{"id":25133,"date":"2019-06-30T16:46:56","date_gmt":"2019-06-30T08:46:56","guid":{"rendered":"\/\/www.562part.com\/?p=25133"},"modified":"2021-03-03T15:37:00","modified_gmt":"2021-03-03T07:37:00","slug":"%e7%bb%93%e8%82%a0%e7%82%8e%e9%80%a0%e6%a8%a1%e5%bf%85%e7%94%a8%e7%b2%be%e5%93%81%ef%bc%88mpbio%ef%bc%89-%e7%a1%ab%e9%85%b8%e8%91%a1%e8%81%9a%e7%b3%96%e9%92%a0%e7%9b%90%ef%bc%88dss%ef%bc%8cmw","status":"publish","type":"post","link":"\/\/www.562part.com\/tech-features\/25133\/","title":{"rendered":"\u7ed3\u80a0\u708e\u9020\u6a21\u5fc5\u7528\u7cbe\u54c1\uff08MPbio\uff09\u2014 \u786b\u9178\u8461\u805a\u7cd6\u94a0\u76d0\uff08DSS\uff0cMW\uff1a36000-50000 Da\uff09"},"content":{"rendered":"

\u7ed3\u80a0\u708e\u9020\u6a21\u5fc5\u7528\u7cbe\u54c1\uff08MPbio\uff0c\u524dICN\uff09<\/strong><\/span><\/p>\n

\u786b\u9178\u8461\u805a\u7cd6\u94a0\u76d0\uff08<\/strong>DSS\uff0cMW\uff1a36000-50000 Da\uff09<\/strong><\/span><\/p>\n

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/p>\n

\u57fa\u672c\u6982\u5ff5\uff1a<\/strong><\/span><\/p>\n

\u786b\u9178\u8461\u7cd6\u94a0\u76d0\uff08\u8461\u805a\u7cd6\u786b\u9178\u94a0\uff09\uff0c\u82f1\u6587Dextran Sulfate Sodium Salt\uff0c\u7f29\u5199DSS\uff0c\u662f\u8461\u805a\u7cd6\u7684\u805a\u9634\u79bb\u5b50\u884d\u751f\u7269\uff0c\u7531\u8461\u805a\u7cd6\u548c\u6c2f\u78fa\u9178\u7684\u916f\u5316\u53cd\u5e94\u5f62\u6210\u3002\u5176\u4e2d\u542b\u786b\u91cf\u7ea6\u4e3a17%\uff0c\u76f8\u5f53\u4e8e\u8461\u805a\u7cd6\u5206\u5b50\u7684\u6bcf\u4e2a\u8461\u8404\u7cd6\u6b8b\u7cd6\u4e2d\u5e73\u5747\u542b1.9\u4e2a\u786b\u9178\u57fa\u56e2\u3002<\/span><\/p>\n

DSS\uff08Mw\uff1a36000-50000 Da\uff09\u7ecf\u8bc1\u5b9e\uff083000\u7bc7\u4ee5\u4e0a\u6587\u732e\uff09\u53ef\u7528\u6765\u5efa\u7acb\u7ed3\u80a0\u708e\uff08\u708e\u6027\u80a0\u75c5IBD\uff09\u7684\u52a8\u7269\u6a21\u578b\u3002<\/strong><\/span><\/p>\n

 <\/p>\n

\u4ea7\u54c1\u7279\u70b9\uff1a<\/strong><\/span><\/p>\n

1\uff09\u00a0\u00a0\u552f\u4e00\u6027\uff1a\u672c\u5206\u5b50\u91cfDSS\u5168\u4e16\u754c\u53ea\u6709\u7f8e\u56fdMPbio\u751f\u4ea7\u5e76\u5411\u5168\u7403\u9500\u552e\uff0c\u5c5e\u65d7\u8230\u6027\uff08\u6807\u5fd7\u6027\uff09\u4ea7\u54c1\uff1b<\/span><\/p>\n

