{"id":16651,"date":"2019-08-12T10:38:33","date_gmt":"2019-08-12T02:38:33","guid":{"rendered":"\/\/www.562part.com\/product\/%e6%9e%9c%e8%83%b6%e9%85%b6y-23-pectolase-y-23-yakult-japan-%e5%8e%9f%e8%a3%85%e6%ad%a3%e5%93%81\/"},"modified":"2023-06-05T14:43:39","modified_gmt":"2023-06-05T06:43:39","slug":"%e6%9e%9c%e8%83%b6%e9%85%b6y-23-pectolase-y-23-yakult-japan-%e5%8e%9f%e8%a3%85%e6%ad%a3%e5%93%81","status":"publish","type":"product","link":"\/\/www.562part.com\/product\/%e6%9e%9c%e8%83%b6%e9%85%b6y-23-pectolase-y-23-yakult-japan-%e5%8e%9f%e8%a3%85%e6%ad%a3%e5%93%81\/","title":{"rendered":"\u679c\u80f6\u9176Y-23 [Pectolase Y-23] Yakult Japan \u539f\u88c5\u6b63\u54c1"},"content":{"rendered":"

\u679c\u80f6\u9176Y-23[Pectolase Y-23] Yakult Japan\u539f\u88c5\u6b63\u54c1<\/strong> <\/span><\/p>\n

\u54c1\u724c\u6545\u4e8b\"\"\uff1a<\/strong><\/span><\/p>\n

\u65e5\u672c\u56fd\u682a\u5f0f\u4f1a\u793eYakult\u672c\u793e\uff08YAKULT HONSHA CO., LTD\uff09\u521b\u4e1a\u4e8e1935\u5e74\uff0c\u8bbe\u7acb\u4e8e1955\u5e744\u67089\u65e5\uff0c\u81f3\u4eca\u62e5\u670970\u591a\u5e74\u751f\u4ea7\u548c\u9500\u552e\u53d1\u9175\u578b\u4e73\u5236\u54c1\u7684\u5386\u53f2\uff0c\u662f\u4e16\u754c\u4e0a\u77e5\u540d\u7684\u751f\u4ea7\u548c\u9500\u552e\u53d1\u9175\u578b\u6d3b\u6027\u4e73\u9178\u83cc\u4e73\u996e\u54c1\u7684\u5236\u9020\u5546\u4e4b\u4e00\u3002Yakult\u96c6\u56e2\u4e0b\u5c5e\u5171\u670940\u591a\u4e2a\u5b50\u516c\u53f8\u3002\u5176\u4e2d\uff0cYakult Pharmaceutical Industry Co., Ltd.\u4e3b\u8981\u751f\u4ea7\u9176\uff0c\u4f4e\u805a\u534a\u4e73\u7cd6\u7b49\uff0c\u5176\u63d0\u4f9b\u7684\u7ea4\u7ef4\u7d20\u9176\uff0c\u79bb\u6790\u9176\u53ca\u679c\u80f6\u9176\u53d7\u5230\u5168\u4e16\u754c\u79d1\u7814\u5de5\u4f5c\u8005\u7684\u4eb2\u7750\u3002\u4e0d\u4ec5\u63d0\u4f9b\u5b9e\u9a8c\u7814\u7a76\u7ea7\u522b\uff0c\u8fd8\u63d0\u4f9b\u5de5\u4e1a\u7ea7\u522b\u7684\u6d88\u5316\u9176\u3002<\/span><\/p>\n

\u6211\u53f8\u96c6\u5947\u751f\u7269\u63d0\u4f9b\u7684\u4e3a\u79d1\u7814\u7ea7\u522b\u7684CELLULASE “ONOZUKA”R-10\uff0cCellulase \u201cOnozuka\u201d RS\uff0cMacerozyme R-10\u548cPectolase Y-23\u3002<\/strong><\/span><\/p>\n

\u679c\u80f6\u9176Y-23 [PECTINASE Y-23]\uff1a<\/strong><\/span><\/p>\n\n\n\n\n\n\n
\u5206\u7c7b\uff1a\u00a0\u00a0\u00a0\u00a0 \u00a0<\/span><\/td>\n\u679c\u80f6\u9176\uff08Pectinase\uff09\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
\u6765\u6e90\uff1a<\/span><\/td>\n\u65e5\u672c\u66f2\u9709\uff08Aspergillus japonicus\uff09<\/span><\/td>\n<\/tr>\n
\u7279\u70b9\uff1a<\/span><\/td>\n\u964d\u89e3\u690d\u7269\u7ec6\u80de\u58c1\uff0c\u9176\u6d3b\u6027\u22651000U\/g solid<\/span><\/td>\n<\/tr>\n
\u5e94\u7528\uff1a<\/span><\/td>\n\u690d\u7269\u539f\u751f\u8d28\u4f53\u5236\u5907<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5e94\u7528\u5b9e\u4f8b\uff1a<\/strong><\/span><\/p>\n\n\n\n\n
\n

\"\"<\/span><\/p>\n

Protoplasts of Carrot<\/strong><\/span><\/p>\n<\/td>\n

\n

\"\"<\/span><\/p>\n

Protoplasts from garden pepper leaf <\/strong><\/span><\/p>\n

and periwinkle <\/strong>suspension culture at <\/strong><\/span><\/p>\n

an early stage of fusion.<\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\"\"<\/span><\/p>\n

