{"id":25062,"date":"2019-11-07T14:43:22","date_gmt":"2019-11-07T06:43:22","guid":{"rendered":"\/\/www.562part.com\/?p=25062"},"modified":"2020-05-13T19:26:27","modified_gmt":"2020-05-13T11:26:27","slug":"%e7%be%8e%e5%9b%bdvectorlabs-%e8%a1%80%e7%ae%a1%e5%8a%9f%e8%83%bd%e7%a0%94%e7%a9%b6%e6%a0%87%e5%bf%97%e7%89%a9-%e7%95%aa%e8%8c%84%e5%87%9d%e9%9b%86%e7%b4%a0%ef%bc%88tomato-lectin%ef%bc%89%e4%ba%a7","status":"publish","type":"post","link":"\/\/www.562part.com\/tech-features\/25062\/","title":{"rendered":"\u7f8e\u56fdVectorLabs \u8840\u7ba1\u529f\u80fd\u7814\u7a76\u6807\u5fd7\u7269-\u756a\u8304\u51dd\u96c6\u7d20\uff08Tomato Lectin\uff09\u4ea7\u54c1\u4e13\u9898"},"content":{"rendered":"

\uff08Fluorescence or biotin-conjugated Lycopersicon Esculentum Lectin\uff09<\/strong><\/span><\/p>\n

\"\"<\/b><\/span><\/p>\n

1. \u641c\u7d22\u5173\u952e\u8bcd<\/strong><\/span><\/p>\n

\u756a\u8304\u51dd\u96c6\u7d20\uff08Tomato lectin\uff09\uff1bLycopersicon esculentum agglutinin\uff08LEA\uff09\uff1bIntravascular perfusion\u8840\u7ba1\u5185\u704c\u6ce8\uff1bLectin perfusion \u51dd\u96c6\u7d20\u704c\u6ce8\uff1bMicrovasculature \u5fae\u8840\u7ba1\uff1bEndothelial cells \u5185\u76ae\u7ec6\u80de\uff1b<\/span><\/p>\n

2. \u51dd\u96c6\u7d20\u7684\u57fa\u672c\u4ecb\u7ecd<\/strong><\/span><\/p>\n

\u51dd\u96c6\u7d20\uff08lectin\uff09\u662f\u7eaf\u5316\u81ea\u5404\u79cd\u5929\u7136\u6765\u6e90\uff08\u5305\u62ec\u79cd\u5b50\u3001\u690d\u7269\u6839\u90e8\u548c\u6811\u76ae\u3001\u771f\u83cc\u3001\u7ec6\u83cc\u3001\u6d77\u8349\u548c\u6d77\u7ef5\u3001\u8f6f\u4f53\u52a8\u7269\u3001\u9c7c\u5375\u3001\u810a\u690e\u52a8\u7269\u548c\u4f4e\u7b49\u810a\u690e\u4f53\u6db2\uff09\u7684\u7cd6\u86cb\u767d\u6216\u7ed3\u5408\u7cd6\u7684\u86cb\u767d\uff0c\u56e0\u80fd\u51dd\u96c6\u7ea2\u8840\u7403\uff08\u542b\u8840\u578b\u7269\u8d28\uff09\uff0c\u6545\u79f0\u4e3a\u51dd\u96c6\u7d20\u3002\u51dd\u96c6\u7d20\u4e0d\u5177\u6709\u514d\u75ab\u6e90\u6027\u548c\u9176\u7684\u6027\u8d28\uff0c\u4f46\u5177\u6709\u9ad8\u5ea6\u4e13\u4e00\u7684\u7cd6\u7ed3\u5408\u7279\u5f02\u6027\u3002\u51dd\u96c6\u7d20\u80fd\u591f\u8bc6\u522b\u7cd6\u86cb\u767d\u548c\u7cd6\u8102\u4e2d\u590d\u6742\u7684\u78b3\u6c34\u5316\u5408\u7269\u7ed3\u6784\uff0c\u4e5f\u80fd\u8bc6\u522b\u7ec6\u80de\u819c\u8868\u9762\u7684\u7cd6\u57fa\uff0c\u56e0\u6b64\uff0c\u53ef\u4f5c\u4e3a\u7814\u7a76\u7ec6\u80de\u819c\u7ed3\u6784\u7684\u6709\u5229\u5de5\u5177\u3002\u53e6\u5916\uff0c\u51dd\u96c6\u7d20\u5177\u6709\u591a\u4ef7\u7ed3\u5408\u80fd\u529b\uff0c\u80fd\u4e0e\u8367\u5149\u7d20\u3001\u9176\u3001\u751f\u7269\u7d20\u3001\u80f6\u4f53\u91d1\u7b49\u7ed3\u5408\u800c\u4e0d\u5f71\u54cd\u672c\u8eab\u751f\u7269\u6d3b\u6027\uff0c\u56e0\u6b64\uff0c\u4e5f\u5e38\u7528\u4e8e\u514d\u75ab\u7ec6\u80de\u5316\u5b66\u7684\u7814\u7a76\uff0c\u4ee5\u53ca\u80bf\u7624\u7684\u8bca\u65ad\u8bc4\u4ef7\u7b49\u3002<\/span><\/p>\n

