{"id":16787,"date":"2019-08-12T13:48:00","date_gmt":"2019-08-12T05:48:00","guid":{"rendered":"\/\/www.562part.com\/?p=16787"},"modified":"2022-02-18T15:03:44","modified_gmt":"2022-02-18T07:03:44","slug":"%e7%bb%86%e8%83%9e%e8%86%9c%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88-probes-for-cell-membrane-%e4%ba%a7%e5%93%81%e4%b8%93%e9%a2%98-%ef%bc%88dii%ef%bc%8cdio%ef%bc%8cdid%ef%bc%8cdir%ef%bc%8cdia%ef%bc%8cce","status":"publish","type":"post","link":"\/\/www.562part.com\/news\/16787\/","title":{"rendered":"\u7ec6\u80de\u819c\u8367\u5149\u63a2\u9488 (Probes for cell membrane) \u4ea7\u54c1\u4e13\u9898 \uff08DiI\uff0cDiO\uff0cDiD\uff0cDiR\uff0cDiA\uff0cCelltracker CM-DiI\uff09"},"content":{"rendered":"

\u7ec6\u80de\u819c\u8367\u5149\u63a2\u9488 (Probes for cell membrane) \u4ea7\u54c1\u4e13\u9898<\/strong><\/span><\/p>\n

\uff08DiI\uff0cDiO\uff0cDiD\uff0cDiR\uff0cDiA\uff0cCelltracker CM-DiI\uff09<\/span><\/p>\n

\u4f20\u7edf\u7ec6\u80de\u819c\u63a2\u9488\uff1a<\/strong><\/span><\/p>\n

A\uff0cDiI, DiO, DiD\u548cDiR<\/strong><\/span><\/p>\n

DiI, DiO, DiD\u548cDiR\u4f5c\u4e3a\u4e00\u7c7b\u957f\u94fe\u7684\u4eb2\u8102\u6027\u4e8c\u70f7\u57fa\u78b3\u83c1\u7c7b\u67d3\u6599(Dialkylcarbocyanines)\u8367\u5149\u67d3\u6599\u5bb6\u65cf\uff0c\u7528\u4e8e\u6807\u8bb0\u7ec6\u80de\u819c\u4ee5\u53ca\u5176\u4ed6\u8102\u6eb6\u6027\u751f\u7269\u7ed3\u6784\uff0c\u5c24\u5176\u662fDiI\uff0c\u5e7f\u6cdb\u7528\u4e8e\u6d3b\u4f53\u548c\u56fa\u5b9a\u7ec4\u7ec7\u53ca\u7ec6\u80de\u7684\u795e\u7ecf\u5143\u9006\u884c\u6027\u548c\u987a\u884c\u6027\u793a\u8e2a\u3002<\/span><\/p>\n

\u4f5c\u4e3a\u4e00\u7c7b\u73af\u5883\u654f\u611f\u578b\u8367\u5149\u67d3\u6599\uff0c\u5f53\u5b83\u4eec\u4e0e\u819c\u7ed3\u5408\u6216\u8005\u4e0e\u4eb2\u8102\u6027\u751f\u7269\u5206\u5b50\uff08\u4f8b\u5982\u86cb\u767d\u8d28\uff0c\u867d\u7136\u5728\u6c34\u4e2d\u5176\u8367\u5149\u5f3a\u5ea6\u5f88\u5f31\uff09\u7ed3\u5408\u65f6\uff0c\u5176\u8367\u5149\u5f3a\u5ea6\u663e\u8457\u589e\u5f3a\u3002\u4e00\u65e6\u8fdb\u5165\u7ec6\u80de\u540e\uff0c\u5b83\u4eec\u5728\u7ec6\u80de\u5185\u8d28\u819c\u4e2d\u9010\u6b65\u6269\u6563\uff0c\u6700\u4f73\u6d53\u5ea6\u6761\u4ef6\u4e0b\u53ef\u5c06\u6574\u4e2a\u7ec6\u80de\u67d3\u8272\u3002\u8fd9\u4e9b\u67d3\u6599\u5177\u5f88\u9ad8\u7684\u6dec\u706d\u7cfb\u6570\uff0c\u504f\u5149\u4f9d\u8d56\u6027\u548c\u5f88\u77ed\u7684\u6fc0\u53d1\u5bff\u547d\u3002<\/span><\/p>\n

