{"id":402,"date":"2019-07-24T16:51:56","date_gmt":"2019-07-24T08:51:56","guid":{"rendered":"\/\/www.562part.com\/?p=402"},"modified":"2019-08-09T18:38:01","modified_gmt":"2019-08-09T10:38:01","slug":"%e6%87%8b%e5%ba%b7%e7%94%9f%e7%89%a9%e9%85%b5%e6%af%8d%e5%8f%8c%e6%9d%82%e4%ba%a4%e7%b3%bb%e5%88%97%e4%ba%a7%e5%93%81%e4%bc%98%e6%83%a0%e6%b4%bb%e5%8a%a8%e5%85%a8%e9%9d%a2%e6%8e%a8%e5%bc%80","status":"publish","type":"post","link":"\/\/www.562part.com\/news\/402\/","title":{"rendered":"\u61cb\u5eb7\u751f\u7269\u9175\u6bcd\u53cc\u6742\u4ea4\u7cfb\u5217\u4ea7\u54c1\u4f18\u60e0\u6d3b\u52a8\u5168\u9762\u63a8\u5f00"},"content":{"rendered":"
\u68c0\u6d4b\u539f\u7406\uff1a<\/strong><\/span><\/p>\n \u9175\u6bcd\u53cc\u6742\u4ea4\u7cfb\u7edf\uff08Yeast two-hybrid assay\uff09\u662f\u7528\u4e8e\u4f53\u5185\u7814\u7a76\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7684\u4e00\u79cd\u975e\u5e38\u4fbf\u5229\u7684\u5de5\u5177\uff0c\u5e38\u7528\u7684\u5982GAL4\u4e3a\u57fa\u7840\u7684\u7cfb\u7edf\uff0c\u4f7f\u7528\u9175\u6bcd\u8f6c\u5f55\u56e0\u5b50GAL4\u6765\u68c0\u6d4b\u8f6c\u5f55\u6fc0\u6d3b\u540e\u7684\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u3002\u67d0\u4e9b\u8f6c\u5f55\u56e0\u5b50\uff08\u5982GAL4\uff09\u7531\u4e24\u4e2a\u53ef\u4ee5\u5206\u5f00\uff0c\u529f\u80fd\u4e0a\u76f8\u4e92\u72ec\u7acb\u7684\u7ed3\u6784\u57df\u7ec4\u6210\uff0cN\u7aef\u6709\u4e00\u4e2a147\u4e2a\u6c28\u57fa\u9178\u7ec4\u6210\u7684DNA\u7ed3\u5408\u57df\uff08DNA binding domain\uff0cBD\uff09\uff0cC\u7aef\u6709\u4e00\u4e2a\u7531113\u4e2a\u6c28\u57fa\u9178\u7ec4\u6210\u7684\u8f6c\u5f55\u6fc0\u6d3b\u57df\uff08transcription activation domain\uff0cAD\uff09\u3002BD\u53ef\u4ee5\u548c\u4e0a\u6e38\u6fc0\u6d3b\u5e8f\u5217\uff08UAS\uff09\u7ed3\u5408\uff0c\u800cAD\u80fd\u6fc0\u6d3bUAS\u4e0b\u6e38\u57fa\u56e0\u8fdb\u884c\u8f6c\u5f55\u3002\u5355\u72ec\u7684BD\u6216AD\u4e0d\u80fd\u6fc0\u6d3b\u57fa\u56e0\u8f6c\u5f55\uff0c\u4e24\u8005\u53ea\u6709\u901a\u8fc7\u67d0\u79cd\u65b9\u5f0f\u7ed3\u5408\u5728\u4e00\u8d77\u5f62\u6210\u5b8c\u6574\u7684\u8f6c\u5f55\u6fc0\u6d3b\u56e0\u5b50\u7684\u529f\u80fd\u3010\u89c1\u56fe1\u3011\u3002<\/strong>\u9175\u6bcd\u53cc\u6742\u4ea4\u7cfb\u7edf\u4e3b\u8981\u5229\u7528\u9175\u6bcdGAL4\u7684\u8fd9\u4e2a\u7279\u6027\u901a\u8fc7\u4e24\u4e2a\u6742\u4ea4\u86cb\u767d\u5728\u9175\u6bcd\u7ec6\u80de\u4e2d\u7684\u76f8\u4e92\u7ed3\u5408\u53ca\u5bf9\u62a5\u544a\u57fa\u56e0\u7684\u8f6c\u5f55\u6fc0\u6d3b\u6765\u7814\u7a76\u6d3b\u7ec6\u80de\u5185\u86cb\u767d\u8d28\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u5bf9\u86cb\u767d\u8d28\u4e4b\u95f4\u5fae\u5f31\u3001\u77ac\u95f4\u7684\u4f5c\u7528\u90fd\u80fd\u901a\u8fc7\u62a5\u544a\u57fa\u56e0\u654f\u611f\u7684\u68c0\u6d4b\u5230\u3002<\/p>\n <\/p>\n \u9175\u6bcd\u53cc\u6742\u4ea4\u7cfb\u7edf\u5e94\u7528\uff1a<\/strong><\/span><\/p>\n \u5bf9\u65b0\u7684\u4e0e\u5df2\u7ecf\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7684\u9274\u5b9a<\/p>\n<\/li>\n \u5bf9\u9884\u6d4b\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7684\u786e\u8ba4<\/p>\n<\/li>\n \u5bf9\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u533a\u57df\u7684\u9274\u5b9a<\/p>\n<\/li>\n<\/ol>\n <\/p>\n <\/p>\n \u56fe1. \u53cc\u6742\u4ea4\u68c0\u6d4b\u539f\u7406\u56fe\u3002<\/strong>\u4e24\u4e2a\u86cb\u767d\u5206\u522b\u8868\u8fbe\uff0c\u4e00\u4e2a\uff08\u8bf1\u9975\u86cb\u767dbait protein\uff09\u878d\u5408\u5230Gal4 DNA\u7ed3\u5408\u57df\u8868\u8fbe\uff0c\u53e6\u4e00\u4e2a\uff08\u8bf1\u6355\u86cb\u767dprey protein\uff09\u878d\u5408\u5230Gal4\u8f6c\u5f55\u6fc0\u6d3b\u7ed3\u6784\u57df\uff08AD\uff09\u8868\u8fbe\u3002\u5728Y2HGold\u9175\u6bcd\u83cc\u682a\u4e2d\uff0c\u53ea\u6709\u5f53\u4e24\u4e2a\u86cb\u767d\u4e4b\u95f4\u76f8\u4e92\u4f5c\u7528\u5e76\u7ed3\u5408\u5230Gal4\u53cd\u5e94\u6027\u542f\u52a8\u5b50\u4e0a\u624d\u80fd\u6d3b\u5316\u62a5\u544a\u57fa\u56e0\uff08AUR1-C, ADE2, HIS3,\u548cMEL1\uff09\u7684\u8868\u8fbe\u3002<\/p>\n <\/p>\n \u9175\u6bcd\u53cc\u6742\u4ea4\u7cfb\u7edf\u91cd\u8981\u5143\u4ef6\u4ecb\u7ecd\uff08\u4ee5Matchmarker GAL4-based two hybrid assay\u4e3a\u4f8b\uff09<\/strong><\/span><\/p>\n 1\u3001<\/strong>\u8bf1\u9975\uff08the bait\uff09<\/strong><\/p>\n \u4e3a\u4e86\u5efa\u7acbGAL4 DNA-BD\/bait\u878d\u5408\u86cb\u767d\uff0c\u63a8\u8350\u4f7f\u7528\u8d28\u7c92pGBKT7\uff1b<\/p>\n \u8981\u8c03\u67e5\u4e09\u5143\u86cb\u767d\u590d\u5408\u7269\uff0c\u63a8\u8350\u4f7f\u7528\u542b2\u4e2aMCS\u533a\u57df\u7684\u4e09\u6742\u4ea4\u8f7d\u4f53\uff0c\u80fd\u8868\u8fbeGAL4 DNA-BD\/bait\u878d\u5408\u86cb\u767d\u548c\u7b2c\u4e8c\u4e2a\u611f\u5174\u8da3\u86cb\u767d\uff0c\u5728bait\u548cprey\u86cb\u767d\u4e4b\u95f4\u53d1\u6325\u6865\u6881\u4f5c\u7528\u3002<\/p>\n 2\u3001\u68c0\u6d4b\u86cb\u767d\u4f5c\u7528\u7684\u56db\u4e2a\u62a5\u544a\u57fa\u56e0<\/strong><\/p>\n Matchmarker gold\u7cfb\u7edf\u662f\u91c7\u75284\u4e2a\u62a5\u544a\u57fa\u56e0\/3\u4e2a\u4e0d\u540cbait\u8bc6\u522b\u5e8f\u5217\u7684\u53cc\u6742\u4ea4\u7cfb\u7edf\uff08\u89c1\u56fe2\uff09\u3002\u5176\u91c7\u7528\u65b0\u578b\u7a33\u5b9a\u7684\u9175\u6bcd\u53cc\u6742\u4ea4\u6297\u751f\u7d20-\u91d1\u62c5\u5b50\u7d20Aureobasidin A\uff08AbA\uff09\uff08\u8d27\u53f7\uff1aMS0002-1ML\uff09\uff0c<\/a><\/u><\/strong>\u53ef\u6709\u6548\u6740\u6b7b\u975e\u6297\u6027\u514b\u9686\uff0c\u4ece\u800c\u5927\u5927\u964d\u4f4e\u80cc\u666f\u514b\u9686\u751f\u957f\u3002\u540c\u65f6\u6709\u6548\u7f29\u51cf\u5047\u9633\u6027\uff0c\u7531\u4e8e\u4eba\u548cprey\u86cb\u767d\u5355\u72ec\u5c06\u7ed3\u5408\u5f15\u8d77\u5047\u9633\u6027\u90fd\u9700\u8981\u8bc6\u522b3\u4e2a\u4e0d\u540c\u5e8f\u5217\uff0c\u6fc0\u6d3b4\u4e2a\u62a5\u544a\u57fa\u56e0\u7684\u8868\u8fbe\u3002<\/p>\n 4\u4e2a\u62a5\u544a\u57fa\u56e0\u5206\u522b\u662f\uff1a1\u4e2aAbA\u6297\u751f\u7d20\u7b5b\u9009\u62a5\u544a\u57fa\u56e0\uff0c2\u4e2a\u8425\u517b\u7b5b\u9009\u62a5\u544a\u57fa\u56e0\uff0c1\u4e2a\u84dd\u767d\u6591\u7b5b\u9009\u62a5\u544a\u57fa\u56e0\u3002<\/p>\n 1\uff09<\/strong>AUR1-C<\/strong><\/p>\n AUR1\u57fa\u56e0\u7684\u4e3b\u8981\u7a81\u53d8\u7248\uff0c\u80fd\u591f\u8868\u8fbe\u808c\u9187\u78f7\u8102\u9170\u795e\u7ecf\u9170\u80fa\u5408\u6210\u9176\uff08Inositol phosphorylceramide (IPC) synthase\uff09\u3002\u5f53\u86cb\u767d\u53d1\u751f\u4f5c\u7528\u4fc3\u4f7fGAL4\u8f6c\u5f55\u6fc0\u6d3b\uff0cAUR1-C\u57fa\u56e0\u8fdb\u884c\u8868\u8fbe\u3002\u917f\u9152\u9175\u6bcd\u4e2d\u6b64\u57fa\u56e0\u7684\u8868\u8fbe\u5b75\u80b2\u5176\u5bf9\u9ad8\u6bd2\u6297\u751f\u7d20\u91d1\u62c5\u5b50\u7d20Aureobasidin A\uff08AbA\uff09\u7684\u6297\u6027\u3002\u7531\u4e8e\u5176\u80cc\u666f\u6781\u5176\u4f4e\uff0c\u8fd9\u4e00\u7b5b\u9009\u62a5\u544a\u5b50\u4f18\u4e8e\u5355\u4e00\u7684\u8425\u517b\u7f3a\u9677\u62a5\u544a\u5b50\u3002<\/p>\n 2\uff09HIS3<\/strong><\/p>\n Y2HGold\u83cc\u682a\u4e0d\u80fd\u5408\u6210\u7ec4\u6c28\u9178\uff0c\u4ece\u800c\u65e0\u6cd5\u5728\u7f3a\u5c11\u8fd9\u4e00\u5fc5\u987b\u6c28\u57fa\u9178\u7684\u57f9\u517b\u57fa\u4e0a\u751f\u957f\u3002\u5f53\u8bf1\u9975\u548c\u8bf1\u6355\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\uff0cGal4\u53cd\u5e94\u6027\u7684His3\u8868\u8fbe\u4fc3\u4f7f\u7ec6\u80de\u5408\u6210\u7ec4\u6c28\u9178\u5e76\u80fd\u5728His\u7f3a\u9677\u57f9\u517b\u57fa\u4e0a\u751f\u957f\u3002<\/p>\n 3\uff09ADE2<\/strong><\/p>\n Y2HGold\u83cc\u682a\u65e0\u6cd5\u5728\u4e0d\u542b\u817a\u560c\u5464\u7684\u57fa\u672c\u57f9\u517b\u57fa\u4e0a\u751f\u957f\uff0c\u4f46\u5f53\u4e24\u4e2a\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\uff0cAde2\u8868\u8fbe\u88ab\u6d3b\u5316\uff0c\u7ec6\u80de\u80fd\u5728Ade\u7f3a\u9677\u7684\u57fa\u7840\u57f9\u517b\u57fa\u4e0a\u751f\u957f\u3002<\/p>\n 4\uff09MEL1<\/strong><\/p>\n