2\uff09\u00a0\u00a0\u9020\u6a21\u6210\u529f\u7387\u9ad8\uff1a\u5355\u4ece\u672c\u54c1\u6570\u4ee5\u5343\u8ba1\u7684\u5e94\u7528\u6587\u732e\uff0c\u4e14\u53d7\u5168\u7403\u5404\u9ad8\u7aef\u5b9e\u9a8c\u5ba4\u7684\u4f7f\u7528\u53ca\u63a8\u5d07\uff0c\u76f4\u63a5\u53cd\u6620\u8d28\u91cf\u53ef\u9760\u6027\uff1b<\/span><\/p>\n

3\uff09\u00a0\u00a0\u9020\u6a21\u7c7b\u578b\u591a\u6837\uff1a\u6216\u9020\u6025\u6027\u7ed3\u80a0\u708e\u6a21\u578b\uff0c\u6216\u9020\u6162\u6027\u7ed3\u80a0\u708e\u6a21\u578b\uff1b\u6216\u7ed3\u5408AOM\u5171\u540c\u5efa\u7acb\u514d\u75ab\u76f8\u5173\u7684\u7ed3\u76f4\u80a0\u764c\u6a21\u578b\uff1b<\/span><\/p>\n

4\uff09\u00a0\u00a0\u4f7f\u7528\u6781\u5176\u65b9\u4fbf\uff1a\u6c34\u6eb6\u6027\u805a\u9634\u79bb\u5b50\u590d\u5408\u7269\uff0c\u5f62\u6210\u65e0\u8272\u6f84\u6e05\u6eb6\u6db2\uff0c\u4f3c\u5929\u7136\u7c98\u591a\u7cd6\u3002\u53ea\u9700\u5f80\u996e\u7528\u6c34\u52a0\u51652%\uff5e5%\u7684DSS\u5582\u98df\u52a8\u7269\u5373\u53ef\uff1b<\/span><\/p>\n

5\uff09\u00a0\u00a0\u5b89\u5168\u6027\u9ad8\uff1a\u53ef\u88ab\u81ea\u7136\u751f\u6001\u7cfb\u7edf\u964d\u89e3\uff1b<\/span><\/p>\n

6\uff09\u00a0\u00a0\u7a33\u5b9a\u6027\u6781\u9ad8\uff1a\u5e38\u6e29\u8fd0\u8f93\u4fdd\u5b58\uff0c\u6570\u5e74\u7a33\u5b9a\uff1b<\/span><\/p>\n

\u00a0<\/strong><\/span><\/p>\n

DSS\uff08Mw\uff1a36000-50000 Da\uff09\uff08MPbio\uff09\u7528\u4e8e\u7ed3\u80a0\u708e\u9020\u6a21\u7684\u4f18\u52bf\uff1a<\/strong><\/span><\/p>\n

1\uff09\u00a0\u00a0\u63d0\u4f9b\u6700\u9ad8\u7684\u786b\u542b\u91cf\uff0818-20%\uff09\uff0c<0.2%\u7684\u6e38\u79bb\u786b\u9178\u94a0\u3010\u5f71\u54cd\u9020\u6a21\u6210\u529f\u7684\u51b3\u5b9a\u56e0\u7d20\u3011<\/span><\/p>\n

2\uff09\u00a0\u00a0\u6700\u9ad8\u624b\u6027\uff1a+104\u00b0\u6bd4\u65cb\u5149\u5ea6<\/span><\/p>\n

3\uff09\u00a0\u00a0\u6700\u4f4epH\uff1a6.2\uff081%\u6c34\u6eb6\u6db2\uff09<\/span><\/p>\n

4\uff09\u00a0\u00a0\u6700\u9ad8\u7eaf\u5ea6\uff0899%\uff09\u548c\u7a33\u5b9a\u6027<\/span><\/p>\n

5\uff09\u00a0\u00a0\u6700\u9ad8\u6709\u6548\u6027<\/span><\/p>\n

6\uff09\u00a0\u00a0\u884c\u5185\u6027\u4ef7\u6bd4\u6700\u9ad8\u9020\u6a21\u4ea7\u54c1<\/span><\/p>\n