\u51dd\u96c6\u7d20\u7684\u4f53\u5916\u5e94\u7528\u4e3b\u8981\u6709\uff1a<\/strong><\/span><\/p>\n

1\uff09\u8840\u578b\u5206\u578b\u548c\u7ea2\u7ec6\u80de\u591a\u51dd\u96c6\u7814\u7a76\uff08blood grouping and erythrocyte polyagglutination studies\uff09\uff1b<\/span><\/p>\n

2\uff09\u6dcb\u5df4\u7ec6\u80de\u7684\u4fc3\u6709\u4e1d\u5206\u88c2\uff08mitogenic stimulation of lymphocytes\uff09\uff1b<\/span><\/p>\n

3\uff09\u6dcb\u5df4\u7ec6\u80de\u4e9a\u7fa4\u7814\u7a76\uff08lymphocyte subpopulation studies\uff09\uff1b<\/span><\/p>\n

4\uff09\u7ec6\u80de\u548c\u5176\u4ed6\u9897\u7c92\u7684\u5206\u9009\uff08fractionation of cells and other particles\uff09\uff1b<\/span><\/p>\n

5\uff09\u6b63\u5e38\u548c\u75c5\u7406\u6761\u4ef6\u4e0b\u7684\u7ec4\u7ec7\u5316\u5b66\u7814\u7a76\uff08histochemical studies of normal and pathological conditions\uff09\uff1b<\/span><\/p>\n

3. \u51dd\u96c6\u7d20\u7684\u79cd\u7c7b\u548c\u7279\u5f81<\/strong><\/span><\/p>\n

\u51dd\u96c6\u7d20\uff08lectin\uff09\u79cd\u7c7b\u7e41\u591a\uff0c\u6839\u636e\u4e0d\u540c\u7279\u5f81\u5448\u73b0\u4e0d\u540c\u7684\u51dd\u96c6\u7d20\u5206\u7c7b\u7cfb\u7edf\uff0c\u5e38\u89c1\u7684\u6709\uff1a<\/span><\/p>\n

\u6839\u636e\u51dd\u96c6\u7d20\u7684\u4e9a\u57fa\u7279\u5f81\uff0c\u5927\u81f4\u5206\u4e3a\u56db\u7c7b\uff1a\u90e8\u5206\u51dd\u96c6\u7d20\uff1b\u5168\u51dd\u96c6\u7d20\uff1b\u5d4c\u5408\u51dd\u96c6\u7d20\uff1b\u8d85\u51dd\u96c6\u7d20\uff1b<\/span><\/p>\n

\u6839\u636e\u51dd\u96c6\u7d20\u7684\u6765\u6e90\uff0c\u5927\u81f4\u5206\u4e3a\u4e09\u7c7b\uff1a\u52a8\u7269\u51dd\u96c6\u7d20\uff1b\u690d\u7269\u51dd\u96c6\u7d20\uff1b\u5fae\u751f\u7269\u51dd\u96c6\u7d20\uff1b\u76ee\u524d\u5927\u591a\u6570\u4e3a\u690d\u7269\u51dd\u96c6\u7d20\u3002<\/span><\/p>\n

\u6839\u636e\u51dd\u96c6\u7d20\u7684\u7cd6\u7ed3\u5408\u7279\u5f02\u6027\uff08\u4e0d\u540c\u4e9a\u57fa\u4e13\u4e00\u7ed3\u5408\u4e0d\u540c\u7cd6\u7c7b\uff09\uff0c\u5927\u81f4\u5206\u4e3a\u516d\u7c7b\uff1a\u4e0eD-\u7518\u9732\u7cd6\u6216D-\u8461\u8404\u7cd6\u7ed3\u5408\u7684\u51dd\u96c6\u7d20\uff1b\u4e0e2-\u4e59\u9170\u6c28\u57fa-2-\u8131\u6c27-D-\u8461\u8404\u7cd6\u7ed3\u5408\u7684\u51dd\u96c6\u7d20\uff1b\u4e0e2-\u4e59\u9170\u6c28\u57fa-2-\u8131\u6c27-D-\u534a\u4e73\u7cd6\u7ed3\u5408\u7684\u51dd\u96c6\u7d20\uff1b\u4e0eD-\u4e73\u7cd6\u7ed3\u5408\u7684\u51dd\u96c6\u7d20\uff1b\u4e0eL-\u5ca9\u85fb\u7cd6\u7ed3\u5408\u7684\u51dd\u96c6\u7d20\uff1b\u4e0e\u553e\u6db2\u9178\u7ed3\u5408\u7684\u51dd\u96c6\u7d20\uff1b\u00a0<\/span><\/p>\n