MEL1\u7f16\u7801\u03b1-\u534a\u4e73\u7cd6\u82f7\u9176\uff0c\u5b58\u5728\u8bb8\u591a\u9175\u6bcd\u83cc\u5185\u7684\u5929\u7136\u8868\u8fbe\u86cb\u767d\u3002\u5f53\u53cc\u6742\u4ea4\u53d1\u751f\uff0cMEL1\u8868\u8fbe\u548c\u5206\u6ccc\uff0c\u5728\u5e95\u7269X-\u03b1-Gal\uff08\u8d27\u53f7\uff1aMF2003-25MG\uff09<\/a><\/u><\/strong>\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\uff0c\u9175\u6bcd\u83cc\u843d\u53d8\u4e3a\u84dd\u8272\u3002<\/p>\n 3\u3001\u4e09\u4e2a\u4e0d\u540c\u7684\u7ed3\u5408\u4f4d\u70b9\uff08three different binding sites\uff09<\/strong><\/p>\n Y2HGold\u83cc\u682a\u4e09\u4e2a\u542f\u52a8\u5b50\u63a7\u52364\u4e2a\u62a5\u544a\u57fa\u56e0\u662f\u4e0d\u76f8\u5173\u7684\uff0c\u9664UAS\u533a\u57df\u7684\u77ed\u86cb\u767d\u7ed3\u5408\u4f4d\u70b9\u7279\u5f02\u6027\u7ed3\u5408Gal4 DNA-BD\u3002\u56e0\u6b64\uff0c\u8bf1\u6355\u86cb\u767d\uff08\u6587\u5e93\u86cb\u767d\u6216\u9776\u86cb\u767d\uff09\u4e0eUAS\u5185\u90e8\u6216\u8fb9\u7f18\u7684\u4e0d\u76f8\u5173\u5e8f\u5217\u4f5c\u7528\uff08\u5047\u9633\u6027\uff09\u5c31\u4f1a\u81ea\u52a8\u88ab\u7b5b\u67e5\u51fa\u6765\u3002<\/p>\n <\/p>\n \u56fe2. Matchmarker\u9175\u6bcd\u83cc\u682a\u7684\u62a5\u544a\u57fa\u56e0\u6784\u9020\u3002<\/strong>Y2HGold\u4e2d\uff0cHIS3\uff0cADE2\uff0cMEL1\uff0c\u548cAUR1-C\u62a5\u544a\u57fa\u56e0\u5206\u522b\u7531\u4e09\u4e2a\u5b8c\u5168\u5f02\u6e90\u7684Gal4\u53cd\u5e94\u542f\u52a8\u5143\u4ef6-G1\uff0cG2\uff0cM1\u64cd\u63a7\u3002\u800c\u542f\u52a8\u5b50\u5185\u7684\u86cb\u767d\u7ed3\u5408\u4f4d\u70b9\u662f\u4e0d\u540c\u7684\uff0c\u867d\u7136\u6bcf\u4e00\u4e2a\u7ed3\u5408\u4f4d\u70b9\u4e0eGal4\u8bc6\u522b\u768417-mer\u5171\u6709\u5e8f\u5217\u76f8\u5173\u8054\u3002<\/p>\n \u9175\u6bcd\u53cc\u6742\u4ea4\u7cfb\u7edf\u64cd\u4f5c\u6d41\u7a0b\uff08\u4ee5Matchmarker GAL4-based two hybrid assay\u4e3a\u4f8b\uff09<\/strong><\/span><\/p>\n \u5b8c\u6574\u7684Matchmarker\u7b5b\u9009\u8fc7\u7a0b\u5305\u542b\u4ee5\u4e0b\u6b65\u9aa4\uff1a<\/p>\n \u7b2c\u4e00\u6b65\uff1a\u6267\u884c\u5bf9\u7167\u5b9e\u9a8c\uff1b<\/p>\n \u7b2c\u4e8c\u6b65\uff1a\u8bf1\u9975\u8f7d\u4f53\u7684\u6784\u5efa\u53ca\u81ea\u6211\u6fc0\u6d3b\u548c\u6bd2\u6027\u68c0\u6d4b\uff1b<\/p>\n \u7b2c\u4e09\u6b65\uff1a\u7b5b\u9009Mate&Plate\u6587\u5e93<\/p>\n \u7b2c\u56db\u6b65\uff1a\u786e\u8ba4\u548c\u9610\u91ca\u7ed3\u679c<\/p>\n <\/p>\n \u56fe3.\u4f7f\u7528Mate&Plate\u6587\u5e93\u8fdb\u884c\u53cc\u6742\u4ea4\u7b5b\u9009\u3002<\/strong>\u6355\u83b7\u86cb\u767d\uff08bait protein\uff09\u662f\u9175\u6bcd\u83ccY2HGold\u8868\u8fbe\u7684Gal4 DNA-BD\u878d\u5408\u86cb\u767d\u3002\u9ad8\u590d\u6742\u6027\u6587\u5e93\u8868\u8fbeGal4 AD\u878d\u5408\u86cb\u767d\uff0c\u7531Y187\u9175\u6bcd\u83cc\u63d0\u4f9b\u3002\u5f53\u4e24\u79cd\u8f6c\u5316\u83cc\u682a\u6df7\u5408\u8fc7\u591c\u80fd\u63a5\u5408\u4ea7\u751f\u4e8c\u500d\u4f53\uff08Diploids\uff09\u3002\u6b64\u4e8c\u500d\u4f53\u542b\u67094\u4e2a\u62a5\u544a\u57fa\u56e0\uff1aHIS3\uff0cADE2\uff0cMEL1\u548cAUR1-C\uff0c\u53d7\u53cc\u6742\u4ea4\u4f5c\u7528\u800c\u6d3b\u5316\u8868\u8fbe\u3002<\/p>\n <\/p>\n \u9175\u6bcd\u53cc\u6742\u4ea4\u68c0\u6d4b\u9700\u8981\u4ea7\u54c1\u4fe1\u606f\uff1a<\/strong><\/p>\n \u6211\u53f8\uff08\u61cb\u5eb7\u751f\u7269\uff09\u63d0\u4f9b\u9175\u6bcd\u53cc\u6742\u4ea4\u7814\u7a76\u9700\u8981\u7684\u4e00\u7cfb\u5217\u4ea7\u54c1\uff0c\u4ece\u9175\u6bcd\u83cc\u682a\u3001\u9175\u6bcd\u8d28\u7c92\u3001\u9175\u6bcd\u57f9\u517b\u57fa\u5230\u7b5b\u9009\u6807\u5fd7\uff08\u6297\u751f\u7d20\uff09\uff0c\u6b22\u8fce\u6765\u7535\u54a8\u8be2\u3002<\/p>\n \u4e00\u3001\u5bcc\u96c6\u57f9\u517b\u57fa\uff08Rich Medium\u9175\u6bcd\u57f9\u517b\uff09<\/strong><\/span><\/p>\n \u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u4ef7\u683c\uff08\u5143\uff09\u00a0 \u00a0\u00a0\u00a0<\/p>\n<\/td>\n<\/tr>\n YPD Agar Medium YPD\u743c\u8102\u57f9\u517b\u57fa <\/a><\/p>\n<\/td>\n 