描述
FD Tissue Cryoprotection Solution™是一种特殊配制的缓冲溶液，可防止组织在冷冻过程中受到冰晶的破坏*，并保留最佳的细胞形态和抗原性。事实证明，它可以极大地促进切片和各种组织学染色。此外，用此溶液处理过的组织可以在低温恒温器或滑动式切片机上轻松切成300微米厚，而不会破碎。该溶液可用于治疗用任何固定剂固定的组织。
*为了完全保护组织免受冰晶伪影的影响并保持最佳的细胞形态，必须在切片前迅速冷冻组织，例如，将组织浸入用干冰预冷至-70°C的异戊烷中 。
FD Tissue Cryoprotection Solution™ is a specifically formulated buffer solution that prevents tissue from ice crystal damage during freezing* and preserves the best possible morphology and antigenicity of cells. It has been proven to greatly facilitate both sectioning and various histological stains. In addition, tissue treated with this solution can be easily cut as thick as 300 microns on either a cryostat or a sliding microtome without shattering. The solution may be used for treatment of tissues fixed with any fixative.
*To protect the tissue completely from ice crystal artifacts and to preserve the best cell morphology, tissue must be rapidly frozen before sectioning, e.g. by immersing tissue into isopentane precooled to -70°C with dry ice .