\u89c1\u4e0b\u88681\uff0c\u5e38\u7528\u51e0\u79cd\u690d\u7269\u51dd\u96c6\u7d20\u7684\u751f\u7406\u7279\u5f81\uff08\u8840\u578b\u7279\u5f02\u6027\u3001\u7cd6\u7c7b\u4e13\u4e00\u6027\u3001\u7279\u5b9a\u5e94\u7528\uff09\u3002<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u51dd\u96c6\u7d20\u6765\u6e90<\/span><\/td>\n\u5e38\u7528\u7f29\u5199<\/span><\/td>\n\u4fc3\u6709\u4e1d\u5206\u88c2\u6d3b\u6027<\/span><\/td>\n\u8840\u578b\u7279\u5f02\u6027<\/span><\/td>\n\u503e\u5411\u7ed3\u5408\u7684<\/span><\/p>\n

\u7cd6\u7c7b\u4e13\u4e00\u6027<\/span><\/td>\n

\u6291\u5236\u5242\u6216<\/span><\/p>\n

\u6d17\u8131\u7cd6\u7c7b<\/span><\/td>\n

\u7279\u5b9a\u5e94\u7528<\/span><\/td>\n<\/tr>\n
\u82b1\u751f<\/span><\/p>\n

Peanut<\/span><\/td>\n

PNA<\/span><\/td>\n\u65e0<\/span><\/td>\nT antigen (M\uff0cN)<\/span><\/td>\nGal\u03b23GalNAc<\/span><\/td>\nGal<\/span><\/td>\n\u5927\u80a0\u764c\u6807\u7b7e\u6297\u539f\u91cd\u5b9a\u4f4d\u7684\u68c0\u6d4b<\/span><\/td>\n<\/tr>\n
\u83dc\u8c46<\/span><\/p>\n

Phaseolus vulgaris<\/span><\/td>\n

PHA-E<\/span><\/td>\n\u6709<\/span><\/td>\nA\uff08-SA\uff09<\/span><\/td>\nGal\u03b24GlcNAc\u03b22Man\u03b16<\/span><\/p>\n

(GlcNAc\u03b24) (GlcNAc\u03b24Man\u03b13) Man\u03b24<\/span><\/td>\n

\u725b\u7532\u72b6\u817a\u7403\u86cb\u767d\uff08TG\uff09\uff0c\u4e59\u9178<\/span><\/td>\n\u7ed3\u5408\u4e2d\u67a2\u795e\u7ecf\u7cfb\u7edf\u7ec6\u80de\uff08HNK-1\u6297\u539f\uff09<\/span><\/td>\n<\/tr>\n
\u83dc\u8c46<\/span><\/p>\n

Phaseolus vulgaris<\/span><\/td>\n

PHA-L<\/span><\/td>\n\u6709<\/span><\/td>\n\u2014<\/span><\/td>\nGal\u03b24GlcNAc\u03b26(GlcNAc \u03b22Man\u03b13)Man\u03b13<\/span><\/td>\n\u725b\u7532\u72b6\u817a\u7403\u86cb\u767d\uff08TG\uff09\uff0c\u4e59\u9178<\/span><\/td>\n\u987a\u884c\u6027\u795e\u7ecf\u793a\u8e2a\uff1b\u8f6c\u79fb\u6027\u80bf\u7624\u6807\u5fd7\u7269\uff1b\u6dcb\u5df4\u56e0\u5b50\u751f\u4ea7\u7528\u7684\u6dcb\u5df4\u5206\u88c2\u539f\uff1b<\/span><\/td>\n<\/tr>\n
\u56db\u68f1\u8c46 Psophocarpus tetragonolobus II<\/span><\/td>\nPTL II, WBA II<\/span><\/td>\n\u65e0<\/span><\/td>\nO\uff08-SA\uff09<\/span><\/td>\nGalNAc, Gal<\/span><\/td>\nGalNAc<\/span><\/td>\n\u8840\u578bO\u4e2a\u4f53\u7684\u8840\u7ba1\u67d3\u8272\uff08\u4eba\uff09<\/span><\/td>\n<\/tr>\n
\u5927\u8c46 Soybean<\/span><\/td>\nSBA<\/span><\/td>\n\u6709<\/span><\/td>\nA>O>B<\/span><\/td>\n\u03b1>\u03b2GalNAc<\/span><\/td>\nGalNAc<\/span><\/td>\n\u5e72\u7ec6\u80de\u5206\u9009<\/span><\/td>\n<\/tr>\n
\u8346\u8c46 Ulex europaeus I<\/span><\/td>\nUEA I<\/span><\/td>\n\u65e0<\/span><\/td>\nO>A2<\/span><\/td>\n\u03b1-Fuc<\/span><\/td>\nL-Fuc<\/span><\/td>\n\u4eba\u5185\u76ae\u7ec6\u80de\u6807\u5fd7\u7269<\/span><\/td>\n<\/tr>\n
\u9ea6\u82bd Wheat Germ<\/span><\/td>\nWGA<\/span><\/td>\n\u65e0<\/span><\/td>\nA,B,O<\/span><\/td>\nGlcNAc<\/span><\/td>\n\u51e0\u4e01\u8d28\u6c34\u89e3\u9176\u6216GalNAc<\/span><\/td>\n\u80f0\u5c9b\u7d20\u53d7\u4f53\u7eaf\u5316\uff0c\u795e\u7ecf\u793a\u8e2a\uff0c\u7ec6\u83cc\u7ec6\u80de\u58c1\u7ed3\u5408<\/span><\/td>\n<\/tr>\n
\u756a\u8304<\/span><\/p>\n