350g<\/p>\n<\/td>\n 550<\/p>\n<\/td>\n<\/tr>\n MS6302-250G<\/p>\n<\/td>\n YPD Medium YPD\u6db2\u4f53\u57f9\u517b\u57fa<\/p>\n<\/td>\n 250g<\/p>\n<\/td>\n 438<\/p>\n<\/td>\n<\/tr>\n YPDA Agar Medium YPDA\u743c\u8102\u57f9\u517b\u57fa <\/a><\/p>\n<\/td>\n 350g<\/p>\n<\/td>\n 550<\/p>\n<\/td>\n<\/tr>\n MS6304-250G<\/p>\n<\/td>\n YPDA Medium YPDA\u6db2\u4f53\u57f9\u517b\u57fa<\/p>\n<\/td>\n 250g<\/p>\n<\/td>\n 438<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n \u3010\u8865\u5145\u63cf\u8ff0\u3011\uff1a<\/strong><\/p>\n 1\uff09YPD\u662f\u7528\u4e8e\u5927\u591a\u6570\u9175\u6bcd\u83cc\u751f\u957f\u6240\u9700\u7684\u9175\u6bcd\u63d0\u53d6\u7269\u3001\u86cb\u767d\u80e8\u548c\u8461\u8404\u7cd6\u4f18\u5316\u914d\u6bd4\u540e\u7ec4\u6210\u7684\u6df7\u5408\u7269\uff1b<\/p>\n 2\uff09YPDA\u57f9\u517b\u57fa\u662f\u5728YPD\u57fa\u7840\u4e0a\u6dfb\u52a0\u786b\u9178\u817a\u560c\u5464\uff0c\u7ec8\u6d53\u5ea6120 \u00b5g\/ml\u3002<\/p>\n \u4e8c\u3001\u57fa\u672c\u57f9\u517b\u57fa\uff08Minimal Medium\u9175\u6bcd\u57f9\u517b\uff09<\/strong><\/span><\/p>\n \u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u89c4\u683c\u00a0\u00a0 \u00a0\u00a0<\/p>\n<\/td>\n \u4ef7\u683c\uff08\u5143\uff09<\/p>\n<\/td>\n<\/tr>\n Minimal SD Agar Base SD\u743c\u8102\u57fa\u7840\u57f9\u517b\u57fa <\/a><\/p>\n<\/td>\n 467g<\/p>\n<\/td>\n 1428<\/p>\n<\/td>\n<\/tr>\n MS6312-267G<\/p>\n<\/td>\n Minimal SD Base SD\u57fa\u7840\u57f9\u517b\u57fa<\/p>\n<\/td>\n 267g<\/p>\n<\/td>\n 768<\/p>\n<\/td>\n<\/tr>\n Minimal SD Agar Base Gal\/Raf SD\u743c\u8102\u57fa\u7840\u57f9\u517b\u57fa\uff08\u542b\u534a\u4e73\u7cd6\/\u68c9\u5b50\u7cd6\uff09<\/p>\n<\/td>\n 270g<\/p>\n<\/td>\n 1428<\/p>\n<\/td>\n<\/tr>\n MS6314-185G<\/p>\n<\/td>\n Minimal SD Base Gal\/Raf SD\u57fa\u7840\u57f9\u517b\u57fa\uff08\u542b\u534a\u4e73\u7cd6\/\u68c9\u5b50\u7cd6\uff09<\/p>\n<\/td>\n 185g<\/p>\n<\/td>\n 988<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n \u3010\u8865\u5145\u63cf\u8ff0\u3011\uff1a<\/strong><\/p>\n 1\uff09SD Medium\uff1a\u9175\u6bcd\u83cc\u7528\u7684\u57fa\u672c\u7684\u5408\u6210\u9650\u5b9a\u57f9\u517b\u57fa\uff08Minimal synthetically defined medium\uff09\uff0c\u5305\u542b\u6c2e\u6e90\uff0c\u78b3\u6e90\uff08\u8461\u8404\u7cd6\uff0c\u9664\u975e\u6709\u7279\u6b8a\u8bf4\u660e\uff09\u548cDO\u6dfb\u52a0\u5242\uff08Dropout Supplement\uff09\u3002<\/p>\n 2\uff09DO\uff08Dropout Supplement or Solution\uff09\uff1a\u7279\u5b9a\u6c28\u57fa\u9178\u548c\u6838\u82f7\u7684\u6df7\u5408\u7269\uff0c\u6dfb\u52a0\u5230SD base\u5185\u4ee5\u914d\u5236SD Medium\u3002DO\u7f3a\u5c11\u4e00\u79cd\u6216\u8005\u66f4\u591a\u672a\u8f6c\u5316\u9175\u6bcd\u83cc\u9700\u8981\u7684\u8425\u517b\u6210\u5206\u4f7f\u5176\u5728SD Medium\u4e0a\u751f\u957f\uff0c\u4f5c\u4e3a\u8425\u517b\u7b5b\u9009\u6807\u5fd7\u3002\u00a0<\/p>\n \u4e09\u3001\u7701\u5374\u6210\u5206\u6c28\u57fa\u9178\u6df7\u5408\u7269\uff08DO Supplements \u9175\u6bcd\u57f9\u517b\uff09<\/strong><\/span><\/p>\n \u7c7b\u578b<\/p>\n<\/td>\n \u540d\u79f0<\/p>\n<\/td>\n \u8d27\u53f7<\/p>\n<\/td>\n \u89c4\u683c \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/p>\n<\/td>\n \u4ef7\u683c\uff08\u5143\uff09\u00a0<\/p>\n<\/td>\n<\/tr>\n Single Dropout Supplements (SDO)<\/p>\n \u5355\u4e00\u7701\u5374\u6210\u5206\u6dfb\u52a0\u5242<\/p>\n<\/td>\n DO Supplement -His<\/p>\n<\/td>\n MS6316-10G \u00a0 \u00a0<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Leu<\/p>\n<\/td>\n MS6317-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Trp<\/p>\n<\/td>\n MS6318-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Ura<\/p>\n<\/td>\n MS6319-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n Double Dropout Supplements (DDO)<\/p>\n \u53cc\u7701\u5374\u6210\u5206\u6dfb\u52a0\u5242<\/p>\n<\/td>\n DO Supplement -His\/-Leu<\/p>\n<\/td>\n