 <\/p>\n

DSS\uff08Mw\uff1a36000-50000 Da\uff09\uff08MPbio\uff09\u4e0e\u5176\u4ed6\u54c1\u724c\u6bd4\u8f83\u6848\u4f8b<\/strong><\/span><\/p>\n

Bamba S, et al\uff082012\uff09\u5c063\u79cd\u4e0d\u540c\u7684DSS\u8fdb\u884c\u4e86\u6bd4\u8f83\u5206\u6790\uff0c\u7814\u7a76\u4e86\u5b83\u4eec\u7684\u5316\u5b66\u6027\u8d28\u548c\u7ec6\u80de\u6bd2\u6027\u4ee5\u53ca\u5f15\u53d1\u7684\u80a0\u708e\u7684\u4e25\u91cd\u6027\u3002\u4ed6\u4eec\u7684\u7814\u7a76\u7ed3\u8bba\u662f\uff0cMP Biomedicals\u7684DSS\u53ef\u5f15\u53d1\u4e00\u79cd\u4e25\u91cd\u7684\u80a0\u708e\uff0c\u8eab\u4f53\u91cd\u91cf\u8f6c\u79fb\uff0cDAI\u8bc4\u5206\uff0c\u7ed3\u80a0\u91cd\u91cf\/\u957f\u5ea6\u548c\u7ec4\u7ec7\u5b66\u8bc4\u5206\u8fd9\u4e9b\u6307\u6807\u663e\u793a\u4e86\u8fd9\u4e00\u7ed3\u679c\uff08\u89c1\u56fe1\uff0c\u6216\u76f4\u63a5\u67e5\u9605\u539f\u59cb\u6587\u732e\uff09\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u56fe1. \u7ed9\u5c0f\u9f20\u6b63\u5e38\u5582\u98df\u5e76\u6dfb\u52a02%\uff08wt\/wt\uff09\u7684DSS\u6c34\u6eb6\u6db2\uff0c\u6301\u7eed\u4e00\u5468\uff0c\u4e4b\u540e\u5904\u6b7b\u3002\u6bcf\u65e5\u76d1\u6d4b\u5355\u53ea\u5c0f\u9f20\u7684\u4f53\u91cd\u3002\u6570\u636e\u8868\u793a\u65b9\u6cd5\u4e3aSD\u6807\u51c6\u5dee\u3002\u6587\u732e\u6765\u6e90\uff1aBamba S, et al. The severity of dextran sodium sulfate-induced colitis can differ between dextran sodium sulfate preparations of the same molecular weight range. Dig Dis Sci. 2012 Feb; 57(2):327-34.<\/span><\/p>\n

 <\/p>\n

\"\"<\/span><\/p>\n

\u56fe2. \u5728\u5185\u8d28\u7f51\u538b\u529b\u4f20\u611f\u5668OASIS\u7f3a\u5931\u7684\u5c0f\u9f20\u4e0a\u4f7f\u7528DSS\u8bf1\u5bfc\u7684\u80a0\u708e\u53ef\u589e\u52a0\u5176\u6613\u611f\u6027\u3002C\uff098\u5468OASIS\u5c0f\u9f20\u7684\u5927\u80a0WB\u68c0\u6d4b\u7ed3\u679c\uff0c\u68c0\u6d4b\u4e86\u5927\u80a0\u7684\u5168\u957fOASIS\u548cOASIS\u7684N\u7aef\u3002D\uff098\u5468OASIS\u91ce\u751f\u578b\u9f20\u548cOasis-\/-\u9f20\u7684\u5927\u80a0HE\u67d3\u8272\uff08\u4e0a\u9762\uff09\u548cPAS\u67d3\u8272\uff08\u4e0b\u9762\uff09\u3002\u4e0e\u91ce\u751f\u578b\u5c0f\u9f20\u76f8\u6bd4\uff0c\u5728Oasis-\/-\u9f20\u7684\u5927\u80a0\u4e2d\u542b\u6709\u5927\u9897\u7c92\u7684\u6210\u719f\u7684\u9ecf\u819c\u676f\u72b6\u7ec6\u80de\u660e\u663e\u51cf\u5c11\u3002\u6587\u732e\u6765\u6e90\uff1aHino k,et al,(2014),.PLOS ONE 9(2):e88048. doi:10.1371\/journal.pone.0088048<\/span><\/p>\n