\u56db\u79cd\u67d3\u6599\u5448\u73b0\u4e0d\u540c\u7684\u8367\u5149\u989c\u8272\uff0cDiI\uff08\u6a59\u8272\u8367\u5149\uff09\u3001DiO\uff08\u7eff\u8272\u8367\u5149\uff09\u3001DiD\uff08\u7ea2\u8272\u8367\u5149\uff09\u3001DiR\uff08\u6df1\u7ea2\u8272\u8367\u5149\uff09\uff0c<\/strong>\u4e3a\u6d3b\u7ec6\u80de\u591a\u8272\u5f69\u8367\u5149\u6210\u50cf\u5206\u6790\u548c\u6d41\u5f0f\u7ec6\u80de\u672f\u63d0\u4f9b\u4e86\u4e00\u79cd\u4fbf\u6377\u7684\u5de5\u5177\u3002DiO\u548cDiI\u5206\u522b\u7528\u6807\u51c6FITC\u548cTRITC\u6ee4\u5149\u5668\u68c0\u6d4b\uff0c\u53ef\u7ed3\u5408\u4f7f\u7528\u3002DiD\u53ef\u88ab633 nm\u6c26-\u6c16\uff08He-Ne\uff09\u6fc0\u5149\u6fc0\u53d1\uff0c\u5177\u6709\u6bd4DiI\u66f4\u957f\u7684\u6fc0\u53d1\u548c\u53d1\u5c04\u5149\u6ce2\u957f\uff0c\u7279\u522b\u9002\u5408\u7528\u4e8e\u6807\u8bb0\u5177\u672c\u5e95\u8367\u5149\u7684\u7ec6\u80de\u548c\u7ec4\u7ec7\u3002\u800c\uff0cDiR\u5728\u4f53\u5185\u6d3b\u4f53\u6210\u50cf\u6216\u8005\u793a\u8e2a\u4e2d\u610f\u4e49\u975e\u51e1\uff0c\u56e0\u5176\u6240\u53d1\u5c04\u7684\u7ea2\u5916\u5149\u53ef\u4ee5\u9ad8\u6548\u5730\u7a7f\u8fc7\u7ec6\u80de\u548c\u7ec4\u7ec7\uff0c\u5e76\u4e14\u5728\u7ea2\u5916\u5149\u8303\u56f4\u5185\uff0c\u5176\u672c\u5e95\u8367\u5149\u6c34\u5e73\u5f88\u4f4e\u3002<\/span><\/p>\n

B\uff0c DiA<\/strong><\/span><\/p>\n

DiA\u4e3a\u4eb2\u8102\u6027\u6c28\u57fa\u82ef\u4e59\u70ef\u57fa\u67d3\u6599\uff08dialkylaminostyryl dye\uff09\uff0c\u5e38\u7528\u4e8e\u795e\u7ecf\u5143\u7684\u793a\u8e2a\uff0c\u53ef\u4e0eDiI\u8054\u5408\u4f7f\u7528\u8fdb\u884c\u795e\u7ecf\u7ec6\u80de\u7684\u53cc\u8272\u6807\u8bb0\u3002\u7814\u7a76\u53d1\u73b0\uff0c\u67d0\u4e9b\u60c5\u51b5\u4e0b\u5f53DiO\u4e0d\u80fd\u6807\u8bb0\u56fa\u5b9a\u7ec4\u7ec7\u65f6\uff0cDiA\u53ef\u5f97\u5230\u826f\u597d\u6807\u8bb0\u6548\u679c\u3002DiA\u4e0e\u78f7\u8102\u53cc\u5c42\u819c\u7ed3\u5408\u540e\uff0c\u5149\u8c31\u8fc1\u79fb\u660e\u663e\u52a0\u5f3a\u3002\u6b63\u7531\u5176\u53d1\u5c04\u5149\u8c31\u975e\u5e38\u5e7f\uff0c\u5728\u9002\u5f53\u7684\u6ee4\u5149\u7247\u4e0b\uff0c\u53ef\u4ee5\u7eff\u8272\u3001\u6a59\u8272\uff0c\u751a\u81f3\u7ea2\u8272\u8367\u5149\u88ab\u68c0\u6d4b\u5230\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u4ea7\u54c1\u8ba2\u8d2d\u4fe1\u606f\uff1a<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
\u8d27\u53f7<\/span><\/td>\n\u4ea7\u54c1\u540d\u79f0<\/span><\/td>\nEx\/Em(nm)<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n\u4ef7\u683c\uff08\u5143\uff09<\/span><\/td>\n<\/tr>\n
MX4001-10MG<\/span><\/td>\nDiO (DiOC18(3)) \u7ec6\u80de\u819c\u7eff\u8272\u8367\u5149\u63a2\u9488<\/a><\/span><\/td>\n484\/501<\/span><\/td>\n10mg<\/span><\/td>\n558.00<\/span><\/td>\n<\/tr>\n
MX4002-10MG<\/span><\/td>\nDiI (DiIC18(3)) \u7ec6\u80de\u819c\u6a59\u7ea2\u8272\u8367\u5149\u63a2\u9488<\/a><\/span><\/td>\n550\/567<\/span><\/td>\n10mg<\/span><\/td>\n348.00<\/span><\/td>\n<\/tr>\n
MX4003-25MG<\/span><\/td>\nDiIC16(3) perchlorate \u7ec6\u80de\u819c\u6a59\u7ea2\u8272\u8367\u5149\u63a2\u9488<\/span><\/td>\n550\/567<\/span><\/td>\n25mg<\/span><\/td>\n1088.00<\/span><\/td>\n<\/tr>\n
MX4004-25MG<\/span><\/td>\nDiD Perchlorate (DiIC18(5)) \u7ec6\u80de\u819c\u7ea2\u8272\u8367\u5149\u63a2\u9488<\/a><\/span><\/td>\n644\/663<\/span><\/td>\n25mg<\/span><\/td>\n1048.00<\/span><\/td>\n<\/tr>\n
MX4005-5MG<\/span><\/td>\nDiR Iodide (DiIC18(7)) \u7ec6\u80de\u819c\u6df1\u7ea2\u8272\u8367\u5149\u63a2\u9488<\/a><\/span><\/td>\n748\/780<\/span><\/td>\n5mg<\/span><\/td>\n328.00<\/span><\/td>\n<\/tr>\n
MX4006-25MG<\/span><\/td>\nDiA \u7ec6\u80de\u819c\u8367\u5149\u63a2\u9488<\/a><\/span><\/td>\n491\/613<\/span><\/td>\n25mg<\/span><\/td>\n1188.00<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6539\u826f\u7684\u7ec6\u80de\u819c\u63a2\u9488\uff1a<\/strong><\/span><\/p>\n