MS6320-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -His\/-Ura<\/p>\n<\/td>\n MS6321-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Leu\/-Trp<\/p>\n<\/td>\n MS6322-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Met\/-Trp<\/p>\n<\/td>\n MS6323-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Trp\/-Ura<\/p>\n<\/td>\n MS6324-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Trp\/-His<\/p>\n<\/td>\n MS6325-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Ura\/-Leu<\/p>\n<\/td>\n MS6326-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n Triple Dropout Supplements (TDO)<\/p>\n \u4e09\u7701\u5374\u6210\u5206\u6dfb\u52a0\u5242<\/p>\n<\/td>\n DO Supplement -His\/-Leu\/-Trp<\/p>\n<\/td>\n MS6331-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -His\/-Leu\/-Ura<\/p>\n<\/td>\n MS6332-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -His\/-Trp\/-Ura<\/p>\n<\/td>\n MS6333-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Leu\/-Met\/-Trp<\/p>\n<\/td>\n MS6334-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Leu\/-Trp\/-Ura<\/p>\n<\/td>\n MS6335-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -Trp\/-His\/-Ade<\/p>\n<\/td>\n MS6336-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n Quadruple Dropout Supplements (QDO) \u00a0<\/p>\n \u56db\u7701\u5374\u6210\u5206\u6dfb\u52a0\u5242\u00a0<\/p>\n<\/td>\n DO Supplement -Ade\/-His\/-Leu\/-Trp \u00a0\u00a0<\/p>\n<\/td>\n MS6341-10G<\/p>\n<\/td>\n 10g \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -His\/-Leu\/-Met\/-Trp<\/p>\n<\/td>\n MS6342-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n DO Supplement -His\/-Leu\/-Trp\/-Ura<\/p>\n<\/td>\n MS6343-10G<\/p>\n<\/td>\n 10g<\/p>\n<\/td>\n 418<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n \u56db\u3001\u9175\u6bcd\u8425\u517b\u7f3a\u9677\u5b8c\u5168\u57f9\u517b\u57fa\uff08SD+DO Supplments\uff09<\/strong><\/span><\/p>\n \u7c7b\u578b<\/p>\n<\/td>\n \u540d\u79f0<\/p>\n<\/td>\n \u8d27\u53f7<\/p>\n<\/td>\n \u89c4\u683c \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/p>\n<\/td>\n \u4ef7\u683c\uff08\u5143\uff09 \u00a0 \u00a0<\/p>\n<\/td>\n<\/tr>\n SD + DO Broth\u00a0<\/p>\n \u9175\u6bcd\u7f3a\u9677\u578b\u5b8c\u5168\u57f9\u517b\u57fa\uff08\u8089\u6c64\uff09 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/p>\n<\/td>\n SD\/-His Broth<\/p>\n<\/td>\n MS6361-5L \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Leu Broth<\/p>\n<\/td>\n MS6363-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Trp Broth<\/p>\n<\/td>\n MS6365-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Ura Broth<\/p>\n<\/td>\n MS6367-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Leu Broth<\/p>\n<\/td>\n MS6381-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Ura Broth<\/p>\n<\/td>\n MS6383-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Leu\/-Trp Broth<\/p>\n<\/td>\n MS6385-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Met\/-Trp Broth<\/p>\n<\/td>\n MS6387-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Trp\/-Ura Broth<\/p>\n<\/td>\n MS6389-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Trp\/-His Broth<\/p>\n<\/td>\n MS6391-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Trp\/-Ade Broth<\/p>\n<\/td>\n MS6393-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Ura\/-Leu Broth<\/p>\n<\/td>\n MS6395-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Leu\/-Trp Broth<\/p>\n<\/td>\n MS6411-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Leu\/-Ura Broth<\/p>\n<\/td>\n MS6413-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Trp\/-Ura Broth<\/p>\n<\/td>\n MS6415-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Leu\/-Met\/-Trp Broth<\/p>\n<\/td>\n MS6417-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Leu\/-Trp\/-Ura Broth<\/p>\n<\/td>\n