/span><\/td>\n50\u03bcg<\/span><\/td>\n<\/tr>\n
    MX4559-24UG<\/span><\/td>\nFerroOrange (Fe2+indicator) \u4e9a\u94c1\u79bb\u5b50\u8367\u5149\u63a2\u9488<\/span><\/td>\n24\u03bcg<\/span><\/td>\n<\/tr>\n
    MX5401-1MG<\/span><\/td>\nMitoPerOx Mitochondrial Lipid Peroxidation Indicator \u7ebf\u7c92\u4f53\u8102\u8d28\u8fc7\u6c27\u5316\u63a2\u9488\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n
    MX5402-1MG<\/span><\/td>\nBODIPY 558\/568 C12 \u8102\u8d28\u8f6c\u8fd0\u8367\u5149\u63a2\u9488<\/span><\/td>\n1mg<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

     <\/p>\n

    \u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u9e92\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

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