Lycopersicon esculentum<\/span><\/td>\n

LEL,TL<\/span><\/td>\n\u65e0<\/span><\/td>\n\u975e\u7279\u5f02\u6027<\/span><\/td>\n(GlcNAc)2-4<\/span><\/td>\n\u51e0\u4e01\u8d28\u6c34\u89e3\u9176<\/span><\/td>\n\u5c0f\u9f20\u704c\u6ce8\u7814\u7a76\uff08\u4e0e\u8840\u7ba1\u5185\u76ae\u7ed3\u5408\uff09<\/span><\/td>\n<\/tr>\n
\u52a0\u7eb3\u7c7d Griffonia (Bandeiraea) simplicifolia I<\/span><\/td>\nGSL I, BSL I<\/span><\/td>\n\u65e0<\/span><\/td>\nB>>A1<\/span><\/td>\n\u03b1Gal, \u03b1GalNAc<\/span><\/td>\nGal\/GalNAc<\/span><\/td>\n\u5185\u76ae\u7ec6\u80de\u6807\u5fd7\u7269\uff08\u5c0f\u9f20\uff09<\/span><\/td>\n<\/tr>\n
\u52a0\u7eb3\u7c7d Griffonia (Bandeiraea) simplicifolia I Isolectin B4<\/span><\/td>\nGSL I \u2013 B4<\/span><\/td>\n\u65e0<\/span><\/td>\nB<\/span><\/td>\n\u03b1Gal<\/span><\/td>\nGal\u6216\u68c9\u5b50\u7cd6<\/span><\/td>\n\u975e\u7075\u957f\u7c7b\u5185\u76ae\u7ec6\u80de\u6807\u5fd7\u7269\uff1b\u795e\u7ecf\u5143\u6807\u5fd7\u7269\uff1b<\/span><\/td>\n<\/tr>\n
\u52a0\u7eb3\u7c7d\u00a0<\/span> Griffonia (Bandeiraea) simplicifolia II<\/span><\/td>\nGSL I<\/span>I,<\/span><\/p>\n

BSL II<\/span><\/td>\n

\u65e0<\/span><\/td>\nA (-SA)>>B\u00a0\u00a0(-SA)<\/span><\/td>\n\u03b1 or \u03b2GlcNAc<\/span><\/td>\n\u51e0\u4e01\u8d28\u6c34\u89e3\u9176\u6216GlcNAc<\/span><\/td>\n\u9009\u62e9\u6027\u67d3\u8272\u9ad8\u5c14\u57fa\u4f53\uff1b\u5b50\u5bab\u8840\u7ba1\u6216\u67d0\u4e9b\u764c\u75c7\u6807\u5fd7\u7269\uff1b<\/span><\/td>\n<\/tr>\n
\u7cd6\u7c7b\u7f29\u5199\uff1a<\/span><\/p>\n

Fuc: L-Fucose\uff1bMan: Mannose\uff1bGal: D-Galactose; GalNAc: N-Acetylgalactosamine;<\/span><\/p>\n

Glc: D-glucose; GlcNAc: N-Acetylglucosamine;<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

4. \u756a\u8304\u51dd\u96c6\u7d20\u7684\u57fa\u672c\u4ecb\u7ecd<\/strong><\/span><\/p>\n

\u82f1\u6587\u522b\u540d\uff1a<\/strong><\/span><\/p>\n

Lycopersicon esculentum agglutinin\uff08LEA\uff09\uff0cFITC Conjugate; Fluorescein labeled Lycopersicon Esculentum (Tomato) Lectin; FITC-LEL; FITC-LEA;<\/span><\/p>\n