MS6419-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Trp\/-His\/-Ade Broth<\/p>\n<\/td>\n MS6421-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-Ade\/-His\/-Leu\/-Trp Broth \u00a0 \u00a0 \u00a0 \u00a0<\/p>\n<\/td>\n MS6431-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Leu\/-Met\/-Trp Broth<\/p>\n<\/td>\n MS6433-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Leu\/-Trp\/-Ura Broth<\/p>\n<\/td>\n MS6435-5L<\/p>\n<\/td>\n 5L<\/p>\n<\/td>\n 838<\/p>\n<\/td>\n<\/tr>\n SD + DO with Agar\u00a0<\/p>\n \u9175\u6bcd\u7f3a\u9677\u578b\u5b8c\u5168\u57f9\u517b\u57fa\uff08\u743c\u8102\uff09<\/p>\n<\/td>\n SD\/-His with Agar<\/p>\n<\/td>\n MS6362-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Leu with Agar<\/p>\n<\/td>\n MS6364-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Trp with Agar<\/p>\n<\/td>\n MS6366-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Ura with Agar<\/p>\n<\/td>\n MS6368-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Leu with Agar<\/p>\n<\/td>\n MS6382-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Ura with Agar<\/p>\n<\/td>\n MS6384-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Leu\/-Trp with Agar<\/p>\n<\/td>\n MS6386-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Met\/-Trp with Agar<\/p>\n<\/td>\n MS6388-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Trp\/-Ura with Agar<\/p>\n<\/td>\n MS6390-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Trp\/-His with Agar<\/p>\n<\/td>\n MS6392-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Trp\/-Ade with Agar<\/p>\n<\/td>\n MS6394-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Ura\/-Leu with Agar<\/p>\n<\/td>\n MS6396-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Leu\/-Trpwith Agar<\/p>\n<\/td>\n MS6412-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Leu\/-Urawith Agar<\/p>\n<\/td>\n MS6414-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Trp\/-Urawith Agar<\/p>\n<\/td>\n MS6416-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Leu\/-Met\/-Trpwith Agar<\/p>\n<\/td>\n MS6418-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Leu\/-Trp\/-Urawith Agar<\/p>\n<\/td>\n MS6420-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Trp\/-His\/-Adewith Agar<\/p>\n<\/td>\n MS6422-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-Ade\/-His\/-Leu\/-Trpwith Agar<\/p>\n<\/td>\n MS6432-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Leu\/-Met\/-Trpwith Agar<\/p>\n<\/td>\n MS6434-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n SD\/-His\/-Leu\/-Trp\/-Urawith Agar<\/p>\n<\/td>\n MS6436-5L<\/p>\n<\/td>\n 10\u00d70.5 L<\/p>\n<\/td>\n 1078<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n \u4e94\u3001\u7b5b\u9009\u6297\u751f\u7d20\uff08\u5e95\u7269\uff09<\/strong><\/span><\/p>\n \u8d27\u53f7<\/p>\n<\/td>\n \u540d\u79f0<\/p>\n<\/td>\n \u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u4ef7\u683c\uff08\u5143\uff09\u00a0<\/p>\n<\/td>\n<\/tr>\n Aureobasidin A\uff08AbA\uff09\u91d1\u62c5\u5b50\u7d20A\uff081 mg\/ml\uff09<\/a><\/p>\n<\/td>\n 1ml<\/p>\n<\/td>\n 900<\/p>\n<\/td>\n<\/tr>\n 25mg<\/p>\n<\/td>\n 285<\/p>\n<\/td>\n<\/tr>\n 1g<\/p>\n<\/td>\n 280<\/p>\n<\/td>\n<\/tr>\n 500mg<\/p>\n<\/td>\n 316<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n \u3010\u8865\u5145\u63cf\u8ff0\u3011\uff1a<\/p>\n 1\uff09Aureobasidin A\uff08AbA\uff09\u91d1\u62c5\u5b50\u7d20A\u662f\u62a5\u544a\u57fa\u56e0AUR1-C\u7684\u7b5b\u9009\u6297\u751f\u7d20\uff0c\u4ee5\u5224\u65ad\u9175\u6bcd\u83cc\u662f\u5426\u80fd\u8868\u8fbe\u8d4b\u4e88\u5176AbA\u6297\u6027\u7684\u9176\u3002<\/p>\n 2\uff09X-\u03b1-Gal\u662f\u03b1-\u534a\u4e73\u7cd6\u82f7\u9176\uff08\u7531MEL1\u57fa\u56e0\u7f16\u7801\uff09\u7684\u5e95\u7269\uff0c\u5f53\u53cc\u6742\u4ea4\u53d1\u751f\u65f6MEL1\u62a5\u544a\u57fa\u56e0\u6b63\u5e38\u8868\u8fbe\u50ac\u5316\u5e95\u7269\u53d1\u751f\u989c\u8272\u53cd\u5e94\uff0c\u4f7f\u5f97\u9175\u6bcd\u83cc\u843d\u5448\u84dd\u8272\u3002<\/p>\n 