C\uff0cCM-DiI\uff08\u6d3b\u7ec6\u80de\u7684\u591a\u4ee3\u793a\u8e2a\u3001\u56fa\u5b9a\u7ec6\u80de\u957f\u671f\u793a\u8e2a\uff09<\/strong><\/span><\/p>\n

CellTracker CM-DiI\u4e3aDiI\u7684\u884d\u751f\u7269\uff0c\u5728DiI\u4e2d\u52a0\u5165\u4e86\u5177\u6709\u4e2d\u5ea6\u5def\u57fa\u53cd\u5e94\u7279\u6027\u7684\u6c2f\u7532\u57fa\u53d6\u4ee3\u7269\uff0c\u540e\u8005\u80fd\u4e0e\u7ec6\u80de\u5185\u542b\u5def\u57fa\u7684\u80bd\u548c\u86cb\u767d\u7ed3\u5408\uff0c\u4f7f\u5f97CM-DiI\u80fd\u591f\u62b5\u6297\u919b\u7c7b\u7684\u56fa\u5b9a\u4f5c\u7528\u3002CM-DiI\u5728\u7ec6\u80de\u56fa\u5b9a\u3001\u7834\u819c\u53ca\u77f3\u8721\u5305\u57cb\u7684\u6574\u4e2a\u8fc7\u7a0b\u4e2d\u5747\u80fd\u5f88\u597d\u7684\u4fdd\u7559\u5728\u7ec6\u80de\u5185\uff0c\u56e0\u6b64\uff0c\u4e0e\u4f20\u7edf\u7ec6\u80de\u819c\u63a2\u9488DiI\u76f8\u6bd4\u8f83\uff0c\u66f4\u9002\u4e8e\u56fa\u5b9a\u540e\u6807\u672c\u7684\u957f\u671f\u793a\u8e2a\u3002\u5176\u7ea2\u8272\/\u7eff\u8272\u7684\u5149\u8c31\u7279\u5f81\uff0c\u4f7f\u5176\u53ef\u4e0e\u7eff\u8272\u63a2\u9488\u6216\u8005\u7eff\u8272\u8367\u5149\u86cb\u767d\u5982GFP\u4e00\u8d77\u7528\u4e8e\u53cc\u6807\u5b9e\u9a8c\u3002<\/span><\/p>\n