Living leaf cells of tobacco isolated using <\/strong><\/span><\/p>\n

MACEROZYME.<\/strong><\/span><\/p>\n<\/td>\n

\n

\"\"<\/span><\/p>\n

Protoplasts of tobacco leaf cells <\/strong><\/span><\/p>\n

prepared using <\/strong>CELLULASE “ONOZUKA”.<\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u8ba2\u8d2d\u4fe1\u606f\uff1aYakult Japan\u539f\u88c5\u8fdb\u53e3\u7684\u5404\u79cd\u690d\u7269\u7ec6\u80de\u58c1\u6d88\u5316\u9176\uff0c\u73b0\u8d27\u4f9b\u5e94\uff0c\u6b22\u8fce\u9009\u8d2d\u3002\u8054\u7cfb\u7535\u8bdd\uff1a021-54736159\uff0cQQ\uff1a2971634497\u3002<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n
\u54c1\u724c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/strong><\/span><\/td>\n\u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\u4ea7\u54c1\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\u4ef7\u683c\uff08\u5143\uff09\u00a0 <\/strong><\/span><\/td>\n\u8d27\u671f\u00a0\u00a0<\/strong>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
Yakult\uff08Japan\uff09<\/span><\/td>\nMX7354<\/span><\/td>\nPectolase Y-23\u679c\u80f6\u9176Y-23<\/span><\/td>\n1g<\/span><\/td>\n1600<\/span><\/td>\n\u73b0\u8d27<\/span><\/td>\n<\/tr>\n
\u5176\u4ed6\u76f8\u5173\u4ea7\u54c1<\/strong><\/span><\/td>\n<\/tr>\n
Yakult\uff08Japan\uff09<\/span><\/td>\nMX7351<\/span><\/td>\nMacerozyme R-10\u79bb\u6790\u9176R-10<\/span><\/td>\n10g<\/span><\/td>\n1600<\/span><\/td>\n\u73b0\u8d27<\/span><\/td>\n<\/tr>\n
Yakult\uff08Japan\uff09<\/span><\/td>\nMX7352<\/span><\/td>\nCellulase R-10\u7ea4\u7ef4\u7d20\u9176R-10<\/span><\/td>\n10g<\/span><\/td>\n1600<\/span><\/td>\n\u73b0\u8d27<\/span><\/td>\n<\/tr>\n
Yakult\uff08Japan\uff09<\/span><\/td>\nMX7353<\/span><\/td>\nCellulase RS\u7ea4\u7ef4\u7d20\u9176RS<\/span><\/td>\n10g<\/span><\/td>\n3450<\/span><\/td>\n\u73b0\u8d27<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u3010\u5907\u6ce8\u3011\uff1a\u7ea4\u7ef4\u7d20\u9176RS\u5e38\u5e38\u4e0e\u79bb\u6790\u9176R-10\u8054\u5408\u4f7f\u7528\uff0c\u964d\u89e3\u690d\u7269\u7ec6\u80de\u58c1\u4ee5\u5236\u5907\u539f\u751f\u8d28\u4f53\u3002<\/strong><\/span><\/p>\n

\u9176\u6eb6\u6db2\u5236\u5907\u53c2\u8003\u8d44\u6599\u3010\u4ec5\u505a\u4ea4\u6d41\u5b66\u4e60\u3011\uff1a<\/strong><\/span><\/p>\n

Sheen, J. 2002, A transient expression assay using Arabidopsis mesophyll protoplasts.<\/span><\/p>\n

Digestion buffer<\/strong><\/span><\/p>\n

1%(w\/v) cellulase “onozuka”R10<\/span><\/p>\n

0.25%(w\/v) macerozyme R10<\/span><\/p>\n

0.4 M mannitol<\/span>
\n20 mM MES (pH 5.7)<\/span>
\n20 mM KCl <\/span>
\n10 mM CaCl2<\/span><\/p>\n

Preparation<\/strong><\/span><\/p>\n

1. Add 100 mg of cellulase R10 and 25 mg of macerozyme R10 into a 50 ml tube.<\/span><\/p>\n

2. Add 10 ml of buffer containing 0.4 M mannitol, 20 mM MES (pH 5.7), 20 mM KCl, 10 mM CaCl2into the tube.<\/span><\/p>\n

3. Vortex the tube briefly, and shake in orbit at room temperature for few min (2-5 min).<\/span>
\n\u00a0\u00a0\u00a0 *The digestion solution will be clear (light brown).<\/span>
\n\u00a0\u00a0\u00a0 **No heat treatment and No filtration<\/span><\/p>\n

\u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/span><\/p>\n

 <\/p>\n

\u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u672c\u53f8YAKULT\u4ea7\u54c1\u5747\u4e3a\u539f\u88c5\u6b63\u54c1\uff0c\u4ea7\u54c1\u8d27\u671f\u8bf7\u54a8\u8be2\u5ba2\u670d\u786e\u8ba4\uff01<\/p>\n","protected":false},"featured_media":26464,"template":"","meta":[],"product_cat":[95],"product_tag":[185],"_links":{"self":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product\/16651"}],"collection":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media\/26464"}],"wp:attachment":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media?parent=16651"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product_cat?post=16651"},{"taxonomy":"product_tag","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/product_tag?post=16651"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}