\u57fa\u672c\u6982\u5ff5\uff1a<\/strong><\/span><\/p>\n

\u756a\u8304\u51dd\u96c6\u7d20\uff08Tomato lectin\uff0cLycopersicon Esculentum Lectin\uff08LEL\uff09\uff09\uff0c\u662f\u4e00\u79cd\u975e\u5e38\u7a33\u5b9a\u7684\u5355\u4e9a\u57fa\u7cd6\u86cb\u767d\uff0c\u7ea6\u542b\u6709\u7b49\u91cf\u7684\u86cb\u767d\u8d28\u548c\u78b3\u6c34\u5316\u5408\u7269\u3002\u636e\u62a5\u9053\u5176\u7cd6\u7c7b\u90e8\u5206\u753185%\u963f\u62c9\u4f2f\u7cd6\u548c15%\u534a\u4e73\u7cd6\u7ec4\u6210\uff0c\u5728\u6eb6\u6db2\u4e2d\u53ef\u80fd\u5f62\u6210\u591a\u805a\u4f53\u805a\u5408\u7269\u3002\u756a\u8304\u51dd\u96c6\u7d20\u867d\u7136\u548c\u571f\u8c46\u51dd\u96c6\u7d20\u3001\u66fc\u9640\u7f57\u51dd\u96c6\u7d20\uff08DSL\uff09\u548c\u9ea6\u80da\u51dd\u96c6\u7d20\uff08WGA\uff09\u6709\u90e8\u5206\u76f8\u540c\u7684\u7279\u5f02\u6027\uff0c\u4f46\u5728\u8bb8\u591a\u591a\u9762\u5448\u73b0\u51fa\u72ec\u7279\u4e4b\u5904\u3002\u756a\u8304\u51dd\u96c6\u7d20LEL\u80fd\u591f\u5f88\u597d\u5730\u4e0e\u8840\u578b\u7cd6\u86cb\u767d\uff08glycophorin\uff09\u548cTamm-Horsfall\u7cd6\u86cb\u767d\u7ed3\u5408\uff0c\u80fd\u591f\u6709\u6548\u7ed3\u5408\u548c\u6807\u8bb0\u556e\u9f7f\u7c7b\u52a8\u7269\u7684\u8840\u7ba1\u5185\u76ae\uff08vascular endothelium\uff09\u3002<\/span><\/p>\n

5. \u756a\u8304\u51dd\u96c6\u7d20\u7684\u72ec\u7279\u5e94\u7528<\/strong><\/span><\/p>\n

\u756a\u8304\u51dd\u96c6\u7d20\uff08Tomato lectin\uff0cLycopersicon Esculentum Lectin\uff0cLEL\uff09\u7684\u6700\u72ec\u7279\u5e94\u7528\u5728\u4e8e\u6807\u8bb0\u556e\u9f7f\u7c7b\u52a8\u7269\u7684\u8840\u7ba1\u5185\u76ae\uff0c\u901a\u8fc7\u8840\u7ba1\u5185\u704c\u6ce8\u6cd5\uff08Intravascular Perfusion Methods\uff09\u6765\u7814\u7a76\u8840\u7ba1\u7ed3\u6784\u548c\u8bc4\u4ef7\u8840\u7ba1\u529f\u80fd\u3002\u8840\u7ba1\u5185\u6ce8\u5c04\u756a\u8304\u7d20\u51dd\u96c6\u7d20\uff08LEL\uff09\u80fd\u591f\u68c0\u6d4b\u5404\u79cd\u6765\u6e90\u7ec4\u7ec7\u7684\u8840\u7ba1\u7ed3\u6784\uff08vascular structures\uff09\uff0c\u5305\u62ec\u5927\u8111\u3001\u80be\u810f\u3001\u809d\u810f\u3001\u5c0f\u80a0\u3001\u813e\u810f\u3001\u76ae\u80a4\u3001\u9aa8\u9abc\u808c\u548c\u5fc3\u808c\uff0c\u4ee5\u53ca\u5b9e\u9a8c\u6027\u80bf\u7624\u3002LEL\u7684\u8840\u7ba1\u6807\u8bb0\u4e0e\u5176\u4ed6\u5f62\u6001\u6807\u8bb0\u6280\u672f\u5305\u62ec\u514d\u75ab\u7ec4\u5316\u548c\u514d\u75ab\u7ec6\u80de\u5316\u5b66\u90fd\u517c\u5bb9\uff0c\u56e0\u6b64\u662f\u4e00\u79cd\u975e\u5e38\u7075\u654f\u548c\u6709\u6548\u7684\u65b9\u6cd5\u6765\u63ed\u793a\u4e0e\u5b9e\u8d28\u53d1\u5c55\u3001\u7ed3\u5408\u548c\u529f\u80fd\u5176\u4ed6\u65b9\u9762\u6709\u5173\u8054\u7684\u8840\u7ba1\u6a21\u5f0f\u3002<\/span><\/p>\n

\u756a\u8304\u51dd\u96c6\u7d20\uff08Tomato lectin\uff0cLycopersicon Esculentum Lectin\uff0cLEL\uff09\u4e5f\u80fd\u76f4\u63a5\u6807\u8bb0\u7ec4\u7ec7\u5207\u7247\u3002<\/span><\/p>\n

6. \u756a\u8304\u51dd\u96c6\u7d20\u7684\u4ea7\u54c1\u4e0e\u5e94\u7528\u793a\u4f8b<\/strong><\/span><\/p>\n