 <\/p>\n

DSS\uff08Mw\uff1a36000-50000 Da\uff09\uff08MPbio\uff09\u9020\u6a21\u6b65\u9aa4\uff08<\/strong>\u6587\u732e\u6765\u6e90\uff1aWirtz S., et al. Chemically induced mouse models of intestinal inflammation. Nat Protoc. 2007; 2(3):541-6.\uff09<\/strong><\/span><\/p>\n

1\u3001DSS\u6eb6\u6db2\u5236\u5907\uff082% DSS\u6eb6\u6db2\uff09\uff1a<\/strong>\u6eb6\u89e310gDSS\u4e8e500ml\u65e0\u83cc\u7684\u996e\u7528\u6c34\u4e2d\uff0c\u4f7f\u7528\u524d\u6eb6\u6db2\u4fdd\u5b58\u57284\u2103\uff0c\u6700\u597d\u73b0\u914d\u73b0\u7528\u3002<\/span><\/p>\n

 <\/p>\n

2\u3001\u5c0f\u9f20\u6025\u6027\u7ed3\u80a0\u708e\u9020\u6a21(DSS, Mw: 36000-50000 Da)<\/strong><\/span><\/p>\n

1\uff09\u00a0\u00a0\u7b2c1\u5929\u79f0\u91cd\u548c\u6807\u8bb0\u5c0f\u9f20\uff1b<\/span><\/p>\n

2\uff09\u00a0\u00a0\u52a0DSS\u6eb6\u6db2\u704c\u6ee1\u5c0f\u9f20\u7b3c\u5185\u7684\u996e\u6c34\u69fd\uff0c\u4ee55ml DSS\u6eb6\u6db2\/\u5c0f\u9f20\/\u5929\u52a0\u91cf\uff0c\u5bf9\u7167\u5c0f\u9f20\u52a0\u7b49\u4f53\u79ef\u4e0d\u542bDSS\u7684\u996e\u7528\u6c34\uff1b<\/span><\/p>\n

3\uff09\u00a0\u00a0\u7b2c3\u5929\u6e05\u7a7a\u7b3c\u5b50\u5185\u5269\u4f59\u7684DSS\u996e\u7528\u6c34\uff0c\u6bcf\u4e24\u5929\u91cd\u65b0\u88c5\u6ee1DSS\u6eb6\u6db2\uff1b<\/span><\/p>\n

4\uff09\u00a0\u00a0\u7b2c8\u5929\u6e05\u7a7a\u7b3c\u5b50\u5185\u5269\u4f59\u7684DSS\u6eb6\u6db2\uff0c\u6362\u4e0a\u4e0d\u542bDSS\u7684\u65e0\u83cc\u6c34\uff1b<\/span><\/p>\n

 <\/p>\n

3\u3001\u5c0f\u9f20\u6162\u6027\u7ed3\u80a0\u708e\u9020\u6a21\uff08DSS\uff0cMw\uff1a36000-50000 Da\uff09<\/strong><\/span><\/p>\n

1\uff09\u00a0\u00a0\u6309\u7167\u6025\u6027\u7ed3\u80a0\u708e\u9020\u6a21\u6b65\u9aa4\u8fdb\u884c\u5230\u7b2c3\uff09\u6b65\uff1b<\/span><\/p>\n

2\uff09\u00a0\u00a0\u7b2c8\u5929\u6e05\u7a7a\u7b3c\u5b50\u5185\u5269\u4f59\u7684DSS\u6eb6\u6db2\uff0c\u6362\u4e0a\u4e0d\u542bDSS\u7684\u65e0\u83cc\u6c34\u6301\u7eed\u523014\u5929\uff1b<\/span><\/p>\n