3\uff09X-Gal\u662f\u00df-\u534a\u4e73\u7cd6\u82f7\u9176\uff08\u7531lacz\u57fa\u56e0\u7f16\u7801\uff09\u7684\u5e95\u7269\u3002<\/p>\n 4\uff095-FOA\uff085-\u6c1f\u4e73\u6e05\u9178\uff09\u9175\u6bcd\u53cd\u7b5b\u9009\uff08counter-selection\uff09\u5e95\u7269\uff0c\u5e38\u7528\u4e8e\u917f\u9152\u9175\u6bcd\uff08URA3\uff09\u7684\u9274\u5b9a\u548c\u7b5b\u9009\u3002<\/p>\n \u516d<\/strong>\u3001\u9175\u6bcd\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2<\/strong><\/span><\/p>\n \u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u4ef7\u683c\uff08\u5143\uff09\u00a0\u00a0<\/p>\n<\/td>\n<\/tr>\n 50\u6b21<\/p>\n<\/td>\n 495<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n \u3010\u8bd5\u5242\u76d2\u5de5\u4f5c\u539f\u7406\u3011\uff1a<\/strong><\/p>\n \u8be5\u8bd5\u5242\u76d2\u628a\u7845\u80f6\u67f1\u7eaf\u5316\u6280\u672f\u540c\u7ecf\u5178\u7684SDS\u78b1\u88c2\u89e3\u6cd5\u7ed3\u5408\u8d77\u6765\uff0c\u7528\u6237\u53ef\u57281\u5c0f\u65f6\u5185\u4ece\u9175\u6bcd\u57f9\u517b\u6db2\u4e2d\u7eaf\u5316\u51fa\u9ad8\u7eaf\u5ea6\u7684\u8d28\u7c92DNA\u3002\u7eaf\u5316\u8fc7\u7a0b\u4e0d\u9700\u7528\u4efb\u4f55\u5230\u6709\u5bb3\u7684\u6709\u673a\u7269\u62bd\u63d0\uff0c\u4ea6\u4e0d\u9700\u7528\u5230\u8017\u65f6\u7684\u4e59\u9187\u6c89\u6dc0\u3002\u8d28\u7c92DNA\u7684\u4ea7\u91cf\u4f9d\u8d56\u4e8e\u9175\u6bcd\u643a\u5e26\u8d28\u7c92\u7684\u62f7\u8d1d\u6570\uff0c\u9175\u6bcd\u83cc\u682a\u4ee5\u53ca\u751f\u957f\u6761\u4ef6\u3002\u4e00\u822c\u6765\u8bf4\uff0c\u9175\u6bcd\u8d28\u7c92\u7684\u62f7\u8d1d\u6570\u8f83\u4f4e\uff0c5.0ml\u8fc7\u591c\u57f9\u517b\u6db2\u4e2d\u8d28\u7c92DNA\u4ea7\u91cf\u7ea6\u4e3a1.0 \u00b5g\u5de6\u53f3\u3002\u7eaf\u5316\u7684\u8d28\u7c92\u53ef\u76f4\u63a5\u7528\u4e8e\u9176\u5207\uff0cPCR,\u7535\u8f6c\u5316\u7b49\u5e94\u7528\u3002\u9175\u6bcd\u6536\u96c6\u540e,\u52a0\u5165\u7834\u58c1\u9176\u548c\u73bb\u7483\u73e0\u53bb\u9664\u7ec6\u80de\u58c1,\u7528\u4f20\u7edf\u78b1\u88c2\u6cd5\u88c2\u89e3\u7ec6\u80de\uff0c\u7136\u540e\u5c06\u83b7\u5f97\u7684\u4e0a\u6e05\u6db2\u8f6c\u79fb\u81f3\u7ed3\u5408\u67f1\u7ed3\u5408DNA\uff0c\u7ecf\u8fc7\u4e24\u6b21\u5feb\u901f\u6d17\u6da4\uff0c\u6700\u540e\u7528\u65e0\u83cc\u6c34\u6d17\u8131\u51fa\u8d28\u7c92DNA\u3002\u00a0<\/p>\n \u4e03\u3001\u9175\u6bcd\u8f6c\u5316\u8bd5\u5242\u76d2<\/strong><\/span><\/p>\n \u8d27\u53f7<\/p>\n<\/td>\n \u540d\u79f0<\/p>\n<\/td>\n \u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u4ef7\u683c\uff08\u5143\uff09\u00a0\u00a0<\/p>\n<\/td>\n<\/tr>\n Yeast Transformation Kit \u9175\u6bcd\u8f6c\u5316\u8bd5\u5242\u76d2 <\/a><\/p>\n<\/td>\n 200T<\/p>\n<\/td>\n 768<\/p>\n<\/td>\n<\/tr>\n 120 Rxns<\/p>\n<\/td>\n 1246<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n \u3010\u8865\u5145\u63cf\u8ff0\u3011\uff1a<\/strong><\/p>\n \u9175\u6bcd\u8d28\u7c92\u8f6c\u5316\u8bd5\u5242\u76d2\uff0c\u542bPEG\/liAC\u9175\u6bcd\u8f6c\u5316\u9700\u8981\u7684\u6240\u6709\u8bd5\u5242\uff0c\u64cd\u4f5c\u7b80\u5355\uff0c\u8f6c\u5316\u6548\u7387\u9ad8\u3002\u00a0<\/p>\n \u516b<\/strong>\u3001\u9175\u6bcd\u53cc\u6742\u4ea4\u83cc\u548c\u611f\u53d7\u6001\u7ec6\u80de<\/strong><\/span><\/p>\n \u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u4ef7\u683c\uff08\u5143\uff09<\/p>\n<\/td>\n<\/tr>\n MF2351-300UL<\/p>\n<\/td>\n Y2HGold Yeast Strain \u9175\u6bcd\u53cc\u6742\u4ea4\u7528\u7518\u6cb9\u83cc<\/p>\n<\/td>\n 300\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n MF2352-100UL<\/p>\n<\/td>\n Y2HGold Chemically Competent Cell\u00a0\u9175\u6bcd\u83cc\u5316\u5b66\u611f\u53d7\u6001\u7ec6\u80de<\/p>\n<\/td>\n 100\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n MF2353-300UL<\/p>\n<\/td>\n Y187 Yeast