CellTracker CM-DiI\u5177\u4ee5\u4e0b\u7279\u5f81\uff1aa\uff09\u4f7f\u7528\u65b9\u4fbf\uff0c\u53ea\u9700\u5438\u6389\u57f9\u517b\u6db2\uff0c\u52a0\u5165\u63a2\u9488\uff0c30min\u5b75\u80b2\uff0c\u663e\u5fae\u6210\u50cf\uff1bb\uff09\u8367\u5149\u4fe1\u53f7\u81f3\u5c11\u53ef\u6301\u7eed72h\u4ee5\u4e0a\uff08\u4e00\u822c3-6\u4ee3\uff09\uff0c\u67d3\u6599\u53ea\u4f20\u5230\u5b50\u4ee3\u7ec6\u80de\uff0c\u4e0d\u4f1a\u67d3\u4e0a\u90bb\u8fd1\u7ec6\u80de\u3002c\uff09\u6bd2\u6027\u5f88\u4f4e\uff0c\u4e0d\u4f1a\u5f71\u54cd\u7ec6\u80de\u589e\u6b96\u548c\u6d3b\u529b\u3002\u603b\u800c\u8a00\u4e4b\uff0cCellTracker CM-DiI\u5177\u6709\u826f\u597d\u7684\u793a\u8e2a\u7279\u5f81\uff0c\u5bf9\u7ec6\u80de\u65e0\u6bd2\uff0c\u7a33\u5b9a\u957f\u6548\uff0c\u67d3\u6599\u5f88\u597d\u4fdd\u7559\u5728\u7ec6\u80de\u5185\uff0c\u751f\u7406pH\u8367\u5149\u4fe1\u53f7\u5f3a\u3002<\/span><\/p>\n

\u7814\u7a76\u8bc1\u5b9e\uff0cCM-DiI\u6807\u8bb0\u540e\u8367\u5149\u5728\u80de\u5185\u8868\u8fbe\u7a33\u5b9a\uff0c\u9633\u6027\u6807\u8bb0\u7387\u8fbe98%\u4ee5\u4e0a\uff0c\u6807\u8bb0\u7ec6\u80de\u5f62\u6001\u826f\u597d\uff0c\u80fd\u6709\u6548\u5730\u89c2\u5bdf\u7ec6\u80de\u5728\u4f53\u5916\u7684\u8bf1\u5bfc\u5206\u5316\u60c5\u51b5\uff1b\u6216\u5c06\u6807\u8bb0\u7684\u7ec6\u80de\u6ce8\u5165\u4f53\u5185\uff0c\u53ef\u4ee5\u6709\u6548\u7684\u663e\u793a\u79fb\u690d\u7ec6\u80de\u5728\u6d3b\u4f53\u7ec4\u7ec7\u4e2d\u7684\u8fc1\u79fb\u53ca\u5206\u5316\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u7ec6\u80de\u819c\u63a2\u9488\u5149\u8c31\u548c\u68c0\u6d4b\u6ee4\u7247\uff1a<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n
\n

\u8367\u5149\u63a2\u9488<\/span><\/p>\n<\/td>\n

\n

\u6700\u5927\u6fc0\u53d1\/\u6700\u5927\u53d1\u5c04\u6ce2\u957f<\/span><\/p>\n

\uff08Ex\/Em\uff09<\/span><\/p>\n<\/td>\n

\n

\u6ee4\u5149\u7247\u7f16\u53f7<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