\u7f8e\u56fdVector Laboratories\uff08Vectorlabs\uff09\u63d0\u4f9b\u591a\u79cd\u756a\u8304\u51dd\u96c6\u7d20\uff08LEL\uff09\uff0c\u5305\u62ec\u4e0d\u5e26\u6807\u8bb0\uff0c\u751f\u7269\u7d20\u6807\u8bb0\uff0c\u8367\u5149\u6807\u8bb0\u548c\u743c\u8102\u7cd6\u5076\u8054\u7684\u4ea7\u54c1\uff0c\u6ee1\u8db3\u7528\u6237\u7684\u591a\u65b9\u4f4d\u9700\u6c42\uff0c\u5176\u6240\u6709\u4ea7\u54c1\u90fd\u7ecf\u8fc7\u4e25\u683c\u7684\u8d28\u91cf\u63a7\u5236\u4ee5\u4fdd\u8bc1\u8fbe\u5230\u7406\u60f3\u7684\u7ed3\u679c\uff0c\u5e76\u4e14\u4e5f\u88ab\u56fd\u5185\u5916\u5927\u91cf\u5b9e\u9a8c\u5ba4\u6240\u8ba4\u53ef\uff0c\u6709\u5927\u91cf\u7684\u5f15\u7528\u6587\u732e\u53ef\u505a\u53c2\u9605\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u00a0\u56fe1. \u5c0f\u9f20\u80be\u810f\u7ec4\u7ec7\uff0c\u7532\u919b\u56fa\u5b9a\uff0c\u77f3\u8721\u5305\u57cb\uff1a\u7ecfDyLight 488 Labeled Lycopersicon Esculentum (Tomato) Lectin\uff08\u8d27\u53f7\uff1aDL-1174\uff09\u6807\u8bb0\u5e76\u7528Vectashield Hardset with DAPI\u5c01\u7247\u7684\u67d3\u8272\u56fe\u7247\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u00a0\u56fe2. \u5c0f\u9f20\u89c6\u7f51\u819c\u5e73\u8d34\uff0c\u704c\u6ce8Dylight 594-LEL\uff08\u8d27\u53f7\uff1aDL-1177\uff09\uff0c\u89c6\u7f51\u819c\u7528Vectashield HardSet+DAPI\u5c01\u7247\u3002\uff08George W. Smith, Florida Atlantic University.\uff09<\/span><\/p>\n

7. <\/strong>\u756a\u8304\u51dd\u96c6\u7d20\u7684\u4ea7\u54c1\u8ba2\u8d2d<\/strong><\/span><\/p>\n

A. Vectorlab\u539f\u88c5\u6b63\u54c1\uff0c\u90e8\u5206\u73b0\u8d27\u4f9b\u5e94\uff0c\u6b22\u8fce\u6765\u7535\u54a8\u8be2\uff0c\u7535\u8bdd\uff1a021-54736159\uff0cQQ:2971634497\uff0c1563822900.\u00a0<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\u4ea7\u54c1\u540d\u79f0<\/span><\/td>\n\u4ea7\u54c1\u7f16\u53f7<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n\u76ee\u5f55\u4ef7\uff08\u5143\uff09 \u00a0<\/span><\/td>\n<\/tr>\n
Fluorescein labeled Lycopersicon Esculentum (Tomato) Lectin<\/span><\/p>\n

\uff08Ex\/Em=495\/515nm\uff0c\u7eff\u8272\u8367\u5149\uff09<\/span><\/td>\n

FL-1171<\/span><\/td>\n1mg \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/td>\n2360<\/span><\/td>\n<\/tr>\n
Texas Red labeled Lycopersicon Esculentum (Tomato) Lectin<\/span><\/p>\n

\uff08Ex\/Em=595\/615nm\uff0c\u7ea2\u8272\u8367\u5149\uff09<\/span><\/td>\n

TL-1176 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/span><\/td>\n1mg<\/span><\/td>\n2420<\/span><\/td>\n<\/tr>\n
DyLight 488 labeled Lycopersicon Esculentum (Tomato) Lectin \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/span><\/p>\n

\uff08Ex\/Em=493\/518nm\uff0c\u7eff\u8272\u8367\u5149\uff09<\/span><\/td>\n

DL-1174<\/span><\/td>\n1mg<\/span><\/td>\n2320<\/span><\/td>\n<\/tr>\n
DyLight 594 labeled Lycopersicon Esculentum (Tomato) Lectin<\/span><\/p>\n

\uff08Ex\/Em=593\/618nm\uff0c\u7ea2\u8272\u8367\u5149\uff09<\/span><\/td>\n

DL-1177<\/span><\/td>\n1mg<\/span><\/td>\n2320<\/span><\/td>\n<\/tr>\n
DyLight 649 labeled Lycopersicon Esculentum (Tomato) Lectin<\/span><\/p>\n