Strain\u00a0\u9175\u6bcd\u53cc\u6742\u4ea4\u7528\u7518\u6cb9\u83cc<\/p>\n<\/td>\n 300\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n MF2354-100UL<\/p>\n<\/td>\n Y187 Chemically Competent Cell\u00a0\u9175\u6bcd\u83cc\u5316\u5b66\u611f\u53d7\u6001\u7ec6\u80de<\/p>\n<\/td>\n 100\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n \u4e5d\u3001\u9175\u6bcd\u83cc\u8f7d\u4f53<\/b><\/span><\/p>\n \u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/p>\n<\/td>\n \u4ef7\u683c\uff08\u5143\uff09\u00a0\u00a0 \u00a0<\/p>\n<\/td>\n<\/tr>\n MF3601-20UL<\/p>\n<\/td>\n pGBKT7 DNA-BD Vector \u9175\u6bcd\u53cc\u6742\u4ea4\u514b\u9686\u8f7d\u4f53<\/p>\n<\/td>\n 20\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n MF3602-20UL<\/p>\n<\/td>\n pGBKT7-53 Vector \u9175\u6bcd\u53cc\u6742\u4ea4\u5bf9\u7167\u8f7d\u4f53<\/p>\n<\/td>\n 20\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n MF3603-20UL<\/p>\n<\/td>\n pGBKT7-Lam Vector\u00a0 \u9175\u6bcd\u53cc\u6742\u4ea4\u5bf9\u7167\u8f7d\u4f53<\/p>\n<\/td>\n 20\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n MF3604-20UL<\/p>\n<\/td>\n pGADT7-AD Vector \u9175\u6bcd\u53cc\u6742\u4ea4\u514b\u9686\u8f7d\u4f53<\/p>\n<\/td>\n 20\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n MF3605-20UL<\/p>\n<\/td>\n pGADT7-T Vector\u9175 \u6bcd\u53cc\u6742\u4ea4\u5bf9\u7167\u8f7d\u4f53<\/p>\n<\/td>\n 20\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n MF3606-20UL<\/p>\n<\/td>\n pBridge Vector \u9175\u6bcd\u4e09\u6742\u4ea4\u514b\u9686\u8f7d\u4f53<\/p>\n<\/td>\n 20\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n MF3609-20UL<\/p>\n<\/td>\n pGBT9 Vector \u9175\u6bcd\u53cc\u6742\u4ea4\u514b\u9686\u8f7d\u4f53<\/p>\n<\/td>\n 20\u03bcl<\/p>\n<\/td>\n \u54a8\u8be2<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n <\/p>\n <\/p>\n \u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/strong><\/span><\/p>\n <\/p>\n <\/p>\n \u4e0a\u6d77\u61cb\u5eb7\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\u662f\u4e00\u5bb6\u6d89\u8db3\u4e8e\u751f\u547d\u79d1\u5b66\u548c\u751f\u7269\u6280\u672f\u9886\u57df\u7814\u7a76\u7684\u8bd5\u5242\u3001\u4eea\u5668\u548c\u5b9e\u9a8c\u5ba4\u6d88\u8017\u54c1\u4e0e\u5b9e\u9a8c\u670d\u52a1\u5de5\u4f5c\uff0c\u4e3b\u8981\u4ece\u4e8b\u7ec6\u80de\u751f\u7269\u5b66\u3001\u690d\u7269\u5b66\u3001\u5206\u5b50\u751f\u7269\u5b66\u3001\u514d\u75ab\u5b66\u3001\u751f\u7269\u5316\u5b66\u3001\u86cb\u767d\u7ec4\u5b66\u3002\u751f\u7269\u5236\u836f\u4e0e\u8bca\u65ad\u8bd5\u5242\u7814\u53d1\u751f\u4ea7\u7b49\u9886\u57df\u3002\u00a0\u672c\u516c\u53f8\u79c9\u627f\u201c\u4ee5\u4eba\u4e3a\u672c\uff0c\u4ee5\u8bda\u4e3a\u4fe1\u3001\u5408\u540c\u5b88\u4fe1\u201d\u7684\u7ecf\u8425\u7406\u5ff5\u3002\u575a\u6301”\u54c1\u8d28\u4fdd\u969c”\u7684\u539f\u5219\u4e3a\u5e7f\u5927\u5ba2\u6237\u63d0\u4f9b\u4f18\u8d28\u4ea7\u54c1\u3002<\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" \u7528\u61cb\u5eb7\u9175\u6bcd\u53cc\u6742\u4ea4\u7cfb\u5217\u4ea7\u54c1\uff0c\u5feb\u901f\u9493\u51fa\u6587\u5e93\u6df1\u5904\u7684\u9690\u5f62\u86cb\u767d\u3002\u6b22\u8fce\u7d22\u53d6\u5355\u9875\u8d44\u6599\u6216\u6765\u7535\u54a8\u8be2\u4ea7\u54c1\u4fe1\u606f\u3002<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,51],"tags":[],"_links":{"self":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/402"}],"collection":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/comments?post=402"}],"version-history":[{"count":0,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/402\/revisions"}],"wp:attachment":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media?parent=402"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/categories?post=402"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/tags?post=402"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}\n
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