Omeaga\u516c\u53f8<\/span><\/p>\n<\/td>\n

\n

Chroma\u516c\u53f8<\/span><\/p>\n<\/td>\n<\/tr>\n

DiI<\/span><\/td>\n549\/565nm<\/span><\/td>\nXF108, XF32<\/span><\/td>\n41002, 31002<\/span><\/td>\n<\/tr>\n
CM-DiI<\/span><\/td>\n553\/570nm<\/span><\/td>\nXF32<\/span><\/td>\n31002<\/span><\/td>\n<\/tr>\n
DiO<\/span><\/td>\n484\/501nm<\/span><\/td>\nXF100, XF2341001, 31001<\/span><\/td>\n41001, 31001<\/span><\/td>\n<\/tr>\n
DiD<\/span><\/td>\n644\/665nm<\/span><\/td>\nXF110, XF47<\/span><\/td>\n41008, 31023<\/span><\/td>\n<\/tr>\n
DiR<\/span><\/td>\n750\/780nm<\/span><\/td>\nXF112<\/span><\/td>\n41009<\/span><\/td>\n<\/tr>\n
DiA<\/span><\/td>\n456\/590nm<\/span><\/td>\nXF21<\/span><\/td>\n31024<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4ea7\u54c1\u8ba2\u8d2d\u4fe1\u606f\uff1a<\/strong><\/span><\/p>\n\n\n\n\n\n\n
\u8d27\u53f7<\/span><\/td>\n\u4ea7\u54c1\u540d\u79f0<\/span><\/td>\nEx\/Em (nm)<\/span><\/td>\n\u89c4\u683c<\/span><\/td>\n\u4ef7\u683c\uff08\u5143\uff09<\/span><\/td>\n<\/tr>\n
MX4007-50UG<\/span><\/td>\nCelltracker CM-DiI \u6d3b\u7ec6\u80de\u793a\u8e2a\u5242CM-DiI\uff08\u7ea2\u8272\uff09<\/a><\/span><\/td>\n553\/570<\/span><\/td>\n1\u00d750\u03bcg<\/span><\/td>\n340.00<\/span><\/td>\n<\/tr>\n
MX4007-250UG<\/span><\/td>\nCelltracker CM-DiI \u6d3b\u7ec6\u80de\u793a\u8e2a\u5242CM-DiI\uff08\u7ea2\u8272\uff09<\/a><\/span><\/td>\n553\/570<\/span><\/td>\n5\u00d750\u03bcg<\/span><\/td>\n1360.00<\/span><\/td>\n<\/tr>\n
MX4007-500UG<\/span><\/td>\nCelltracker CM-DiI \u6d3b\u7ec6\u80de\u793a\u8e2a\u5242CM-DiI\uff08\u7ea2\u8272\uff09<\/a><\/span><\/td>\n553\/570<\/span><\/td>\n10\u00d750\u03bcg<\/span><\/td>\n2380.00<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5e94\u7528\u6587\u732e\uff1a<\/strong><\/span><\/p>\n

1. Hemmrich K<\/a>et al,Applicability of the dyes CFSE, CM-DiI and PKH26 for tracking of human preadipocytes to evaluate adipose tissue engineering.Cells Tissues Organs.<\/a> 2006; 184(3-4):117-27.<\/span><\/p>\n

2. Andrade W, et al, The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes.J Immunol Methods. 1996 Aug 14;194(2):181-9.<\/span><\/p>\n

3. Schatteman GC<\/a>, et al,Blood-derived angioblasts accelerate blood-flow restoration in diabetic mice.J Clin Invest.<\/a> 2000 Aug; 106(4):571-8.<\/span><\/p>\n

4. Foley-Comer AJ<\/a>, et al, Evidence for incorporation of free-floating mesothelial cells as a mechanism of serosal healing.J Cell Sci.<\/a> 2002 Apr 1;115(Pt 7):1383-9.<\/span><\/p>\n

5. Djouad F<\/a>, et al,Immunosuppressive effect of mesenchymal stem cells favors tumor growth in allogeneic animals.Blood.<\/a> 2003 Nov 15;102(10):3837-44. Epub 2003 Jul 24.<\/span><\/p>\n

6. Hosie KA<\/a>, et al,Chronic excitotoxin-induced axon degeneration in a compartmented neuronal culture model.ASN Neuro.<\/a> 2012 Feb 23;4(1). pii: e00076. doi: 10.1042\/AN20110031.<\/span><\/p>\n

\u2014 \u2014Written\/Edited by V. Shallan\u3010\u7248\u6743\u5f52\u96c6\u5947\u751f\u7269\/MKBio\u61cb\u5eb7\u6240\u6709\u3011<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7ec6\u80de\u819c\u8367\u5149\u63a2\u9488 (Probes for cell membrane) \u4ea7\u54c1\u4e13\u9898 \uff08DiI\uff0cDiO\uff0cDiD\uff0cDiR\uff0cDiA\uff0cCelltracker CM-DiI\uff09 \u4f20\u7edf\u7ec6\u80de\u819c\u63a2\u9488\uff1a A\uff0cDiI, DiO, DiD\u548cDiR DiI, DiO, DiD\u548cDiR\u4f5c<\/p>\n","protected":false},"author":12,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[112,51],"tags":[],"_links":{"self":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/16787"}],"collection":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/comments?post=16787"}],"version-history":[{"count":2,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/16787\/revisions"}],"predecessor-version":[{"id":29528,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/posts\/16787\/revisions\/29528"}],"wp:attachment":[{"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/media?parent=16787"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/categories?post=16787"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.562part.com\/wp-json\/wp\/v2\/tags?post=16787"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}