\uff08Ex\/Em=655\/670nm\uff0c\u8fdc\u7ea2\u8367\u5149\uff09<\/span><\/td>\n

DL-1178<\/span><\/td>\n1mg<\/span><\/td>\n2320<\/span><\/td>\n<\/tr>\n
Biotin labeled Lycopersicon Esculentum (Tomato) Lectin<\/span><\/td>\nB-1175<\/span><\/td>\n1mg<\/span><\/td>\n1800<\/span><\/td>\n<\/tr>\n
Unconjugated Lycopersicon Esculentum (Tomato) Lectin<\/span><\/td>\nL-1170<\/span><\/td>\n2mg<\/span><\/td>\n2360<\/span><\/td>\n<\/tr>\n
Agarose bound Lycopersicon Esculentum (Tomato) Lectin<\/span><\/td>\nAL-1173<\/span><\/td>\n2mg<\/span><\/td>\n3800<\/span><\/td>\n<\/tr>\n
Chitin Hydrolysate (lectin inhibitor)<\/span><\/td>\nSP-0090<\/span><\/td>\n10ml<\/span><\/td>\n1960<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

B.\u53e6\u6211\u53f8\u61cb\u5eb7\u751f\u7269\u63d0\u4f9b\u81ea\u4e3b\u54c1\u724cMKbio\u7684\u756a\u8304\u7d20\u51dd\u96c6\u7d20\u4ea7\u54c1\uff0c\u8d28\u91cf\u4fdd\u8bc1\uff0c\u73b0\u8d27\u4f9b\u5e94\uff0c\u6b22\u8fce\u6765\u7535\u54a8\u8be2\uff0c\u7535\u8bdd\uff1a021-54736159\uff0cQQ:2971634497\uff0c1563822900\u3002<\/span><\/p>\n\n\n\n\n
\u00a0\u4ea7\u54c1\u540d\u79f0<\/span><\/td>\n\u00a0\u4ea7\u54c1\u7f16\u53f7<\/span><\/td>\n\u00a0\u89c4\u683c \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/span><\/td>\n\u00a0\u76ee\u5f55\u4ef7\uff08\u5143\uff09\u00a0<\/span><\/td>\n<\/tr>\n
\u00a0FITC labeled Lycopersicon Esculentum (Tomato) Lectin \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/p>\n

\u00a0\uff08Ex\/Em=495\/515nm\uff0c\u7eff\u8272\u8367\u5149\uff09<\/span><\/td>\n

\u00a0MP6311-500UG\u00a0<\/span><\/td>\n\u00a0500\u03bcg<\/span><\/td>\n\u00a01400<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u732e<\/strong><\/span><\/p>\n

\u30101\u3011 Huang J et al. Regression of established tumors and metastases by potent vascular endothelial growth factor blockade. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7785-90. PMID: 12805568\u3010Vascular lectin perfusion, 100 \u03bcg in 100 \u03bcl of saline, fluorescein- labeled Lycopersicon esculentum lectin, Vector Lab\u3011<\/span><\/p>\n

\u30102\u3011 Inai T et al. Inhibition of vascular endothelial growth factor (VEGF) signaling in cancer causes loss of endothelial fenestrations, regression of tumor vessels, and appearance of basement membrane ghosts. Am J Pathol. 2004 Jul;165(1):35-52. PMID: 15215160\u3010Vascular lectin perfusion, 100 \u03bcg in 100 \u03bcl of 0.9% NaCl, fluorescein- labeled Lycopersicon esculentum lectin, Vector Lab\u3011<\/span><\/p>\n

\u30103\u3011 Thurston, G., et al., Cationic liposomes target angiogenic endothelial cells in tumors and chronic inflammation in mice. J. Clin Invest, 1998. 101 (7): p. 1401-1413. PMID: 9525983<\/span><\/p>\n

\u30104\u3011 Hashizume, H., et al., Openings between defective endothelial cells explain tumor vessel leakiness. Amer J Pathol, 2000. 156(4): p. 1363-1380. PMID: 10751361.\u3010Vascular perfusion, biotin- or fluorescein isothiocyanate (FITC)-labeled Lycopersicon esculentum lectin, 100 \u03bcg in 100 \u03bcl 0.9% NaCl, Vector Laboratories\u3011 \u00a0\u00a0<\/span><\/p>\n

\u30105\u3011 Debbage, P.L., et al., Intravital lectin perfusion analysis of vascular permeability in human micro- and macro- blood vessels. Histochemistry Cell Biol, 2001. 116(4): p. 349-359.\u00a0\u00a0\u00a0<\/span><\/p>\n

\u30106\u3011 Lee, J.C., et al., Interleukin-12 inhibits angiogenesis and growth of transplanted but not in situ mouse mammary tumor virus-induced mammary carcinomas. Cancer Res, 2002. 62(3): p. 747-755.\u00a0\u00a0\u00a0<\/span><\/p>\n

\u30107\u3011 Akerman, M.E., et al., Nanocrystal targeting in vivo. Proc Nat Acad Sci Usa, 2002. 99(20): p. 12617-12621.\u00a0\u00a0\u00a0<\/span><\/p>\n