3\uff09\u00a0\u00a0\u7b2c22-26\u5929\u91cd\u590d\u6025\u6027\u7ed3\u80a0\u708e\u9020\u6a21\u6b65\u9aa4\u4e2d\u7684\u7b2c2\uff09-5\uff09\u6b65\uff1b<\/span><\/p>\n

4\uff09\u00a0\u00a0\u7b2c29\u5929\u6e05\u7a7a\u7b3c\u5b50\u5185\u5269\u4f59\u7684DSS\u6eb6\u6db2\uff0c\u6362\u4e0a\u4e0d\u542bDSS\u7684\u65e0\u83cc\u6c34\u6301\u7eed\u523014\u5929\uff1b<\/span><\/p>\n

5\uff09\u00a0\u00a0\u7b2c43-47\u5929\u91cd\u590d\u6025\u6027\u7ed3\u80a0\u708e\u9020\u6a21\u6b65\u9aa4\u4e2d\u7684\u7b2c2\uff09-5\uff09\u6b65\uff1b<\/span><\/p>\n

6\uff09\u00a0\u00a0\u7b2c50\u5929\u7528\u65e0\u83cc\u6c34\u6362\u6389\u7b3c\u5185\u5269\u4f59\u7684DSS\u6eb6\u6db2\uff1b<\/span><\/p>\n

 <\/p>\n

DSS\uff08Mw\uff1a36000-50000 Da\uff09\u8ba2\u8d2d\u4fe1\u606f\uff1a\u7279\u4ef7<\/strong>\u4fc3\u9500\uff0c\u91cf\u5927\u4ef7\u66f4\u4f18\uff0c\u6b22\u8fce\u54a8\u8be2\u3002QQ\uff1a2716000500\uff0c\u5fae\u4fe1jiqibio\u3002<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n
\u00a0\u54c1\u724c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\u00a0\u8d27\u53f7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\u00a0\u540d\u79f0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\u89c4\u683c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/span><\/td>\n\u76ee\u5f55\u4ef7\uff08\u5143\uff09\u00a0 <\/strong><\/span><\/td>\n\u4fc3\u9500\u6d3b\u52a8\u00a0<\/strong>\u00a0\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
\u00a0MPbio<\/span><\/td>\n\u00a00216011010<\/span><\/td>\n\u00a0DSS\uff08Mw\uff1a36000-50000 Da\uff09\u786b\u9178\u8461\u805a\u7cd6\u94a0\u76d0<\/a><\/span><\/td>\n10g<\/span><\/td>\n2178<\/span><\/td>\n\n

\u00a05\u6298\u4fc3\u9500\uff0c<\/span><\/strong><\/span><\/p>\n

\u00a0\u73b0\u8d27\u4f9b\u5e94<\/strong><\/span><\/p>\n

\uff08100g,500g\u89c4\u683c\uff09<\/span><\/p>\n<\/td>\n<\/tr>\n

\u00a0MPbio<\/span><\/td>\n\u00a00216011050<\/span><\/td>\n\u00a0DSS\uff08Mw\uff1a36000-50000 Da\uff09\u786b\u9178\u8461\u805a\u7cd6\u94a0\u76d0<\/a><\/span><\/td>\n50g<\/span><\/td>\n5445<\/span><\/td>\n<\/tr>\n
\u00a0MPbio<\/span><\/td>\n\u00a00216011080<\/span><\/td>\n\u00a0DSS\uff08Mw\uff1a36000-50000 Da\uff09\u786b\u9178\u8461\u805a\u7cd6\u94a0\u76d0<\/a><\/span><\/td>\n100g<\/span><\/td>\n8167.5<\/span><\/td>\n<\/tr>\n
\u00a0MPbio<\/span><\/td>\n\u00a00216011090<\/span><\/td>\n\u00a0DSS\uff08Mw\uff1a36000-50000 Da\uff09\u786b\u9178\u8461\u805a\u7cd6\u94a0\u76d0<\/a><\/span><\/td>\n500g<\/span><\/td>\n33759<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u6ce8\u610f\u4e8b\u9879<\/strong><\/span><\/p>\n

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\u00a0\u2014 \u2014 Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\u6240\u6709\u3011<\/span><\/p>\n

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