\u30108\u3011Gee, M.S., et al., Tumor vessel development and maturation impose limits on the effectiveness of anti-vascular therapy. Amer J Pathol, 2003. 162(1): p. 183-193.\u00a0\u00a0\u00a0<\/span><\/p>\n

\u30109\u3011 Jilani, S.M., et al., Selective binding of lectins to embryonic chicken vasculature. J Histochem Cytochem, 2003. 51(5): p. 597-604.\u00a0\u00a0\u00a0<\/span><\/p>\n

\u301010\u3011Krasnici, S., et al., Effect of the surface charge of liposomes on their uptake by angiogenic tumor vessels. Int J Cancer, 2003. 105(4): p. 561-567.<\/span><\/p>\n

\u76f8\u5173\u4ea7\u54c1\uff1a<\/strong>\u5e38\u7528\u7ec4\u7ec7\/\u7ec6\u80de\u56fa\u5b9a\u5242\uff0c\u9002\u7528\u4e8e\u514d\u75ab\u7ec4\u7ec7\u5316\u5b66\/\u7535\u955c\u68c0\u6d4b\u3002<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u8d27\u53f7<\/span><\/td>\n\u540d\u79f0<\/span><\/td>\n\u89c4\u683c \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/span><\/td>\n\u4ef7\u683c\uff08\u5143\uff09 \u00a0<\/span><\/td>\n<\/tr>\n
MM1504-100ML \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/span><\/td>\n4% Paraformaldehyde 4%\u591a\u805a\u7532\u919b<\/span><\/td>\n100ml<\/span><\/td>\n60<\/span><\/td>\n<\/tr>\n
MM1504-500ML<\/span><\/td>\n4% Paraformaldehyde 4%\u591a\u805a\u7532\u919b<\/span><\/td>\n500ml<\/span><\/td>\n160<\/span><\/td>\n<\/tr>\n
MM1506-100ML<\/span><\/td>\n4% Paraformaldehyde (EM Grade) 4%\u591a\u805a\u7532\u919b\uff08\u7535\u955c\u4e13\u7528\uff09<\/span><\/td>\n100ml<\/span><\/td>\n80<\/span><\/td>\n<\/tr>\n
MM1506-500ML<\/span><\/td>\n4% Paraformaldehyde (EM Grade) 4%\u591a\u805a\u7532\u919b\uff08\u7535\u955c\u4e13\u7528\uff09<\/span><\/td>\n500ml<\/span><\/td>\n240<\/span><\/td>\n<\/tr>\n
MM1507-500ML<\/span><\/td>\n1% Paraformaldehyde 1%\u591a\u805a\u7532\u919b<\/span><\/td>\n500ml<\/span><\/td>\n100<\/span><\/td>\n<\/tr>\n
MM1511-500ML<\/span><\/td>\n2% Glutaraldehyde in PBS Buffer 2%\u620a\u4e8c\u919b\u56fa\u5b9a\u6db2<\/span><\/td>\n500ml<\/span><\/td>\n115<\/span><\/td>\n<\/tr>\n
MM1512-500ML<\/span><\/td>\n2.5% Glutaraldehyde in PBS Buffer 2.5%\u620a\u4e8c\u919b\u56fa\u5b9a\u6db2<\/span><\/td>\n500ml<\/span><\/td>\n125<\/span><\/td>\n<\/tr>\n
4583\uff08Sakura\uff09<\/a><\/span><\/td>\nOCT\u5305\u57cb\u5242\uff08\u51b0\u51bb\u5207\u7247\u5305\u57cb\u5242\uff09 <\/a><\/span><\/td>\n118ml<\/span><\/td>\n215<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\u00a0\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u756a\u8304\u51dd\u96c6\u7d20\uff08Tomato lectin\uff0cLycopersicon Esculentum Lectin\uff08LEL\uff09\uff09\uff0c\u662f\u4e00\u79cd\u975e\u5e38\u7a33\u5b9a\u7684\u5355\u4e9a\u57fa\u7cd6\u86cb\u767d\uff0c\u7ea6\u542b\u6709\u7b49\u91cf\u7684\u86cb\u767d\u8d28\u548c\u78b3\u6c34\u5316\u5408\u7269\u3002\u636e\u62a5\u9053\u5176\u7cd6\u7c7b\u90e8\u5206\u753185%\u963f\u62c9\u4f2f\u7cd6\u548c15%\u534a\u4e73\u7cd6\u7ec4\u6210\uff0c\u5728\u6eb6\u6db2\u4e2d\u53ef\u80fd\u5f62\u6210\u591a\u805a\u4f53\u805a\u5408\u7269\u3002<\/p>\n","protected":false},"author":1,"featured_media":26920,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[52],"tags":[],"_links":{"self":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/25062"}],"collection":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/comments?post=25062"}],"version-history":[{"count":1,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/25062\/revisions"}],"predecessor-version":[{"id":26921,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/25062\/revisions\/26921"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media\/26920"}],"wp:attachment":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media?parent=25062"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/categories?post=25062"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/tags?post=25062"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}